Pp2acα

Cadmium chloride, poly-d-lysine (PDL), 4′,6-diamidino-2-phenylindole (DAPI), and protease inhibitor cocktail were purchased from Sigma (St Louis, MO, USA). Resveratrol, SP600125, U0126, and PD169136 (Sigma) were dissolved in dimethylsulfoxide to prepare 100 mM Resveratrol, 20 mM SP600125, 5 mM U0126, and 20 mM PD169136 as stock solutions, and stored at −20°C. Dulbecco’s modified Eagle’s medium, 0.05% Trypsin-EDTA, NEUROBASAL™ Media, and B27 Supplement were purchased from Invitrogen (Grand Island, NY, USA). Horse serum and fetal bovine serum were supplied by Hyclone (Logan, UT, USA). Enhanced chemiluminescence reagent was from Millipore (Billerica, MA, USA). The following antibodies were used: PP2ACα (BD Biosciences, San Jose, CA, USA), PP2A-A subunit, PP2A-B subunit (Millipore), JNK1, phospho-JNK (Thr183/Tyr185), c-Jun, phospho-c-Jun (Ser63), phospho-Erk1/2 (Thr202/Tyr204), p38, phospho-p38 (Thr180/Tyr182), cleaved-caspase-3 (Cell Signaling Technology, Beverly, MA, USA), Erk2, demethylated-PP2A, PP5, MKP-1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-PP2A (Epitomics, Burlingame, CA, USA), FLAG, hemagglutinin (HA), β-tubulin (Sigma), goat anti-rabbit IgG-horseradish peroxidase (HRP), goat anti-mouse IgG-HRP, and rabbit anti-goat IgG-HRP (Pierce, Rockford, IL, USA). Other chemicals were purchased from local commercial sources and were of analytical grade.