Bovine serum albumin (BSA) and goat antimouse polyclonal immunoglobulin G (G-mIgG) were purchased commercially (Sigma-Aldrich, St. Louis, USA). Mouse antihuman IgG (M-hIgG) were purchased from Beijing Bersee Science and Technology Co. Ltd (Beijing, China). Recombinant spike protein receptor binding domain (RBD) fused with His-tag (RBD-His) of SARS-CoV-2, angiotensin-converting enzyme 2 (ACE2), and mouse monoclonal antibody to His-tag (anti-His mAb) were purchased from Bioeast Biotech Co. Ltd (Hangzhou, China). Polystyrene-coated quantum dot nanoparticles (QDs) with a 160 nanometer diameter were purchased from Huge Biotech Ltd (Shanghai, China). Nitrocellulose (NC) membranes, sample pads, and absorbent pads were purchased from Millipore Corporation (Bedford, MA, USA). A portable fluorescence strip reader was designed by this study. For control assays, SARS-CoV-2 Ab Rapid Test Kits (AuNPs-LFIA) were purchased from Wondfo Biotech Co., Ltd. (Guangzhou, China), SARS-CoV-2 NAb test strips (AuNPs-LFIA) were purchased from Shihuier Co., Ltd. (Yangzhou, China), and ELISA kits were purchased from WANTAI BioPharm Co., Ltd. (Beijing, China).
Absorbent pad
Manufactured by Merck Group
Sourced in United States, China
Absorbent pads are laboratory equipment designed to absorb and contain liquids. They are made of highly absorbent materials and are used to protect surfaces from spills and leaks during various laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Nitrocellulose membrane, by Merck Group (10 mentions)
Bovine serum albumin (bsa), by Merck Group (10 mentions)
Sample pad, by Merck Group (7 mentions)
Nitrocellulose nc membrane, by Merck Group (6 mentions)
Tween 20, by Merck Group (6 mentions)
Glass fiber, by Merck Group (4 mentions)
N hydroxysuccinimide, by Merck Group (3 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (3 mentions)
Conjugate pad, by Merck Group (3 mentions)
Bca kit, by Thermo Fisher Scientific (3 mentions)
Milli q system, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Cm 4000 cutter, by BioDot (2 mentions)
Isobutyl chlorocarbonate, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dicyclohexylcarbodiimide, by Merck Group (2 mentions)
Freund s complete and incomplete adjuvant, by Merck Group (2 mentions)
Hypoxanthine aminopterin thymidine, by Merck Group (2 mentions)
Hypoxanthine thymidine medium, by Merck Group (2 mentions)
Peroxidase labeled goat anti mouse igg, by Merck Group (2 mentions)
26 protocols using absorbent pad
1
Fluorescent Viral Antibody Detection
2
Fluorescent Labeling of Proteins Using QDs
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Orange–red (λem = 610 nm, particle size = 24 nm) ZnCdSe/ZnS QDs were purchased from Wuhan Jiayuan Quantum Dots Corporation, Ltd. (Wuhan, China). 1-(3-dimethylaminopropyl)-3-ethylcar-bodiimide hydrochloride (EDC-HCl) and Bovine serum albumin (BSA) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). BCA Protein Assay Kits (PC0020) were purchased from Sorlarbio Life Sciences (Beijing, China). Staphylococcal protein A (SPA) was purchased from Beijing Bersee Science and Technology Corporation, Ltd. (Beijing, China). HRP-conjugated anti-His-Tag monoclonal antibody (5C3) and HRP-conjugated goat anti-mouse IgG were purchased from Abbkine Scientific Corporation, Ltd. (Wuhan, China). eECL Western Blot Kit was purchased from CWbiotech Corporation, Ltd. (Jiangsu, China). Nitrocellulose membranes, glass fiber and absorbent pads were purchased from Millipore (Bedford, MA, USA).
3
Evaluating MAWG's Inhibition of Biofilm Formation
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To assess the effectiveness of MAWG in preventing planktonic cultures from growing early adherent monolayers on driveline materials, MAWG was applied on the surface of driveline cutouts and a microbial adherence assay was carried out (Qu et al., 2020 (link)). A drip-flow biofilm reactor was then used to evaluate the effectiveness of MAWG in preventing adherent monolayer on drivelines from further developing into mature biofilms (Goeres et al., 2009 (link); Qu et al., 2020 (link)). This drip-flow biofilm reactor mimics a “wet” driveline exit-site environment by providing continuous flow of oxygen and nutrients and grows biofilms under low shear at the air-liquid interface (Goeres et al., 2009 (link)). Driveline cut-outs with attached microorganisms, prepared in the microbial adherence assay, were placed on the absorbent pads (25 mm, Millipore, Billerica, MA) with MAWG infused in the biofilm incubation chamber. Around 10% TSB as growth media were pumped through the system at 5 ml/h/channel. Biofilms were allowed to grow for 72 h at room temperature. The samples were washed three times with PBS, and were quantitatively analyzed for CFUs. The experiment was carried out in three biological repeats in duplicate.
4
BALB/c Mice Lateral Flow Assay
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Nitrocellulose membranes, absorbent pads, glass fibers and PVC plates were purchased from Millipore Corp (Billerica, MA, USA). An XYZ-3060 dispensing platform and CM4000 Guillotine Cutter (BioDot, Irvine, CA, USA) were used to prepare test strips. A membrane strip reading instrument (TSR5000) was purchased from Jiening Biotech Co., Ltd. (Shanghai, China). A Forma Series II CO2 incubator was supplied by Thermo Electron (Waltham, MA, USA). A vacuum drying oven was purchased from Shanghai Senxin Experimental Instrument Co., Ltd. (Shanghai, China). A dehumidifier was purchased from Zhuyan Biological Co., Ltd. (Nanjing, China).
BALB/c female mice were purchased from the Center of Comparative Medicine of Yangzhou University (Yangzhou, China).
BALB/c female mice were purchased from the Center of Comparative Medicine of Yangzhou University (Yangzhou, China).
5
Rapid Aflatoxin B1 Detection
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All chemicals were of analytical reagent grade or higher from Sigma-Aldrich (St. Louis, MO, USA) and were used as received unless otherwise stated. AFB1, its antigen conjugate (AFB1-BSA), 1-ethyl-3-(3-dimethylamimopropy) carbodiimide (EDC), boric acid, anti-rabbit immunoglobulin, bovine serum albumin (BSA), ovalbumin (OVA), sucrose, Tween 20, methanol, and dichloromethane were purchased from Sigma-Aldrich. The 1% (solid content, W/V) Eu(III)-marked and COOH-modified monodisperse polystyrene beads were purchased from You Ni Biotechnology Co. Ltd. (Shanghai, China). Nitrocellulose membranes, sample pads, and absorbent pads were purchased from Millipore Corp. (Bedford, MA, USA). The CTRFIA buffer contains 1% sucrose, 0.5% BSA, and 2.5% Tween 20. The food and feed samples (ready-to-eat peanuts, corn, soy sauce, vegetable oil, and mouse feed) were purchased from a local supermarket (Wuhan, China). Deionic water was obtained from a Millipore Milli-Q system and used in all experiments.
6
Sample Pad Preparation for Assays
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The sample and absorbent pads (Millipore, MA, USA) were cut into a size of 20×150 mm. The sample pad was dipped with a buffer solution containing 2% Triton X-100 in 0.05 M Tris/HCl, pH 7.5, 0.05% polyvinylpyrrolidone for 20 min at room temperature, then dried and stored in a desicator at room temperature.
7
Sensitive Zearalenone Detection in Wheat
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ZEN (100 μg/mL, dissolved in acetonitrile) was obtained from Sigma-Aldrich (China). Bovine serum albumin (BSA), sodium dodecyl benzenesulfonate (SDBS), goat anti-mouse IgG, mycose, polyethylene glycol (PEG, MW = 20,000), polyvinylpyrrolidone (PVP), sucrose, Tween-20 were purchased from the Sino-American Biotechnology Co. (Shanghai, China). The Anti-ZEN antibody was prepared in our laboratory and purified by Protein-G Sepharose Fast Flow Columns (Amersham, NJ, USA). In addition, other reagents were not inferior to analytically pure class and were purchased from DingGuo Biotech Co. (Nanjing, China). Absorbent pads, colloidal-gold particles (30 nm in diameter), conjugation pads, nitrocellulose (NC) membrane Millipore 135 and sample pads were purchased from Shanghai Kinbio Tech Co. (Shanghai, China).
Wheat samples were collected from several regions of Jiangsu province, southeast China, that are commonly heavily infected with Fusarium (S1 Table ); The survey was conducted over 2013–2015 and a total of 202 wheat samples were collected from 13 counties during harvest. The type of these wheat samples are the main varieties of the locals, such as Yangmai, Ningmai, Huaimai and so on.
Wheat samples were collected from several regions of Jiangsu province, southeast China, that are commonly heavily infected with Fusarium (
8
Development of Fluoroquinolone Antibody Assay
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MBF, N-hydroxy succinimide (NHS), N, N-dimethylformamide (DMF), Na2B4O7, 1-(3-(dimethylamino) propyl)-3-ethylcarbodiimide hydrochloride (EDC), Freund’s adjuvant, and a mouse monoclonal antibody isotyping kit were purchased from Sigma (St Louis, MO, USA). ENR, CIP, NOR, OFL, and DIF were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). HRP-conjugated goat-anti-mouse IgG was purchased from Sino-American Biotechnology Co., Ltd. (Luoyang, China). BSA and OVA were both obtained from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Filter membranes, nitrocellulose (NC) membranes, absorbent pads, and glass fibers were purchased from Millipore (Bedford, MA, USA).
9
Lateral Flow Immunoassay for Mycotoxin Detection
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Standard solutions of AFB1, ZEA, CTN, and AFB2a-BSA (bovine serum albumin) were purchased from Sigma-Aldrich (Urbana, IL, USA). ZEA-BSA was purchased from Aokin AG (Berlin, Germany). The Anti-AFB1 monoclonal antibody and Anti-ZEA monoclonal antibody were prepared in mouse ascites fluid according to previously reported methods in our laboratory. CTN-BSA and CTN antibody were obtained from Wuxi Determine Biotech Co., Ltd. (Wuxi, China). The anti-mouse IgG was purchased from Boster Bioengineering Co., Ltd. (Wuhan, China). Mannitol, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), sucrose, polyvinylpyrrolidone (PVP), and Tween-20 were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Eu-nanosphere particles Eu-nanosphere particles were obtained from Shanghai Uni-bio Biological Co. (190 ± 10 nm particle size, solid content 0.94%). The nitrocellulose membranes and absorbent pads were procured from Millipore (Bedford, OH, USA). The XYZ 3050 Biostrip Dispenser and CM 4000 Cutter (Bio-Dot, Irvine, CA, USA) were used to prepare lateral flow immunoassay.
10
Rapid and Sensitive Biomolecular Detection
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Sodium citrate tribasic dihydrate, iron(iii ) chloride hexahydrate (FeCl3·6H2O), urea, gold(iii ) chloride trihydrate (HAuCl4·3H2O), sodium borohydride (NaBH4), polyacrylamide, (3-aminopropyl)triethoxysilane (APTES), hydroxylamine hydrochloride (NH2OH·HCl), tris(2-carboxyethyl)phosphine (TCEP), potassium phosphate monobasic (KH2PO4), potassium phosphate dibasic (K2HPO4), bovine serum albumin (BSA), and Tween 20 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-E. coli O157:H7 antibody was purchased from KPL (Gaithersburg, MD, USA). Absorbent pads, NC membranes, and Amicon ultra-0.5 centrifugal filters were purchased from Millipore (Billerica, MA, USA). Real-time polymerase chain reaction (PCR) experiments were carried out using PowerChek™ EHEC real-time PCR kit (KogeneBiotech, Seoul, Korea) and analyzed using a QuantStudio™ 3 Real-Time PCR system (Applied Biosystems, Franklin Lakes, NJ, USA).
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