Anti gsk3 beta

Protein extracts were resolved through SDS-PAGE, detected using anti-FABP7, anti-beta-catenin, anti-CK1 alpha and anti-GSK3 beta (Abcam, Cambridge, MA, USA), anti-HA, anti-E-cadherin, anti-N-cadherin, and anti-vimentin (Cell Signaling, Danvers, MA, USA) antibodies, respectively. Blotted membranes were stripped and re-blotted with an anti-p84 rabbit monoclonal antibody (Sigma, St. Louis, MO, USA) or anti-GAPDH mouse monoclonal antibody (Abcam, Cambridge, MA, USA) as a loading control.

O-GlcNAc (1:1000, #12938S), OGT (1:1000, #23177S), AKT (1:1000, #90272), phospho-AKT (Ser473) (1:1000, #12694), PFKFB2 (1:1000, #13045), phospho-PFKFB2 (Ser483) (1:1000, #13064), phospho-GSK3β (Ser9) (1:1000, #9323), AS160 (1:1000, #2670), phospho-mTOR (Ser2448) (1:1000, #5536), and LC3A/B (1:1000, #12741) antibodies were purchased from CST (Cell Signaling Technology, Danvers, MA, USA). Anti-GSK3 beta (1:8000, #ab32391) and Anti-TBC1D4 (phosphor T642) (1:5000, # ab131214) antibodies were purchased from Abcam (Amyjet Scientific Inc., Upper Heyford, UK). Bax (1:2000, #60267-1-lg), Bcl-2 (1:2000, #60178-1-lg), caspase-3 (1:500, #66470-2), and mTOR (1:1000, #20657-1-AP) antibodies were purchased from Proteintech (Rosemont, IL, USA). Secondary antibodies were labeled with horseradish peroxidase goat anti-mouse IgG (1:20,000, # SA00001-1, Proteintech, USA), goat anti-rabbit IgG (1:20,000, #SA00001-2, Proteintech, USA), and goat anti-mouse IgG, IgM, IgA (1:10,000, ThermoFisher, Munich, Germany, #A-10668).