Cary 6000i

Manufactured by Agilent Technologies

Sourced in United States

The Cary 6000i is a high-performance UV-Vis-NIR spectrophotometer designed for accurate and reliable measurements across a wide range of applications. It features a wavelength range of 175-3300 nm, allowing for the analysis of a variety of samples. The instrument provides precise and reproducible data with its advanced optical design and advanced data processing capabilities.

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Lab products found in correlation

52 protocols using cary 6000i

Quantifying Nanoparticle Uptake in Cells

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AuNS or AuNR uptake by Ma was studied using UV-Vis-NIR spectrophotometry. Ma (5.0 × 10⁶) were incubated with either AuNS (4.29 × 10⁹ per mL) or an equivalent concentration of AuNR for 24 h. Following incubation, Ma were centrifuged at 600 rpm for 7 min and then washed twice with phosphate-buffered saline (PBS) to remove excess particles, and the cells then resuspended. The absorbance of the resultant cellular suspension was measured with a Varian UV-Vis-NIR spectrophotometer (Cary 6000i, Varian, Palo Alto, CA). The percentage uptake of nanoparticles was calculated by applying the following formula:

A_{M + N} / A_{N} \times 100

where A_{M+ N} is the absorbance of endocytosed nano-shells at λ = 819 nm (or nanorods at 765 nm), and A_N is the absorbance of the reference nanoshell or nanorod solution at the same wavelength.

Christie C., Madsen S.J., Peng Q, & Hirschberg H. (2017). Photothermal Therapy Employing Gold Nanoparticle-Loaded Macrophages as Delivery Vehicles: Comparing the Efficiency of Nanoshells Versus Nanorods. Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer, 36(3), 229-235.

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Determining Active Layer Thickness

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The Film thickness of the active layer was determined based on an average of 4 scans by profilometry (KLA-Tencor Alpha-step 500 surface profilometer) over a scratch on annealed (140 °C for 4 min) spin coated samples on a glass substrate. The spin coating recipe was adjusted for each sample to obtain the desired thickness.
Reflectance of the prepared devices was measured on a Varian Cary 6000i with an integrating sphere.

Almyahi F., Andersen T.R., Cooling N.A., Holmes N.P., Griffith M.J., Feron K., Zhou X., Belcher W.J, & Dastoor P.C. (2018). Optimisation of purification techniques for the preparation of large-volume aqueous solar nanoparticle inks for organic photovoltaics. Beilstein Journal of Nanotechnology, 9, 649-659.

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Characterization of ZnO Nanostructures

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The powder XRD analysis was conducted using Mo Ka radiation (40 kV, 40 mA, k = 0.7093 Å) with a speed of 60 s on the X-ray diffractometer (XRD, Agilent technologies Gemini). The morphology and the size of ZnO nanostructures were analysed using transmission electron microscopy (TEM Carl Zeiss Libra 120) at 120 keV and scanning electron microscopy (Zeiss Auriga FIB/FESEM). The Atomic concentrations and binding energies of all the elements present in ZnO nanostructures with different morphologies were obtained from X-ray photoelectron spectroscopy (XPS) using XPS-Escalab Xi+ Thermo Scientific electron spectrometer. The binding energies were corrected for the charge shift using the C 1s peak of graphitic carbon (BE = 284.6 eV) as a reference. The optical properties were determined using ultraviolet-visible spectroscopy (UV-vis spectroscopy, Varian Cary 6000i). The electrical properties were determined using Keithley source meter controlled by a Photo Emission TEC. INC (PET) I–V test system. E-beam evaporation (Kurt Lesker PVD 75 e-beam evaporator) was used to deposit Cu (100 nm). Thin films were prepared by spin coating the sample onto ozone/UV treated (Bio Force UV/Ozone Pro Cleaner) glass coverslips and silicon substrates.

Davis K., Yarbrough R., Froeschle M., White J, & Rathnayake H. (2019). Band gap engineered zinc oxide nanostructures via a sol–gel synthesis of solvent driven shape-controlled crystal growth. RSC Advances, 9(26), 14638-14648.

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Characterization of Nanostructured Materials

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The chemical composition and functional groups were analyzed using Fourier transform infrared spectroscopy (FTIR-Varian 670-IR spectrometer). The morphology and the particle size of nanostructures were analysed using transmission electron microscopy (TEM Carl Zeiss Libra 120 and JEOL2100PLUS HR-TEM with STEM/EDS) at 120 keV and 200 keV, respectively, and scanning electron microscopy (Zeiss Auriga FIB/FESEM). The thermal stability of microstructures was analyzed using the thermogravimetric analyzer Q500. Samples were heated up to 700 °C at the increment of 10°C min⁻¹ in nitrogen gas flow. The elemental composition was obtained from an elemental composition analyzer and X-ray photon spectroscopy (XPS-Escalab Xi⁺-Thermo Scientific) respectively. Brunner–Emmett–Teller (BET) method was used to determine the pore size and the pore volume density distribution of the products. The UV-visible spectra were obtained using the UV-visible spectrometer (Varian Cary 6000i). E-beam evaporation (Kurt Lesker PVD 75 e-beam evaporator) was used to deposit the Cu layer with a thickness of 100 nm. Thin films were prepared by spin coating the colloidal solution of nanomaterials and the silane precursor in ethanol onto ozone/UV treated substrates of either quartz plates or ITO coated glass substrates. Ozone/UV treatment was performed using the Bio Force UV/Ozone Pro Cleaner.

Saha S., Dawood S., Butreddy P., Pathiraja G, & Rathnayake H. (2021). Novel biodegradable low-κ dielectric nanomaterials from natural polyphenols. RSC Advances, 11(27), 16698-16705.

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DPPH Radical Scavenging Capacity of PCL and PCL/PFA Films

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The free radical scavenging activity of films of PCL and PCL/PFA blends was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH•) free radical scavenging assay. 0.004 g of DPPH was dissolved in 100 mL of ethanol to generate a 0.1 mM solution. Working solutions were prepared fresh before each experiment. 3mL of diluted DPPH• were pipetted into a polystyrene cuvette into which a small circular piece of sample (diameter 8 mm, mass ~13 mg) was previously introduced. The cuvettes were closed with a cap as soon as the diluted DPPH• was pipetted. The absorbance of the solutions containing the films was hourly measured in an automated way for 24 h using a UV–visible spectrophotometer (Cary 6000i from Varian). The capability of scavenging the radical DPPH• was calculated by using the following formula:
where A_control is the absorbance maximum centered at a wavelength of 517–526 nm of the control (ethanol solution of DPPH•), and A_sample is the absorbance maximum centered at a wavelength of 517–526 nm for the ethanolic solution of DPPH• containing the film.

Nanni G., Heredia-Guerrero J.A., Paul U.C., Dante S., Caputo G., Canale C., Athanassiou A., Fragouli D, & Bayer I.S. (2019). Poly(furfuryl alcohol)-Polycaprolactone Blends. Polymers, 11(6), 1069.

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Comprehensive Characterization of Carbon Nanodots

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Atomic force microscope (AFM, Agilent Technologies 5600 LS Series) and transmission electron microscopy (TEM, Carl Zeiss Libra 120 Plus) were used to study the size of the CNDs. Fourier transform infrared spectroscopy (FTIR, Varian 670), Raman spectroscopy (Horiba XploRA One Raman Confocal Microscope System), X-ray photoelectron spectroscopy (XPS, Thermo Fisher ESCALAB 250 Xi), and X-ray powder diffraction (XRD, Agilent Technologies Oxford Gemini) were used to determine the elemental composition and chemical structure of the CNDs. A Zetasizer nano-ZX (Malvern Instruments ZEN3600) was used to study the stability of the CNDs as a function of pH (Fisher Scientific pH 2100). Ultraviolet-visible spectroscopy (UV-Vis spectroscopy, Varian Cary 6000i) and fluorescence spectroscopy (Varian Cary Eclipse) were used to investigate the absorbance and fluorescence properties of the CNDs, respectively.

Zeng Z., Zhang W., Arvapalli D.M., Bloom B., Sheardy A., Mabe T., Liu Y., Ji Z., Chevva H., Waldeck D.H, & Wei J. (2017). A fluorescence-electrochemical study of carbon nanodots (CNDs) in bio- and photoelectronic application and energy gap investigation. Physical chemistry chemical physics : PCCP, 19(30), 20101-20109.

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Self-floating Photocatalytic Contaminant Removal

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Thanks to their
self-floating ability, the samples were floating for 7 h under 1 Sun
irradiation on the water containing mercury salt (10 mg L^–1) or/and MB (30 mg L^–1) and MO (30 mg L^–1). The concentration of Hg⁺² in the collected distillate
was measured by ICP-OES, while the concentration of the dyes was evaluated
by a UV–vis–NIR spectrophotometer (Varian Cary 6000i)
after correlating the concentration of the dyes with the absorption
intensity of the characteristic peaks of the dyes (further details
in Section S1, of the Supporting Information).

Zafar M.S., Gatto F., Mancini G., Lauciello S., Pompa P.P., Athanassiou A, & Fragouli D. (2023). Biocomposite Cryogels for Photothermal Decontamination of Water. Langmuir, 39(22), 7793-7803.

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Transparent Material Characterization Protocol

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Transparency was determined as the normalized transmittance according to the standard ASTM D1746 by using a ultraviolet (UV) spectrophotometer Varian Cary 6000i (USA) [54 ]. For this, samples were cut into rectangular pieces and placed directly in the spectrophotometer test cell. An empty test cell was used as a reference. Five measurements were taken from different samples and the results were averaged to obtain a mean value. Normalized transmittance, in percentage, was calculated as indicated below:

N o r m a l i z e d t r a n s m i t t a n c e (%) = \frac{\log % T}{b} \times 100

where %T is the transmittance at 600 nm and b is the thickness of the sample (mm).

Guzman-Puyol S., Ceseracciu L., Tedeschi G., Marras S., Scarpellini A., Benítez J.J., Athanassiou A, & Heredia-Guerrero J.A. (2019). Transparent and Robust All-Cellulose Nanocomposite Packaging Materials Prepared in a Mixture of Trifluoroacetic Acid and Trifluoroacetic Anhydride. Nanomaterials, 9(3), 368.

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Quantifying LG Oil Content in PLA/5-LG NCs

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A UV-Vis-NIR spectrophotometer by Varian (Cary 6000i, Santa Clara, CA, USA) in double beam configuration was used to study the LG oil content in the PLA/5-LG NCs. First, a calibration curve was constructed by using bare LG essential oil in acetonitrile where the amount of the oil varied from 0.234 µL to 1.875 µL. The intensity values at 240 nm (which is the highest peak of LG oil absorption) for the bare LG essential oil used to obtain the absorption spectra were: 0.18, 0.48, 1.30, and 3.50. To determine the amount of LG oil in the PLA/5-LG NC solution, the measured intensity values at 240 nm were extrapolated to the calibration curve of bare LG.

Liakos I.L., Grumezescu A.M., Holban A.M., Florin I., D’Autilia F., Carzino R., Bianchini P, & Athanassiou A. (2016). Polylactic Acid—Lemongrass Essential Oil Nanocapsules with Antimicrobial Properties. Pharmaceuticals, 9(3), 42.

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Comprehensive Material Characterization Protocol

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TEM and EELS (Gatan) examinations were performed in a JEOL 2011 microscope. XPS was collected in a PHI-5000C ESCA system (Perkin Elmer) with Mg Kα radiation (hν = 1253.6 eV). X-ray diffraction patterns were conducted on a Brucker D8 powder X-ray diffractometer employing Cu Kα radiation. Ar adsorption–desorption isotherms were obtained by Quantachrome Autosorb-iQC at 87 K. The specific surface area was calculated based on the quenched solid density functional theory). The pore size distribution was analyzed based on Barrett–Joyner–Halenda method. Optical transmission spectra were recorded in the visible range (350–800 nm) using a Varian Cary 6000i. Sheet resistance of the electrode was measured on the Keithley 2400 source meter. The thicknesses of the micrometer scale thick films were obtained a Dektak 6 M contact profilometry (Veeco Instruments). The resistivity–temperature measurements were performed with a thermal resistance tester (TRT-1000, Wuhan, China).

Zhi J., Zhou M., Zhang Z., Reiser O, & Huang F. (2021). Interstitial boron-doped mesoporous semiconductor oxides for ultratransparent energy storage. Nature Communications, 12, 445.

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