Aspartate transaminase ast test kits

DMEM was purchased from Thermo Fisher Biochemicals Co., Ltd. (USA). Foetal bovine serum, PLA2 enzyme, and penicillin were purchased from Gibco (USA). An AMV one-step RT-PCR kit was obtained from TaKaRa (China). Kuhuang injection extract powder was provided by Changshu Leiyunshang Pharmaceutical Co., Ltd. (China). A peristaltic pump was purchased from Master-Flex (German), and a 0.22 μm filter (Millex-GV) (German) was used. Sodium Deoxycholate (SDC) was obtained from VETEC (USA), and disposable indwelling intravenous needles (BD type Y 22 G) (USA) were used. A cell counting kit-8 (CCK-8) kit was purchased from Dojindo (USA), and reactive oxygen species (ROS) detection kits were purchased from Shanghai Biyuntian (China). Malondialdehyde (MDA) assay kits, superoxide dismutase (SOD) assay kits, glutathione (GSH) assay kits, and TBA assay kits were purchased from Nanjing Jiancheng Institute of Bioengineering (China). Aspartate transaminase (AST) test kits, alanine transaminase (ALT) test kits, lactate dehydrogenase (LDH) test kits, and alkaline phosphatase (ALP) test kits were all obtained from Nanjing Jiancheng Institute of Biological Engineering (China). CPZ was purchased from MCE (MedChemExpress) (USA), and GW9662 was obtained from Selleck (USA).

Antibodies against β-actin, Bcl-2 Associated X Protein (Bax), B-cell lymphoma-2 (Bcl-2), cysteinyl aspartate specific proteinase Caspase-3 (Caspase-3), cysteinyl aspartate specific proteinase Caspase-9 (Caspase-9), Tumor necrosis factor-α (TNF-α), Nuclear factor kappa B (NF-κB), AMPK-α, PPAR-α, SREBP-1c were purchased from ABGENT (San Diego, CA, USA) and other chemicals were obtained from Sigma (Shanghai, China). BCA Protein Assay Kits was obtained from Beyotime Institute of Biotechnology (Nanjing, China). Alanine aminotransferase (ALT) and Aspartate transaminase (AST) test kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). GSH-Px, malonic dialdehyde (MDA) and Superoxide Dismutase (SOD) detection kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Annexin V-FITC/PI double Staining Kit (YTHX Biotechnology Co., Ltd., Beijing, China).