Total genomic DNA was extracted using a kit (Tiangen Biotech, China). All genomic DNA solutions were stored at -20℃. According to previous studies, 6 antibiotic resistance genes (ARGs), 13 mobile genetic elements (MGEs) and gene cassettes were selected and detected by polymerase chain reaction (PCR)19 (link)–22 (link). PCR assays were carried out in 25 μL volumes containing 2 μL template DNA, 12.5 μL 2 × Taq PCR Master Mix (Tsingke, China), 8.5 μL ddH2O (Solarbio, China) and 1 μL each primer. The PCR products were separated by gel electrophoresis in a 1.5% agarose gel stained with GoldView™ (Sangon Biotech, China), visualized under ultraviolet light and photographed using a gel documentation system (BioRad, USA). The primers and amplification conditions used have been previously described in Table S1 23 (link),24 (link).
Ddh2o
Manufactured by Solarbio
Sourced in China
DdH2O is a purified water product designed for use in scientific laboratories. It is double-distilled and deionized to meet the high purity standards required for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Goldview, by Sangon (1 mentions)
Gel documentation system, by Bio-Rad (1 mentions)
Taq pcr master mix, by Tsingke (1 mentions)
Nah2po4, by Sinopharm (1 mentions)
Twistamp basic kit, by Twist Bioscience (1 mentions)
Minibest bacteria genomic dna extraction kit, by Takara Bio (1 mentions)
Cycle pure kit, by Omega Bio-Tek (1 mentions)
Na2hpo4, by Sinopharm (1 mentions)
Phosphate buffered saline (pbs), by Solarbio (1 mentions)
Sodium chloride (nacl), by Sinopharm (1 mentions)
Trisodium citrate, by Sinopharm (1 mentions)
2 protocols using ddh2o
1
Antibiotic Resistance Gene Detection
2
Lateral Flow Nucleic Acid Assay Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A TwistAmp Basic Kit was purchased from TwistDx Ltd.(Cambridge, UK). All LFNAA materials, including backing cards, glass fiber sample pads, conjugation pads, nitrocellulose membranes, and absorbent pads, were purchased from JN-Bio Co., Ltd. (Shanghai, China). Gold, trisodium citrate, NaH2PO4, Na2HPO4, and NaCl were offered by Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). The TE buffer was from Tiangen (Beijing, China). ddH2O, TAE, and PBS were provided by Solarbio (Beijing, China). Agarose and Cycle Pure Kit were bought from OMEGA (Omega Bio-Tek). The MiniBEST Bacteria Genomic DNA Extraction Kit was bought from TaKaRa (TaKaRa Bio-Inc., Japan).
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