Cells were washed with PBS, incubated with Human TruStain Fc Receptor Blocking Solution (BioLegend, 422302), and stained with the following fluorochrome-conjugated monoclonal antibodies: CD3-FITC (clone HIT3a), CD56-PE (clone HCD56), PD-1-APC (clone EH12.2H7), CD69-BV711 (clone FN50), Ki-67-PE-Cy7, and CD45-BV510 (clone HI30; all from BioLegend) and (clone 20Raj1, eBioscience). Live/dead staining was performed with Fixable Viability Dye 780 (eBioscience).
Cd69 bv711 clone fn50
Manufactured by BioLegend
Sourced in United States
CD69-BV711 (clone FN50) is a fluorescent-labeled antibody that binds to the CD69 protein, which is a marker of early T cell activation. This product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Human trustain fc receptor blocking solution, by BioLegend (1 mentions)
Ki 67 pe cy7, by BioLegend (1 mentions)
Cd45 bv510 clone hi30, by BioLegend (1 mentions)
Fixable viability dye 780, by Thermo Fisher Scientific (1 mentions)
Efluor 506, by Thermo Fisher Scientific (1 mentions)
Tnfα bv605 mab11, by BioLegend (1 mentions)
Ifn γ bv421 clone 4s b3, by BioLegend (1 mentions)
Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions)
7 aad, by BD (1 mentions)
Lsrfortessa flow cytometer, by BD (1 mentions)
2 protocols using cd69 bv711 clone fn50
1
Multiparametric Immune Profiling
2
Comprehensive Flow Cytometric Immunophenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For flow cytometric analysis of surface markers, cells were stained with the following antibodies: CD69 (BV711, clone FN50, BioLegend, San Diego, USA), PD-1 (BV650, clone EH12.2H7, BioLegend, San Diego, USA), PD-L1 (BV785, clone 29E.2A3), CD107a (APC, clone H4A3, BioLegend, San Diego, USA). iNKT cells were detected with a PBS57-CD1d tetramer (PE, National Institutes of Health Tetramer Core Facility, Atlanta, USA).
For intracellular antigens the following antibodies were used: IFN-γ (BV421, clone 4S.B3, BioLegend, San Diego, USA), TNF-α (BV605, Mab11, BioLegend, San Diego, USA).
For measurement of cell viability, the following dyes were used: fixable viability dye eFluor506 (eBioscience, San Diego, USA), fixable viability dye eFluor780 (eBioscience, San Diego, USA) or 7-AAD (BD Biosciences, Franklin Lakes, USA).
All measurements were performed on a BD LSRFortessa flow cytometer (BD Biosciences, Franklin Lakes, USA) and analyzed with FlowJo Version 10.8 (BD Biosciences, Franklin Lakes, USA).
For intracellular antigens the following antibodies were used: IFN-γ (BV421, clone 4S.B3, BioLegend, San Diego, USA), TNF-α (BV605, Mab11, BioLegend, San Diego, USA).
For measurement of cell viability, the following dyes were used: fixable viability dye eFluor506 (eBioscience, San Diego, USA), fixable viability dye eFluor780 (eBioscience, San Diego, USA) or 7-AAD (BD Biosciences, Franklin Lakes, USA).
All measurements were performed on a BD LSRFortessa flow cytometer (BD Biosciences, Franklin Lakes, USA) and analyzed with FlowJo Version 10.8 (BD Biosciences, Franklin Lakes, USA).
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