Nightowl 2 lb 983 nc 100

Manufactured by Berthold Technologies

Sourced in Germany

The NightOwl II LB 983 NC-100 is a luminometer designed for sensitive luminescence measurements. It features a photomultiplier tube detector and a temperature-controlled sample chamber. The device is capable of measuring various luminescent signals, including bioluminescence, chemiluminescence, and fluorescence.

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NightOWL LB 983 NightOWL II

3 protocols using nightowl 2 lb 983 nc 100

Imaging and Quantification of Luciferase Activity

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In vivo imaging and quantification of luciferase activity were carried out using a cooled CCD camera (NightOwl II LB 983 NC-100; Berthold Technologies) and the provided software. Seeds from representative lines were simultaneously sown and grown on 0.6% agarose or MS agar plates in the presence or absence of 5 μM PAC or 5 μM PAC plus 5 μM GA. Plates were directly transferred to a chamber at a constant temperature of 22°C and a photoperiod of 16 h light/8 h dark for 24 or 48 h. Plates were then sprayed with 100 μM luciferin and imaged after 40 min.

Sánchez-Montesino R., Bouza-Morcillo L., Marquez J., Ghita M., Duran-Nebreda S., Gómez L., Holdsworth M.J., Bassel G, & Oñate-Sánchez L. (2019). A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis. Molecular Plant, 12(1), 71-85.

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Protein-Protein Interaction Imaging in Nicotiana

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Luciferase complementation imaging (LCI) for testing the interaction of PIF5 with COP1 and SPA1 in N. benthamiana was performed as previously described with few modifications (Chen, et al. 2008 (link)). A. tumefaciens strain GV3101 was used for the injection of different constructs into N. benthamiana leaves. 1mM Luciferin was sprayed into the N. benthamiana leaves and kept in the dark for 5 min. Low-light cooled CCD camera (NightOWL IILB 983 NC 100, BERTHOLD) was used to capture luciferase luminesence. An exposure time of 2 min with 2×2 binning was used. Relative luciferase was equivalent to luminescence intensity / 0.2mm² leaf area.

Pham V.N., Kathare P.K, & Huq E. (2018). Dynamic regulation of PIF5 by COP1-SPA complex to optimize photomorphogenesis in Arabidopsis. The Plant journal : for cell and molecular biology, 96(2), 260-273.

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Bioluminescence Imaging for Tumor Monitoring

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Bioluminescence imaging was conducted using bioluminescence technology (Night OWLII LB983 NC100, Berthold, Germany). Before imaging, all mice received 2.5mg of D-luciferin (Xenogen Corporation, Hopkinton, MA) intraperitoneally. Mice receiving the tail vein injections were imaged weekly in this same way to monitor the condition of tumor growth.

Zhao L., Hou X., Feng Y., Zhang Y., Shao S., Wu X., Zhang J.(, & Zhang Z. (2024). A chronic stress-induced microbiome perturbation, highly enriched in Ruminococcaceae_UCG-014, promotes colorectal cancer growth and metastasis. International Journal of Medical Sciences, 21(5), 882-895.

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