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Negative magnetic streptavidin microbead selection

Manufactured by Miltenyi Biotec
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Negative magnetic streptavidin microbead selection is a lab equipment product that allows for the isolation of target cells or molecules by negatively selecting for unwanted cells or molecules. The product utilizes magnetic microbeads coated with streptavidin to bind and remove unwanted components from a sample, leaving the desired target for further analysis or processing.

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Lab products found in correlation

Apo brdu tunel kit, by BD (2 mentions) Facsaria 3, by BD (2 mentions) Facsaria fusion, by BD (2 mentions)

Spelling variants of this product

Streptavidin microbeads (158 citations) Magnetic microbeads (147 citations) Negative selection (51 citations) Negative magnetic selection (33 citations) Streptavidin magnetic microbeads (10 citations) Magnetic microbead selection (5 citations) Negative selection microbeads (2 citations)

4 protocols using negative magnetic streptavidin microbead selection

1

Detecting DNA Strand Breaks in cDCs

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The APO-BrdU TUNEL kit (BD) was used to detect DNA strand breaks. cDCs were prepared as described above prior to enrichment by negative magnetic streptavidin microbead selection (Miltenyi) to exclude T cells (CD3ε), B cells (B220), and NK cells (CD49b) (biotinylated antibodies from BioLegend). Cells were then stained for cell surface markers as described above prior to TUNEL analysis according to the manufacturer’s protocol.
Iberg C.A., Bourque J., Fallahee I., Son S, & Hawiger D. (2022). TNF-α sculpts a maturation process in vivo by pruning tolerogenic dendritic cells. Cell reports, 39(2), 110657.
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2

Enrichment and Isolation of Naive CD4+ T Cells

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Spleens and peripheral lymph nodes of OT-II Foxp3RFP donor mice were pooled together and enriched by depletion. CD4+ T cells were enriched by negative magnetic streptavidin microbead selection (Miltenyi), which excluded cells expressing the surface molecules CD8α, B220, CD11b, CD11c, and CD49b (biotinylated antibodies from BioLegend). Enriched Foxp3(RFP)neg CD25neg cells were sorted on FACSAria Fusion or FACSAriaIII (BD) instruments. After sorting, cells were washed twice in PBS and counted. 3–5 million cells were injected intravenously into the tail vein of each recipient mouse.
Iberg C.A., Bourque J., Fallahee I., Son S, & Hawiger D. (2022). TNF-α sculpts a maturation process in vivo by pruning tolerogenic dendritic cells. Cell reports, 39(2), 110657.
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3

Detecting DNA Strand Breaks in cDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
The APO-BrdU TUNEL kit (BD) was used to detect DNA strand breaks. cDCs were prepared as described above prior to enrichment by negative magnetic streptavidin microbead selection (Miltenyi) to exclude T cells (CD3ε), B cells (B220), and NK cells (CD49b) (biotinylated antibodies from BioLegend). Cells were then stained for cell surface markers as described above prior to TUNEL analysis according to the manufacturer’s protocol.
Iberg C.A., Bourque J., Fallahee I., Son S, & Hawiger D. (2022). TNF-α sculpts a maturation process in vivo by pruning tolerogenic dendritic cells. Cell reports, 39(2), 110657.
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4

Enrichment and Isolation of Naive CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens and peripheral lymph nodes of OT-II Foxp3RFP donor mice were pooled together and enriched by depletion. CD4+ T cells were enriched by negative magnetic streptavidin microbead selection (Miltenyi), which excluded cells expressing the surface molecules CD8α, B220, CD11b, CD11c, and CD49b (biotinylated antibodies from BioLegend). Enriched Foxp3(RFP)neg CD25neg cells were sorted on FACSAria Fusion or FACSAriaIII (BD) instruments. After sorting, cells were washed twice in PBS and counted. 3–5 million cells were injected intravenously into the tail vein of each recipient mouse.
Iberg C.A., Bourque J., Fallahee I., Son S, & Hawiger D. (2022). TNF-α sculpts a maturation process in vivo by pruning tolerogenic dendritic cells. Cell reports, 39(2), 110657.
+ Open protocol
+ Expand

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