Ek 075 40

Manufactured by Phoenix Pharmaceuticals

Sourced in United States

The EK-075-40 is a laboratory centrifuge capable of reaching high speed rotations. It is designed to separate liquid samples into their component parts based on their density differences. The device features a robust construction and a compact footprint to optimize workspace utilization.

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Lab products found in correlation

A110 1 1 for tg, by Nanjing Jiancheng (1 mentions) A111 1 1 for tc, by Nanjing Jiancheng (1 mentions)

2 protocols using ek 075 40

Quantifying Plasma LEAP2 Levels

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Human and mouse plasma LEAP2 concentrations were assessed by use of a specific enzyme immunoassay (EIA) for human LEAP2 detection (EK-075-40 Phoenix Pharmaceuticals, USA, RRID:AB_2909436, https://scicrunch.org/resources/Any/search?q=ab_2909436&l=ab_2909436). The plasma concentrations of LEAP2 were measured and calculated according to the manufacturer’s instructions.

Holm S., Husted A.S., Skov L.J., Morville T.H., Hagemann C.A., Jorsal T., Dall M., Jakobsen A., Klein A.B., Treebak J.T., Knop F.K., Schwartz T.W., Clemmensen C, & Holst B. (2022). Beta-Hydroxybutyrate Suppresses Hepatic Production of the Ghrelin Receptor Antagonist LEAP2. Endocrinology, 163(6), bqac038.

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LEAP2 Quantification in Biological Samples

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LEAP2 levels of CSF, plasma, and hypothalamic tissue samples were determined by a LEAP2 (38–77) (human)/LEAP2 (37–76) (mouse) enzyme-linked immunosorbent assay (ELISA) kit (EK-075-40, Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA) following the manufacturer’s instructions. The minimum detectable concentration of LEAP2 in this assay was 0.22 ng/ml. Hypothalamic LEAP2 content was normalized to the amount of protein.
Plasma total cholesterol (TC) or triglyceride (TG) levels were measured using the corresponding assay kits (A111-1-1 for TC and A110-1-1 for TG, Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions.

Chu G., Peng H., Yu N., Zhang Y., Lin X, & Lu Y. (2022). Involvement of POMC neurons in LEAP2 regulation of food intake and body weight. Frontiers in Endocrinology, 13, 932761.

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