Reduced β nicotinamide adenine dinucleotide 2 phosphate

Pooled human liver microsomes (HLM; n=50, mixed gender) and pooled human liver cytosol (HLCYT, mix gender) were purchased from Tebu Bio (Boechout, Belgium) and Sigma-Aldrich (Bornem, Belgium), respectively. RDP standard (98% purity) was obtained from TRC (Toronto, Canada); reduced β-nicotinamide adenine dinucleotide 2'-phosphate (NADPH; purity>95%), uridine 5'-diphosphoglucuronic acid (UDPGA; purity>98%), adenosine 3'phosphate 5'-phosphosulfate (PAPS; purity>96%), DPHP and TPHP-d 15 (used as internal standard, IS) were purchased from Sigma-Aldrich (Bornem, Belgium). The standard TPHP was purchased from Chiron AS (Trondheim, Norway). The DPHP-d 10 standard (also used as IS) was custom synthesized by Dr. Vladimir Belov (Max Planck Institute, Göttingen, Germany).
Acetonitrile, methanol and ammonium acetate were purchased from Merck KgA Chemicals (Darmstadt, Germany). para-HO-TPHP was synthesized by the Max Planck Institute (Göttingen, Germany) with a purity of 98%. meta-HO-TPHP was synthesized by the Organic Chemistry Synthesis Group from Duke University (NC, USA) and kindly donated by prof. Heather Stapleton (Duke University, NC, USA). Ultrapure water (18.2 MΩ) was obtained using an Elga LabWater water purification instrument (Saint Maurice, France). Oasis WAX cartridges (3 cc, 60 mg, 30 µm) were obtained from Waters (Milford, Massachusetts, USA).