Cd34 pe

hUC-MSC-p3 and hUC-MSC-p15 were collected and treated with 0.25% trypsin. The cells were individually stained with fluorescein isothiocyanate (FITC) or phycoerythrin (PE)-conjugated anti-marker monoclonal antibodies in 100 μl PBS for 15 min at room temperature, or for 30 min at 4°C, as recommended by the manufacturer. The antibodies used were specific for the following human antigens: CD34-PE, CD44-FITC, CD45-PE, CD73-PE, CD90-PE and CD105-PE (10 μl for 1×10⁶ cells; AbD Serotec, Raleigh, NC, USA). Cells were analyzed on a Cytometer 1.0, Cytomics™ FC500 flow cytometry system (Beckman Coulter, Brea, CA, USA). Positive cells were counted and the signals for the corresponding immunoglobulin isotypes were compared (15 (link)).

The following antibodies (AbD Serotec, Kidlington, UK) were used to determine the immunophenotype of the canine lymphomas by flow cytometry: CD3 FITC (clone CA17.2A12), CD5 FITC (clone YKIX322.3), CD11/18 FITC (clone YKIX490), MHC II FITC (clone YKIX334.2), CD14 PE (clone, TÜK4), CD21 PE (clone CA2.1D6), CD34 PE (clone 1H6), and CD45 APC (clone YKIX716.13). The same panel was used to verify the immunophenotype of the CLBL-1 cells (Table S3).