Fitc conjugated rat anti mouse ifn γ clone xmg1.2

HPV18-E6aa67-75 peptide, KCIDFYSRI, was synthesized by GenScript (Piscataway, NJ). The peptide’s identity and purity were validated and confirmed via mass spectrometric analysis and high-performance liquid chromatography, respectively. FITC-conjugated rat anti-mouse IFN-γ (clone XMG1.2) and PE-conjugated anti-mouse CD8a (clone 53.6.7) antibodies were purchased from BD Pharmingen (San Diego, CA). Purified rat anti-mouse monoclonal antibodies against CD4 (clone GK1.5) and CD8 (clone 2.43) were purchased from BioXCell (West Lebanon, NH).

Seven HPV6b E7 overlapping peptides (18–30 amino acids in length and overlapping by 10 amino acids) that span the full length of E7 protein, and HPV6b E7aa21-29, E7aa28-37, E7aa31-40 and E7aa82-90 peptides were synthesized by GenScript (Piscataway, NJ). The identities of the peptides were validated by mass spectrometric analysis, and the purity of the peptides was confirmed by high-performance liquid chromatography (HPLC). FITC-conjugated rat anti-mouse IFN-γ (clone XMG1.2), PE-conjugated anti-mouse CD8a (clone 53.6.7), and PE-conjugated anti-active caspase-3 antibodies were purchased from BD Pharmingen (San Diego, CA). Histidine-rich amphipathic peptide LAH4 (sequence: KKALLALALHHLAHLALHLALALKKA) was synthesized by GenScript. CpG-ODN 1826 (CpG) was synthesized by Invitrogen (Carlsbad, CA). CFSE was purchased from BD Pharmingen.