A total of 4 × 107 spleen cells were cultured in 4 mL of RPMI1640 medium containing 10% FCS with or without 8 μg of T. halophilus for 2 days. B220+ B cells were isolated from the spleen cells using the BD IMag Cell Separation System (Becton, Dickinson and Company). Total RNAs were prepared from B cells using ISOGEN II (NIPPON GENE). The gene expression analysis was performed by DNA microarray. The measurement was entrusted to Macrogen JAPAN. DNA microarray analysis used the SurePrint G3 Mouse Gene Expression 8x60K (Agilent Technologies). Finally, the data were analyzed by the genetic manifested software R version 2.15.1.
Sureprint g3 mouse gene expression 8x60k
Manufactured by Agilent Technologies
The SurePrint G3 Mouse Gene Expression 8x60K is a microarray platform from Agilent Technologies that allows for the comprehensive analysis of mouse gene expression. The platform consists of 60,000 probes designed to target mouse genes, with eight separate microarrays on a single slide, enabling parallel processing of multiple samples.
Automatically generated - may contain errors
3 protocols using sureprint g3 mouse gene expression 8x60k
1
Transcriptome Analysis of B Cells
2
Mouse Mammary Tumor Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gene expression profiling was performed using RNA isolated from 18 snap-frozen MPA/DMBA-induced tumors and six normal/untreated mouse mammary gland samples. Whole genome expression data was obtained using Agilent Sureprint G3 Mouse Gene Expression 8x60K microarrays (Agilent Technologies cat.# G4852B) with Low Input Quick Amp Labeling protocol (Agilent Technologies cat.# 5190-2331) and the Cy3 fluorophore. Forty nanogram RNA was used for input. Microarrays were scanned using an Agilent SureScan Microarray Scanner (Agilent Technologies cat.# G4900DA), and data was extracted using Agilent Feature Extraction software. One tumor sample (S422_15_2) failed quality control and was excluded from further gene expression analyses.
3
Transcriptomic Profiling of NK Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was isolated using TRIzol reagent, and cDNA synthesis was done using M-MLV Reverse Transcriptase and random primers according to manufacturer instructions. qPCR and PCR were carried out using SYBR Premix Ex Taq and 23EasyTaq PCR SuperMix, respectively. Primers pairs for target genes are shown in Key Resources Table .
NK cells were isolated from the spleens and lungs for whole-genome transcriptome analyses using SurePrint G3 Mouse Gene Expression (8x60K) microarrays (Agilent Technologies) in duplicate. The heat map of differentially expressed genes was analyzed with MEV v4.8.1. For selecting the differentially expressed genes associated with certain biologic processes, we used the Gene Ontology (GO) project. The microarray data were uploaded to GEO (accession number: GSE103856).
NK cells were isolated from the spleens and lungs for whole-genome transcriptome analyses using SurePrint G3 Mouse Gene Expression (8x60K) microarrays (Agilent Technologies) in duplicate. The heat map of differentially expressed genes was analyzed with MEV v4.8.1. For selecting the differentially expressed genes associated with certain biologic processes, we used the Gene Ontology (GO) project. The microarray data were uploaded to GEO (accession number: GSE103856).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!