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Physcion is a laboratory equipment product manufactured by Chengdu Must Bio-Technology. It is used for the analysis and measurement of physical and chemical properties of samples. The core function of Physcion is to provide accurate and reliable data for research and testing purposes.

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4 protocols using physcion

1

Authentication and Chemical Standards for Polygonum multiflorum

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The PMR materials were collected from three different habitats in China, and three replicate sample materials were from different biological plants (Table 4). All samples were authenticated as the tuberous roots of Polygonum multiflorum Thunb. by Prof. Zhong-Zhen Zhao from the School of Chinese Medicine, Hong Kong Baptist University. Voucher specimens were deposited in the Bank of China (Hong Kong) Chinese Medicines Centre of Hong Kong Baptist University.
Chemical standards for trans-THSG, emodin, physcion, catechin, proanthocyanidin B1, proanthocyanidin B2, and gallic acid were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China); standards for cis-THSG, epicatechin, epicatechin-3-gallate, emodin-8-O-β-D-glucoside, and physcion-8-O-β-D-glucoside were purchased from Chengdu Xunchen Biological Technology Co., Ltd. (Chengdu, China). HPLC-grade methanol and acetonitrile (E. Merck, Darmstadt, Germany), ammonium acetate (Sigma-Aldrich, St. Louis, MO, USA), HPLC grade formic acid (Tedia, Fairfield, OH, USA) were purchased. Ultra-pure water was prepared by a Mili-Q water purification system (Millipore, Burlington, MA, USA).
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2

Authentication and Characterization of Polygonum multiflorum

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RPMR materials were collected from Kaili City (Guizhou, China), the geo-authentic producing area of PMR, and were authenticated as the tuberous roots of Polygonum multiflorum Thunb. by Prof. Zhongzhen Zhao. The voucher specimens were deposited in the Chinese Medicines Centre, Hong Kong Baptist University.
Chemical standards including gallic acid, proanthocyanidin B1, proanthocyanidin B2, trans-2,3,5,4′-tetrahydroxylstilbene-2-O-β-D-glucoside (trans-THSG), emodin, physcion were ordered from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China); epicatechin, epicatechin-gallate, emodin-8-O-β-D-glucoside, physcion-8-O-β-D-glucoside were supplied by Chengdu Xunchen Biological Technology Co., Ltd. (Chengdu, China). Acetonitrile (HPLC grade) and methanol (HPLC grade) were supplied by E. Merck (Darmstadt, Germany); ammounium acetate (Sigma-aldrich, USA) was purchased; formic acid (HPLC grade) was purchased from Tedia (USA); ultra-pure water was prepared by a Mili-Q water purification system (Millipore, MA, USA).
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3

Comprehensive Compound Sourcing for Research

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Ginsenoside Ro (98%), oleanolic acid (99%), Stigmasterol (95%), palmatine hydrochloride (98%), betherine (98%), epiberberine (98%), coptisine (98%), astragalin (98%), isoquercitrin (98%), baicalin (98%), wogonin (98%), chrysophanol (98%), physcion (98%) and Geniposide (98%) were sourced from the Chengdu Must Bio-technology Co., Ltd (Chengdu, Sichuan Province, China). Betaine (98%), nonanedioic acid (99.5%), succinic acid (90%), allantoin (98.5%), rutin (95%), β-sitosterol (95%) and 5-hydroxymethyl furaldehyde (99%) were sourced from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). β-ecdysterone was sourced from J&K Scientific Ltd (Beijing, China).
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4

Comprehensive Phytochemical Characterization of PMRP

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HPLC grade acetonitrile (ACN) and acetic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA), and Dikma Technology Co., Ltd. (Beijing, China). Ethanol (industrial grade) was obtained from Shandong Yuwang Industry Co., Ltd. (Shandong, China). Purified water was obtained from the Hangzhou Wahaha Corporation (Hangzhou, China). PMRP was bought from GuoDa Pharmacy (Shenyang, Liaoning, China) and was identified as the processed root of Polygonum multiflorum Thunb. by professor Zhiguo Yu of the Shenyang Pharmaceutical University. The reference standards of emodin, physcion, and gallic acid were purchased from Chengdu MUST Bio-Technology Co., Ltd. (Sichuan, China). Chrysophanol, rhein and polydatin were obtained from the National Institute for the Control of Pharmaceutical and Biological Products (NICPBP, Beijing, China). emodin-8-O-β-d-glucoside and 2,3,5,4-tetrahydroxystilbene-2-O-β-d-glucoside (THSG) were obtained from Sichuan Victory Bio-Technology Co., Ltd. (Sichuan, China). The purity of all the reference substances was higher than 98%.
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