Fasting blood glucose was determined by using the ONETOUCH Ultra 2 Blood glucose monitoring system (LIFESCAN, CHINA). Fasting blood glucose was determined after a 12-hr fast.
Onetouch ultra 2 blood glucose monitoring system
Manufactured by LifeScan
Sourced in United States
The OneTouch® Ultra®2 Blood Glucose Monitoring System is a device used for measuring blood glucose levels. It provides a way to monitor blood sugar levels through a simple testing process.
Automatically generated - may contain errors
10 protocols using onetouch ultra 2 blood glucose monitoring system
1
Fasting Glucose Measurement Protocol
2
STZ-Induced Diabetic Mice Insulin Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
12-Week old, male, DBA/2 J mice (The Jackson Laboratories, Bar Harbor, ME) received ip injections of STZ (T1D) or citrate buffer (non-T1D, n = 12) as described for the prevention study. After 4 weeks of confirmed T1D (non-fasting blood glucose > 250 mg/dl per week), an Alzet minipump was implanted subcutaneously in the left anterodorsal region of each T1D mouse (18-weeks of age) to continuously deliver either saline (n = 11 instead of 12 because 1 died within 5 weeks) or 0.25 units/day of insulin (n = 10 instead of 12 because 2 died within 5 weeks). Matched for age, non-T1D mouse group also received continuous saline via the same minipump technique (Fig. 1 ). All mice were euthanized 4 weeks after pump implantation at 22-weeks of age.
For both studies, non-fasting glucose levels were monitored weekly with a glucometer (OneTouch® Ultra®2 Blood Glucose Monitoring System, Lifescan, Inc., Milpitas, CA). Following euthanasia, the left femurs were stored in phosphate buffered saline (PBS) at − 20 °C. All animal procedures followed a protocol approved by the University of Kentucky Institutional Animal Care and Use Committee or the University of Arkansas for Medical Sciences Institutional Animal Care and Use Committee.
For both studies, non-fasting glucose levels were monitored weekly with a glucometer (OneTouch® Ultra®2 Blood Glucose Monitoring System, Lifescan, Inc., Milpitas, CA). Following euthanasia, the left femurs were stored in phosphate buffered saline (PBS) at − 20 °C. All animal procedures followed a protocol approved by the University of Kentucky Institutional Animal Care and Use Committee or the University of Arkansas for Medical Sciences Institutional Animal Care and Use Committee.
3
Glucose and Lactate Measurements by Finger Prick
4
Glucose Measurement with PGM Device
5
Comprehensive Metabolic Biomarker Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fasting or fed blood glucose (BG) was measured via glucometer (OneTouch Ultra 2 blood glucose monitoring system, Lifescan, Inc., Milpitas, CA). PINP (total procollagen type 1 N-terminal propeptide) was measured using the Rat/Mouse P1NP Competitive Enzyme Immunoassay (Immunodiagnostics Systems, Inc., Fountain Hills, AZ; #AC-33F1). RatLaps (C-terminal telopeptide I) and OPG (osteoprotegerin) were measured by rodent-specific ELISA (Immunodiagnostics Systems, Inc., Fountain Hills, AZ, AC-06F1; and RayBiotech Inc., Norcross, GA, ELM-OPG-1, respectively). Similarly, mouse osteocalcin components [carboxylated osteocalcin (cOC) and incompletely carboxylated or undercarboxylated osteocalcin (ucOC)] were measured by ELISA (MyBioSource.com, San Diego, CA; #MBS744268 and #MBS706251, respectively). Fasting insulin (measured at baseline during ipGTT), was quantified using a mouse ultrasensitive insulin ELISA (Crystal Chem USA, Elk Grove Village, IL; #90080).
6
Low-Dose STZ Induces Type 2 Diabetes in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Low dose streptozotocin (STZ) can maintain insulin secretory function and produce a diabetes state. The diabetes state models the partial and continuous loss of β cells in type 2 diabetes mellitus. The metabolic characteristics of STZ-induced diabetic mouse induced by low dose STZ are closer to those of type 2 diabetes in humans compared with the gene rodent models [16 (link), 17 (link)]. So, in the study, we chose low dose STZ for type 2 diabetes induction. Consecutive peritoneal injections of streptozotocin (STZ) (50 mg/kg/day) were utilized to induce diabetes in 14 2-month-old adult male mice for each group. Whole blood was gathered from the mouse tail vein 72 h after the last STZ injection. The data of random glucose levels were measured through the One Touch Ultra 2 blood glucose monitoring system (Life Scan Inc, CA, USA). The mice would be used for the study only if they were considered diabetic and had hyperglycemia (fasting blood glucose at least 16.7 mM) at 72 h after the STZ injections [18 (link)]. Citrate buffer-treated mice were used as the non-diabetic control. Two months after the induction of diabetes, all the mice were subjected to the following experiments.
7
Inducing and Treating Type-1 and Type-2 Diabetes in Mice
8
Doxycycline Effects on Diabetic Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male DBA/2J mice, 10–11 weeks of age, (The Jackson Laboratories, Bar Harbor, Maine) were treated either with streptozotocin (STZ) to induce diabetes (40 mg/kg/day × 5 days) or with vehicle (100 mM citrate, pH 4.5). At ~ 12–13 weeks of age, diabetic mice, as confirmed by glucosuria, were then randomly assigned to treatment with doxycycline (DOX) rodent chow (STZ + DOX: Bio-Serv product #S3888, doxycycline concentration of 200 mg/kg, Frenchtown, NJ; n = 20) or with a control (CON) chow for doxycycline diets (STZ + CON: Bio-Serv product #S4207; n = 20). Vehicle-treated (non-diabetic) mice were similarly fed, receiving either doxycycline chow (VEH + DOX; n = 10) or control chow (VEH + CON; n = 10). Mice were provided ad libitum access to water and to their assigned food. Food consumption and body weight (Table 1 ) were measured weekly for the next 10 weeks. Blood glucose was measured on trunk blood at sacrifice via glucometer (OneTouch® Ultra®2 Blood Glucose Monitoring System, LifeScan, Inc., Milpitas, CA; average intra-assay coefficient of variation of 1.7% across a range of 40–300 mg/dl target glucose concentrations). All procedures were approved by the Institutional Animal Care and Use Committee at the University of Arkansas for Medical Sciences.
9
Doxycycline Effects in Diabetic Mice
10
Weekly Blood Glucose and Serum Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood glucose concentrations were measured and recorded weekly using a One Touch Ultra 2 Blood Glucose Monitoring System (Lifescan Canada Ltd, Burnaby, BC, Canada) and One Touch test strips (Lifescan Canada Ltd). Serum was collected at the completion of the study prior to dissection via heart puncture; ET-1 (ADI-900-020A; Enzo Life Sciences, Farmingdale, NY, USA) and insulin concentrations (Catalogue no. 80-INSHU-E01.1; ALPCO, Salem, NH, USA) were determined via enzyme-linked immunosorbent assay (ELISA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!