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Recombinant flag gpnmb

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Recombinant Flag-GPNMB is a laboratory reagent produced by OriGene. It is a fusion protein composed of the GPNMB protein and a Flag tag. GPNMB is a transmembrane glycoprotein that functions in diverse cellular processes. The Flag tag is a short peptide sequence that can be used for detection and purification of the recombinant protein.

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Lab products found in correlation

Transcend non radioactive translation detection system, by Promega (2 mentions) Tnt quick coupled transcription translation kit, by Promega (2 mentions) Lrrk2, by Sino Biological (2 mentions) Gst egfr, by Active Motif (2 mentions) Recombinant active caspase 1, by R&D Systems (2 mentions) Protein purification kit, by Takara Bio (2 mentions) Bl21 codonplus de3 ripl, by Agilent Technologies (2 mentions) Hif 1α, by Novus Biologicals (2 mentions)

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GPNMB (2 citations)

2 protocols using recombinant flag gpnmb

1

Recombinant Protein Expression and Purification

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Recombinant proteins were expressed in E.coli strain BL21-CodonPlus® (DE3)-RIPL (Agilent Technologies) and purified using Protein Purification Kit (Clontech). Recombinant Flag-GPNMB and PHD1 were purchased from Origene. GST-EGFR was purchased from Active Motif, HIF1α and BRK were purchased from Novus Biologicals. LRRK2 was purchased from SignalChem. Recombinant active Caspase-1 was purchased from R&D Systems. In vitro translation of LINK-A was conducted using TnT® Quick Coupled Transcription/Translation Kit and detection was performed using Transcend™ Non-Radioactive Translation Detection System (Promega).
Lin A., Li C., Xing Z., Hu Q., Liang K., Han L., Wang C., Hawke D.H., Wang S., Zhang Y., Wei Y., Ma G., Park P.K., Zhou J., Zhou Y., Hu Z., Zhou Y., Marks J.R., Liang H., Hung M.C., Lin C, & Yang L. (2016). The LINK-A lncRNA Activates Normoxic HIF1α Signaling in Triple-negative Breast Cancer. Nature cell biology, 18(2), 213-224.
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2

Recombinant Protein Expression and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant proteins were expressed in E.coli strain BL21-CodonPlus® (DE3)-RIPL (Agilent Technologies) and purified using Protein Purification Kit (Clontech). Recombinant Flag-GPNMB and PHD1 were purchased from Origene. GST-EGFR was purchased from Active Motif, HIF1α and BRK were purchased from Novus Biologicals. LRRK2 was purchased from SignalChem. Recombinant active Caspase-1 was purchased from R&D Systems. In vitro translation of LINK-A was conducted using TnT® Quick Coupled Transcription/Translation Kit and detection was performed using Transcend™ Non-Radioactive Translation Detection System (Promega).
Lin A., Li C., Xing Z., Hu Q., Liang K., Han L., Wang C., Hawke D.H., Wang S., Zhang Y., Wei Y., Ma G., Park P.K., Zhou J., Zhou Y., Hu Z., Zhou Y., Marks J.R., Liang H., Hung M.C., Lin C, & Yang L. (2016). The LINK-A lncRNA Activates Normoxic HIF1α Signaling in Triple-negative Breast Cancer. Nature cell biology, 18(2), 213-224.
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