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Cd3 pe cy5 clone hit3a

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The CD3 PE-Cy5 (clone HIT3a) is a flow cytometry antibody that binds to the CD3 complex on the surface of T cells. It is used to identify and enumerate T cells in a sample.

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Lab products found in correlation

Facsaria 2, by BD (1 mentions) Aqua vital dye, by Thermo Fisher Scientific (1 mentions) Igd pe clone ia6 2, by BD (1 mentions) Cd27 pe cy7 clone o323, by Thermo Fisher Scientific (1 mentions) Cd69 pe clone fn50, by BD (1 mentions)

Spelling variants of this product

CD3-PE (103 citations) HIT3a (18 citations) Clone HIT3a (5 citations) CD3 (clone HIT3a), (4 citations) PE-Cy5-CD3 (2 citations)

2 protocols using cd3 pe cy5 clone hit3a

1

ZIKV-Reactive B Cell Profiling

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Thawed PBMCs were stained with Aqua Vital Dye (Invitrogen), IgD-PE (clone IA6-2; BD Biosciences), CD10 PE-CF594 (clone HI10a; BD Biosciences; CD3 PE-Cy5 (clone HIT3a; BD Biosciences), CD14 BV605 (clone M5E2; BD Biolegend), CD16 BV570 (clone 3G8; Biolegend), CD27 PE-Cy7 (clone O323; Thermo Fisher Scientific), CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1; BD Biosciences) and 1 x 106 PFU of freshly thawed UV-inactivated ZIKV labelled with AF488. Additionally, 5 μM of Chk2 kinase inhibitor II (Calbiochem/EMD Chemicals) was added to prevent cell death. Unfractionated B cells were defined as CD14−/CD16−/CD3−/CD19+. For memory B cells an additional IgD−/CD27all gate was applied. The ZIKV-reactive AF488 gate was set using a fluorescence minus one condition. Cells were sorted on a Beckton Dickinson FACS Aria II and analysis was performed in FlowJo.
Singh T., Hwang K.K., Miller A.S., Jones R.L., Lopez C.A., Dulson S.J., Giuberti C., Gladden M.A., Miller I., Webster H.S., Eudailey J.A., Luo K., Von Holle T., Edwards R.J., Valencia S., Burgomaster K.E., Zhang S., Mangold J.F., Tu J.J., Dennis M., Alam S.M., Premkumar L., Dietze R., Pierson T.C., Eong Ooi E., Lazear H.M., Kuhn R.J., Permar S.R, & Bonsignori M. (2022). A Zika virus-specific IgM elicited in pregnancy exhibits ultrapotent neutralization. Cell, 185(25), 4826-4840.e17.
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2

Assessing CD69 Expression on iNKT Cells

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For the assessment of CD69 expression on iNKT cells, PB mononuclear cells were incubated with the following MoAbs: TCR Vα24-Jα18 FITC (clone 6B11; BD Biosciences; cat. no. 558371; 20 m/test), CD3 PE-Cy5 (clone HIT3a; BD Biosciences; cat. no. 555341; 20 µl/test) and CD69 PE (clone FN50; BD Biosciences; cat. no. 555531, 20 µl/test). Samples were analyzed using flow cytometry immediately following preparation.
Bojarska-Junak A., Waldowska M., Woś J., Chocholska S., Hus I., Tomczak W., Dzik M., Hus M, & Roliński J. (2017). Intracellular IL-4 and IFN-γ expression in iNKT cells from patients with chronic lymphocytic leukemia. Oncology Letters, 15(2), 1580-1590.
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