Optima grade acetonitrile

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

Optima grade acetonitrile is a high-purity solvent used in various analytical and chemical applications. It is a colorless, volatile, and flammable liquid with a characteristic odor. Optima grade acetonitrile meets strict quality standards and is suitable for use in sensitive analytical techniques, such as high-performance liquid chromatography (HPLC), gas chromatography (GC), and mass spectrometry (MS).

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Acetonitrile (2707 citations) Optima grade (92 citations) Optima LC/MS grade acetonitrile (49 citations) Optima LC/MS grade acetonitrile and water (4 citations) Fisher Optima grade acetonitrile (3 citations) Optima™ LC–MS grade acetonitrile (ACN), (2 citations) Optima HPLC grade acetonitrile (2 citations) Optima-grade acetonitrile (ACN) (2 citations)

23 protocols using optima grade acetonitrile

Synthesis and Characterization of Rhenium Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rhenium(I) pentacarbonyl chloride, silver
trifluoromethanesulfonate, and IR-grade potassium bromide were purchased
from Aldrich. Diethyl ether, HPLC-grade methanol, and optima-grade
acetonitrile were purchased from Fisher Scientific. Absolute ethanol
was obtained from AAPER. The solvents, methanol, hexanes, and acetonitrile
were obtained from Fisher. The compound 1,10-phenanthroline (phen)
was obtained from GFS. Deuterated solvents, CDCl₃, CD₃OD, and (CD₃)₂S=O, were obtained
from Cambridge Isotopes. Diethyl ether, HPLC-grade methanol, methylene
chloride, hexanes, and optima-grade acetonitrile for UV–visible
spectral determinations were purchased from Fisher Scientific. Acetonitrile
was used as solvent for absorption and emission studies. Electrochemical
grade tetrabutylammonium hexafluorophosphate was purchased from Southwestern
Analytical. A ferrocene standard used in electrochemical studies was
purchased from Aldrich. Dried acetonitrile used as a electrochemical
background was contained in a Sure/Seal bottle and was purchased from
Aldrich.

Stoyanov S.R., Komreddy V., Rillema D.P., Moore C.E, & Nguyen H. (2020). Synthesis and Computational and Experimental Investigations of a para-Nicotinic Acid-Bridged Dirhenium(I) Dimer Complex. ACS Omega, 5(22), 12944-12954.

+ Open protocol

+ Expand

Quantitative Mass Spectrometry Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols

Optima grade water + 0.1% formic acid, Optima grade acetonitrile +0.1% formic acid, L-ascorbic acid, hydroquinone, and acetone were purchased from Fisher Scientific (Fair Lawn, NJ). All chemicals were ACS reagent grade or higher. MassPREP™ Digestion Standard Protein Expression Mixture 2 and enolase digestion standard were obtained from Waters Corporation (Milford, MA).

Grinias K.M., Godinho J.M., Franklin E.G., Stobaugh J.T, & Jorgenson J.W. (2016). Development of a 45 kpsi Ultrahigh Pressure Liquid Chromatography Instrument for Gradient Separations of Peptides Using Long Microcapillary Columns and Sub-2 µm Particles. Journal of chromatography. A, 1469, 60-67.

+ Open protocol

+ Expand

Quantifying Eicosanoid Metabolites via Liquid Chromatography-Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Stocks of AA, cis-EETs (14,15-, 11,12-, 8,9-, and 5,6-EETs), deuterated internal standards (14,15-EET-d₁₁, 8,9-EET-d₁₁, 5,6-EET-d₁₁, and 14,15-DHET-d₁₁) and 4-[[trans-4-[[(tricyclo[3.3.1.1^3,7]dec-1-ylamino)carbonyl]amino]cyclohexyl]oxy]-benzoic acid (t-AUCB) were purchased from Cayman Chemical (Ann Arbor, MI). 2,2′-azobis(4-methoxy-2,4-dimethylvaleronitrile) (MeOAMVN) and 2,2′-azobis(2-(2-imidazolin-2-yl) propane) dihydrochloride (AIPH) were obtained from Wako Chemicals USA, Inc (Richmond, VA). Pure AA stocks were purchased from Nu-Chek-Prep, Inc (Elysian, MN). Dulbecco's phosphate buffered saline (PBS), ACS-grade ethyl acetate, chloroform, Optima-grade acetonitrile, water, and methanol were purchased from Fisher Scientific (Hampton, NH). Benzene, triphenylphosphine (TPP), butylated hydroxytoluene (BHT), phospholipase A₂ from Naja mossambica mossambica, pyruvic acid, and diethylenetriaminepentaacetic acid (DETAPAC) were obtained from Sigma-Aldrich (St. Louis, MO). 1,2-dilauryl-sn-glycero-3-phosphocholine (DLPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were purchased from Avanti Polar Lipids, Inc (Alabaster, AL).

Aliwarga T., Raccor B.S., Lemaitre R.N., Sotoodehnia N., Gharib S.A., Xu L, & Totah R.A. (2017). Enzymatic and Free Radical Formation of Cis- and Trans- Epoxyeicosatrienoic Acids In Vitro and In Vivo. Free radical biology & medicine, 112, 131-140.

+ Open protocol

+ Expand

Dapivirine Quantification in Breast Milk

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dapivirine (TMC120 (4-[[4-[(2,4,6-trimethylphenyl)-amino]-2-pyrimidinyl]amino]benzonitrile)) was purchased from Toronto Research Chemicals (North York, ON, Canada) in powdered form. The deuterated internal standard ²H₄-dapivirine was obtained from the International Partnership for Microbicides (IPM; Silver Spring, MD, USA) in powdered form (Figure 1). Drug-free human breast milk was purchased from BioIVT (Westbury, NY, USA). Optima-grade acetonitrile, methanol, water, 0.1% formic acid in water and certified ACS plus grade ammonium hydroxide were purchased from Fisher Scientific (Fairlawn, NJ, USA). ACS reagent grade formic acid was purchased from Sigma Aldrich (St. Louis, MO, USA). HPLC grade n-Hexane was purchased from Alfa Aesar (Haverhill, MA, USA).

Manohar M, & Marzinke M.A. (2019). Validation and implementation of an ultrasensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) assay for dapivirine quantitation in breast milk. Clinical biochemistry, 82, 66-72.

+ Open protocol

+ Expand

Quantitative Bile Acid Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Optima grade acetonitrile and water were purchased from Fisher Scientific (Loughborough, UK). Ammonium acetate was purchased from Sigma-Aldrich (Dorset, UK). Formic acid was purchased from Fluka (Buchs, Switzerland). Bile acid standards, including tauro-alpha-muricholic aicd (T-α-MCA), tauro-beta-muricholic acid (T-β-MCA), tauro-omega-muricholic acid (T-ω-MCA), alpha-muricholic acid (α-MCA), beta-muricholic acid (β-MCA), tauro hyocholic acid (THCA), glyco urso deoxycholic acid (GUDCA), tauro urso deoxycholic acid (TUDCA), hyocholic acid (HCA), taurohyoxy deoxycholic acid (THDCA), taurocholic acid (TCA), cholic acid (CA), urso deoxycholic acid (UDCA), hyo deoxycholic acid (HDCA), glyco deoxycholic acid (GDCA), tauro deoxycholic acid (TDCA), cheno deoxycholic acid (CDCA), and deoxycholic acid (DCA) were purchased from Steraloids, Inc. (Newport, RI). Standard solutions of these 20 bile acids were prepared at concentrations of 0.5, 10, 50, 100, 500, 1500, 5000, and 10 000 nM in a solution of 20% (v/v) acetonitrile and water.

Poland J.C., Schrimpe-Rutledge A.C., Sherrod S.D., Flynn C.R, & McLean J.A. (2019). Utilizing Untargeted Ion Mobility-Mass Spectrometry To Profile Changes in the Gut Metabolome Following Biliary Diversion Surgery. Analytical chemistry, 91(22), 14417-14423.

+ Open protocol

+ Expand

Quantification of Bupropion Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bupropion, R-Bupropion, S-Bupropion, OH-Bupropion, R,R-OH-Bupropion, S,S-OH-Bupropion, threohydroBupropion, erythrohydroBupropion, Bupropion-d₉, OH-Bupropion-d₆, and threohydroBupropion-d₉ were purchased from Toronto Research Chemicals (Toronto, Canada). 4′-OH-Bupropion, threo-4′-OH-hydroBupropion, and erythro-4′-OH-hydroBupropion were synthesized as previously described (Sager et al., 2016 ). Hydrochloric acid, formic acid, and sodium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO). Optima grade acetonitrile, methanol, water, and pooled human liver microsomes (Gibco, mixed gender, n = 50) were purchased from Fisher Scientific (Waltham, MA). Pooled S9 fractions (n = 20, mixed gender) and recombinant P450 enzymes coexpressed with cytochrome P450 reductase and cytochrome b5 were purchased from BD Biosciences (San Jose, CA). CYP2B6 inhibitory monoclonal antibody (MAB-CYP2B6) was purchased from Corning (Corning, NY).

Sager J.E., Price L.S, & Isoherranen N. (2016). Stereoselective Metabolism of Bupropion to OH-bupropion, Threohydrobupropion, Erythrohydrobupropion, and 4′-OH-bupropion in vitro. Drug Metabolism and Disposition, 44(10), 1709-1719.

+ Open protocol

+ Expand

Analytical-Grade Chemical Standards Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemical standards used in this study were minimally analytical grade and obtained from commercial sources (Table S1). Optima grade acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ) for the mobile phase and standard solutions. Optima LC/MS ammonium acetate and formic acid and TraceMetal grade ammonium hydroxide were used as mobile phase additives and also obtained from Fisher Scientific. All water used in this study was deionized and filtered (18.2MΩ, 0.22μm).

Rhoades S.D, & Weljie A.M. (2016). Comprehensive Optimization of LC-MS Metabolomics Methods Using Design of Experiments (COLMeD). Metabolomics : Official journal of the Metabolomic Society, 12(12), 183.

+ Open protocol

+ Expand

Mass spectrometry protocol for lipid extraction

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Solvents and buffers for mass spectrometry analysis and lipid extraction were purchased from Merck (hyper grade methanol, gradient grade ethanol, LC grade MTBE, and LC-MS grade formic acid) or Fisher (optima grade acetonitrile, and 2-propanol). 1,2-diphytanoyl-sn-glycero-3-phosphocholine and 1,2-diarachidoyl-sn-glycero-3-phosphocholine were purchased from Avanti Polar Lipids (Alabama, United States), beta-L-gulopyranosyl-caldarchaetidyl-glycerol was purchased from Matreya, LLC (Pennsylvania, United States), and C46 GTGT was purchased from Pandion Laboratories LLC (Indiana, United States). Other reagents used for cell culturing and quantitative PCR assays have been described in our previous report (Law et al., 2021 (link)).

Law K.P., He W., Tao J, & Zhang C. (2021). A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry. Frontiers in Microbiology, 12, 735878.

+ Open protocol

+ Expand

Quantitative Analysis of Tamoxifen Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tamoxifen, Tamoxifen-d5, 4-hydroxy-Tamoxifen (pure Z-trans-isomer, >98 % Z), 4-hydroxy-Tamoxifen-d5, N-desmethyl-Tamoxifen hydrochloride, N-desmethyl-Tamoxifen-d5, N-desmethyl-4-hydroxy-Tamoxifen (1:1 E/Z mixture) and N-desmethyl-4-hydroxy-Tamoxifen-d5 (1:1 E/Z mixture) were obtained from Toronto Research Chemicals (North York, ON, Canada). Optima-grade acetonitrile, methanol, water and certified ACS xylene were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Formic acid and ammonium acetate were from Sigma-Aldrich (Oakville, ON, Canada). Bond Elut^® C2 (100 mg, 3 mL) solid-phase extraction (SPE) cartridges were obtained from Agilent Technologies (Santa Clara, CA, USA).

Ng E.S., Kangarloo S.B., Konno M., Paterson A, & Magliocco A.M. (2014). Extraction of tamoxifen and its metabolites from formalin-fixed, paraffin-embedded tissues: an innovative quantitation method using liquid chromatography and tandem mass spectrometry. Cancer Chemotherapy and Pharmacology, 73(3), 475-484.

+ Open protocol

+ Expand

Bile Acid Profiling with LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Optima grade acetonitrile and water were purchased from Fisher Scientific (Loughborough, UK). Ammonium acetate was purchased from Sigma-Aldrich (Dorset, UK). Formic acid was purchased from Fluka (Buchs, Switzerland). Bile acid standards were purchased from Steraloids, Inc. (Newport, RI) and included tauro-alpha-muricholic acid (T-α-MCA), tauro-beta-muricholic acid (T-β-MCA), tauro-omega-muricholic acid (T-ω-MCA), alpha-muricholic acid (α-MCA), beta-muricholic acid (β-MCA), tauro hyocholic acid (THCA), glyco ursodeoxycholic acid (GUDCA), tauro ursodeoxycholic acid (TUDCA), hyocholic acid (HCA), taurohyoxydeoxycholic acid (THDCA), taurocholic acid (TCA), cholic acid (CA), ursodeoxycholic acid (UDCA), hyodeoxycholic acid (HDCA), glycodeoxycholic acid (GDCA), taurodeoxycholic acid (TDCA), chenodeoxycholic acid (CDCA), and deoxycholic acid (DCA).

Poland J.C., Leaptrot K.L., Sherrod S.D., Flynn C.R, & McLean J.A. (2020). Collision Cross Section Conformational Analyses of Bile Acids via Ion Mobility–Mass Spectrometry. Journal of the American Society for Mass Spectrometry.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!