N butylchloride

Epothilone B was purchased from Toronto Research Chemical (Toronto, Ontario, Canada) and epothilone D was purchased from Austin Chemical (Buffalo Grove, Illinois). Dichloromethane, ferric chloride, phenylmethylsulfonyl fluoride (PMSF), cremaphor, dithiothreitol (DTT), anhydrous Dichloromethane (DCM), anhydrous N,N-dimethylformamide (DMF), N-methylmorpholine (NMM), 4-dimethylaminopyridine (DMAP), and lithium hydroxide monohydrate (LiOH·H₂O) were purchased from Sigma-Aldrich (St. Louis, Missouri). Trehalose was purchased from Carbosynth (Berkshire, United Kingdom). Dialysis tubing with 3500 molecular weight cut-off (MWC) and tangential flow filtration filters with 10 kDa pore size were purchased from Spectrum Labs (Rancho Dominguez, California). Pass-through sterile 0.22 micron filters were purchased from Sartorious (Goettingen, Germany). Thermo Syncronis C18 HPLC column (97105-254630), HPLC grade methanol, HPLC grade acetonitrile, n-butyl chloride, absolute ethanol, K₂EDTA tubes, black wall, clear bottom 96-well plates, tetrahydrofuran (THF), isopropyl alcohol, methyl tert-butyl ether (MTBE), acetic anhydride (Ac₂O), acetone, and acetic acid were purchased from Fisher Scientific (Waltham, Massachusetts). absolute ethanol was purchased from Decon Labs. Hydroxylamine (50% aqueous solution) was obtained from Alfa Aesar (Haverhill, Massachusetts).