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Anti cd71 fitc

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Anti CD71-FITC is a fluorescently-labeled antibody that binds to the CD71 (transferrin receptor) protein expressed on the surface of cells. It is used for the detection and analysis of CD71-positive cells in flow cytometry applications.

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Lab products found in correlation

Cd34 apc, by Beckman Coulter (7 mentions) Anti cd41 fitc gpiib iiia, by BD (7 mentions) Anti cd33 fitc, by Beckman Coulter (7 mentions) Anti cd41 pe, by Beckman Coulter (7 mentions) Anti cd45 percp, by Beckman Coulter (7 mentions) Facscalibur, by BD (7 mentions) Anti cd42b pe, by Beckman Coulter (2 mentions) Propidium iodide, by Merck Group (1 mentions)

9 protocols using anti cd71 fitc

1

Multiparametric Analysis of Platelet Differentiation

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Example 34

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10^6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US10253296B2. Synthetic membrane-receiver complexes (2019-04-09). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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2

Micronuclei Quantification in Reticulocytes

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To evaluate the micronucleated reticulocytes (MN-RET) in the peripheral blood, we followed the procedure described by Dertinger SD [9 (link)].
Briefly, the cell suspension in heparin was fixed with ultracold methanol (IT. Baker) and kept at − 75 °C until the day of flow cytometric analysis.
On the day of analysis, the blood samples were removed from the − 75 °C freezer and washed with ice cold bicarbonate-buffered saline solution; then, the samples were labeled with the monoclonal antibodies anti-CD71 FITC and anti-CD42b-PE (both from Beckman Coulter) and treated with RNase (Sigma). The monoclonal anti-CD42b was incorporated into the procedure to exclude platelets, which can interfere with the analysis. After an adequate incubation period (20 min in the dark at room temperature), 1 ml ice cold propidium iodide solution (1.25 mg PI (Sigma)/ml bicarbonate-buffered saline) was added, and the results were evaluated by using a flow cytometer.
Each sample was stained and analyzed in triplicate. The MN-RETs were identified as the fraction of erythrocytes negative for CD42b and positive for CD71 and propidium iodide.
Toro A., Parrinello N.L., Schembari E., Mannino M., Corsale G., Triolo A., Palermo F., Romano A., Di Raimondo F, & Di Carlo I. (2020). Single segment of spleen autotransplantation, after splenectomy for trauma, can restore splenic functions. World Journal of Emergency Surgery : WJES, 15, 17.
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3

Assessing Platelet Differentiation by Flow Cytometry

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Example 44

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10{circumflex over ( )}6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 ug/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US11554141B2. Methods and compositions for immunomodulation (2023-01-17). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Jordi Mata-Fink [US], John Round [US], Noubar B. Afeyan [US], Avak Kahvejian [US].
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4

Assessing Platelet Differentiation by Flow Cytometry

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Example 34

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10{circumflex over ( )}6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US10557119B2. Erythroid cells comprising phenylalanine ammonia lyase (2020-02-11). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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5

Assessing Platelet Differentiation by Flow Cytometry

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Example 34

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10{circumflex over ( )}6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US10344263B2. Synthetic membrane-receiver complexes (2019-07-09). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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6

Multiparametric Analysis of Platelet Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 34

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10^6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US10301594B1. Synthetic membrane-receiver complexes (2019-05-28). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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7

Quantifying Micronucleated Reticulocytes by Flow Cytometry

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Dertinger SD is used to quantify or assess micronucleated reticulocytes. For each analysis [9 (link)], The samples were blended with anti-CD71 FITC and anti-CD42b-PE (monoclonal antibodies) (both from Beckman Coulter), then bicarbonate-buffered used to be as soon as used to wash it with saline solution, accompanied through the use of RNase treatment. Ultracold methanol (IT. Baker) is constantly combined with heparin and saved at 75 °C (Sigma). The remedy blanketed the monoclonal anti-CD42b. to now no longer perform platelets. After 20 min barring light, we add 1 ml of ice-cold propidium iodide reply (1.25 mg PI (Sigma)/ml bicarbonate-buffered saline). MN-RETs confirmed RBCs that had been top-notch for CD71, on the other hand, bad for CD42b.
Triple examination and staining of the sample. The erythrocyte fraction was MN-RET, negative for CD42b but positive for CD71 and propidium iodide.
Saad H.A., Elsayed R.S., Riad M., El-Taher A.K., Eraky M.E, & Abdelmonem A. (2023). Revitalize splenic functions. Following a splenectomy for trauma, a small amount of splenic autotransplantation was performed. BMC Surgery, 23, 230.
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8

Platelet Differentiation Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 44

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10{circumflex over ( )}6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US11576934B2. Methods and compositions for immunomodulation (2023-02-14). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Jordi Mata-Fink [US], John Round [US], Noubar B. Afeyan [US], Avak Kahvejian [US].
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9

Assessing Platelet Differentiation and Maturation

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Example 34

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10^6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US10329531B2. Synthetic membrane-receiver complexes (2019-06-25). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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