Rabbit anti calbindin

Manufactured by Swant

Sourced in Switzerland

Rabbit anti-calbindin is a primary antibody that targets the calbindin protein, which is a calcium-binding protein found in various cell types. This antibody can be used in immunohistochemistry, western blotting, and other applications to detect and study the distribution and expression of calbindin in biological samples.

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Lab products found in correlation

Rabbit anti gfp, by Thermo Fisher Scientific (5 mentions) Goat anti chat, by Merck Group (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions) Mouse anti gad67, by Merck Group (3 mentions) Rabbit anti iba1, by Fujifilm (3 mentions) Mouse anti neun, by Merck Group (3 mentions) Rabbit anti gfap, by Agilent Technologies (3 mentions) Mouse anti brn3a, by Merck Group (2 mentions) Mouse anti calbindin, by Merck Group (2 mentions) Rabbit anti calretinin, by Swant (2 mentions) Rabbit anti dsred, by Takara Bio (2 mentions) Rabbit anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Santa Cruz Biotechnology (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti gfap, by Synaptic Systems (1 mentions) Mouse anti pkcα, by BD (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Abc kit, by Vector Laboratories (1 mentions) Mouse anti calretinin, by Swant (1 mentions) Rabbit anti parvalbumin, by Swant (1 mentions) Goat anti brn3, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Calbindin (27 citations) Rabbit anti-calbindin d28k (12 citations) Anti-calbindin (9 citations)

22 protocols using rabbit anti calbindin

Diverse Antibody Characterization Protocol

Cited 1 time

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Antibodies used in the study were goat anti-choline acetyltransferase (ChAT, cat# AB144P) and rabbit anti-MEGF10 (cat# ABC10) from Millipore; rabbit anti-calbindin (cat# 300) from Swant; rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH, cat# SC25778) from Santa Cruz Biotechnology; mouse anti-CPEB3 and affinity-purified polyclonal rabbit anit-CPEB3 (homemade; Chao et al., 2012 (link); Wang and Huang, 2012 (link)). Alexa Fluor-conjugated secondary antibodies were from Invitrogen.

Chen Y.P., Bai G.S., Wu M.F., Chiao C.C, & Huang Y.S. (2016). Loss of CPEB3 Upregulates MEGF10 to Impair Mosaic Development of ON Starburst Amacrine Cells. Frontiers in Molecular Neuroscience, 9, 105.

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Immunohistochemical Staining of Brain Markers

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The following antibodies were used: Monoclonal mouse anti-Bassoon mab7f (1∶2,500; Stressgen, MI, USA), mouse anti-GFAP (1∶500; Synaptic Systems, Göttingen, Germany), mouse anti-PKCα (1∶20,000; BD Biosciences, Heidelberg, Germany), polyclonal rabbit anti-Cacna1f (Pep3; 1∶3,000; [15] (link)), rabbit anti-ubMunc13-2 (1∶6,000; [18] (link)), rabbit anti-Calbindin (1∶1,000; Swant, Marly, Switzerland), polyclonal guinea pig anti-Pclo 44a (1∶4,000; [19] (link)), guinea pig anti-VGluT1 (1∶50,000; Millipore, Billerica, MA, USA). DAPI was used to stain nuclei (1∶20,000; Sigma, Taufkirchen, Germany).

Regus-Leidig H., Atorf J., Feigenspan A., Kremers J., Maw M.A, & Brandstätter J.H. (2014). Photoreceptor Degeneration in Two Mouse Models for Congenital Stationary Night Blindness Type 2. PLoS ONE, 9(1), e86769.

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Immunostaining of Brain Sections

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Tissue processing and immunostaining were performed on free-floating sections (40 µm) following standard published techniques.^{13 (link)} Coronal brain sections were incubated overnight with either rabbit anticalbindin (1:15 000–1:100 000; Swant), biotinylated rabbit anti-3D6 (1:3500; Elan Pharmaceuticals), or mouse antisynaptophysin (1:1000) primary antibodies. Calbindin and 3D6 staining was revealed using the ABC kit (Vector) with diaminobenzidine (Sigma-Aldrich). Mean signal intensity was measured using National Institutes of Health ImageJ software. Synaptophysin was labeled with a fluorescent secondary antibody and quantified as previously reported.^{14 (link)}

Middeldorp J., Lehallier B., Villeda S.A., Miedema S.S., Evans E., Czirr E., Zhang H., Luo J., Stan T., Mosher K.I., Masliah E, & Wyss-Coray T. (2016). Preclinical Assessment of Young Blood Plasma for Alzheimer Disease. JAMA neurology, 73(11), 1325-1333.

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Immunohistochemical Analysis of Retinal Cells

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After electrophysiological experiments, some retinas were fixed in fresh 4% paraformaldehyde in PBS at 4 degrees C for 1 hour. After fixation, the retinas were washed and incubated at 4 degrees C with primary antibodies for 4–5 days. Secondary antibody incubation at room temperature for at least 2 hours preceded mounting on a glass slide with spacers, ganglion cell side up, with Prolong Gold (Invitrogen). Whole mount images were obtained on a LSM 710 inverted NLO microscope at 20X or 40X (Zeiss). The primary antibodies used were: anti-GFP (rabbit, Life Technologies; chick, Abcam); mouse anti-Nonphosphoneurofilament H (SMI-32, Covance); goat anti-Osteopontin (R&D Systems); rabbit anti-Parvalbumin, rabbit anti-Calbindin, and mouse anti-Calretinin (all from Swant); anti-vAChT (goat, Promega; guinea pig, Millipore); goat anti-ChAT (Millipore); mouse anti-Brn3a (Millipore); goat anti-Brn3 (raised against Brn3b, Santa Cruz Biotechnology) and mouse anti-Brn3c (Santa Cruz Biotechnology). Dylight405-, Alexa488-, Cy3- and Alexa647-conjugated secondary antibodies were obtained from Jackson Immunoresearch.

Krieger B., Qiao M., Rousso D.L., Sanes J.R, & Meister M. (2017). Four alpha ganglion cell types in mouse retina: Function, structure, and molecular signatures. PLoS ONE, 12(7), e0180091.

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TUNEL Assay and Calbindin Immunostaining

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TUNEL assay was performed as previously described (Kirtay et al., 2021 (link)). After the Tunel staining, sections were incubated by the first antibody rabbit anti-Calbindin (1:200, Swant) at 4°C overnight. After washing with PBS 3 times, sections were incubated by secondary antibody (1:200, Sigma) together with DAPI (1:5,000, Invitrogen) at room temperature for 1.5 h. Images were captured by the ApoTome microscope (Zeiss Jena, Germany) under 40 × objectives.

Ding M., Qing X., Zhang G., Baade-Büttner C., Gruber R., Lu H., Ferguson D.O., Geis C., Wang Z.Q, & Zhou Z.W. (2022). The Essential DNA Damage Response Complex MRN Is Dispensable for the Survival and Function of Purkinje Neurons. Frontiers in Aging Neuroscience, 13, 786199.

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Immunohistochemical Antibody Panel

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Antibodies used in these studies were as follows: rabbit anti-calbindin (diluted 1:2500 for immunohistochemistry (IHC); Swant), rabbit anti-calretinin (diluted 1:1000 for IHC; Millipore), rabbit anti-GFP (diluted 1:500; Life Technologies), mouse anti-GAD67 (diluted 1:1000 for IHC; Millipore), rabbit anti-Iba1 (diluted 1:500 for IHC, WAKO), rabbit anti-Vasoactive Intestinal Peptide (VIP; diluted 1:150 for IHC; Immunostar). All fluorescent secondary antibodies for IHC were from Life Technologies (diluted 1:1000).

Monavarfeshani A., Knill C.N., Sabbagh U., Su J, & Fox M.A. (2017). Region- and Cell-Specific Expression of Transmembrane Collagens in Mouse Brain. Frontiers in Integrative Neuroscience, 11, 20.

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Antibodies and Stains for Neurological Research

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The following antibodies and stains were used in this study: Dapi (Cell Signaling Technology, 1:50,000), PNA-488 and PNA-647 (Invitrogen, 1:1000), mouse anti-PSD95 (Neuromabs, 1:400), rabbit anti-HCN4 (Alomone labs, 1:500), rabbit anti-NK3R (Novus, 1:2,000), rabbit anti-recoverin (Millipore, 1:500), mouse anti-PKARIIβ (BD transduction laboratories, 1:500), mouse anti-Syt2 (ZNP1) (ZFIN, 1:200), mouse anti-PKCα (SCBT, 1:500), rabbit anti-PKCα (SCBT, 1:500), rabbit anti-calbindin (Swant, 1:200), mouse anti-β-catenin (BD transduction laboratories, 1:500), rat anti-α-n-catenin (DSHB, 1:200), rabbit anti-Magi 2 (Sigma, 1:500), goat anti-blue cone opsin (Santa Cruz Biotechnology 1:500), mouse anti-γ-protocadherin (Neuromabs, 1:400), rabbit anti-GS (BD transduction labs, 1:1,000), mouse anti-cadherin-8 (DSHB, 1:100), mouse anti-CASK (Neuromabs, 1:400), rat anti-r-cadherin (DSHB, 1:50). Secondary antibodies used in this study were purchased from Jackson Immuno Research, conjugated to alexa 488, cy3 or dylight 647 and used at a concentration of 1:500.

Nuhn J.S, & Fuerst P.G. (2014). Developmental Localization of Adhesion and Scaffolding Proteins at the Cone Synapse. Gene expression patterns : GEP, 16(1), 36-50.

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Immunohistochemical Markers for Cellular Analysis

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Primary antibodies (supplier; dilutions) used in this study were as follows: rabbit anti-Calbindin (Swant; 1∶10,000); goat anti-ChAT (Millipore; 1∶500); rabbit anti-GFAP (DAKO; 1∶8,000); mouse anti-GM130 (BD Transduction; 1∶100); rabbit anti-Hsp25 (Enzo; 1∶8,000); rabbit anti-Iba-1 (Wako; 1∶5,000); rat anti-Ki67 (DAKO; 1∶200); rat anti-Mac2 (Cedarlane;1∶2,000); mouse anti-NeuN (Millipore; 1∶1,000); rabbit anti-p53 (Leica; 1∶1,000). For avidin– biotin–peroxidase immunocytochemistry biotinylated secondary antibodies from Vector Laboratories, diluted 1∶200 were used. FITC-, Cy3-, and Cy5-conjugated secondary antibodies raised in donkey (Jackson ImmunoResearch) diluted at 1∶200 were used for confocal immunofluorescence.

Barnhoorn S., Uittenboogaard L.M., Jaarsma D., Vermeij W.P., Tresini M., Weymaere M., Menoni H., Brandt R.M., de Waard M.C., Botter S.M., Sarker A.H., Jaspers N.G., van der Horst G.T., Cooper P.K., Hoeijmakers J.H, & van der Pluijm I. (2014). Cell-Autonomous Progeroid Changes in Conditional Mouse Models for Repair Endonuclease XPG Deficiency. PLoS Genetics, 10(10), e1004686.

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Immunostaining for Retinal Cell Markers

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Tissue was fixed as described above. Immunostaining was performed as described in refs. 12 and 31. Antibodies used were as follows: chick and rabbit anti-GFP (1:500, Abcam; 1:2000, Millipore); mouse anti-Satb2(1:1000, Abcam); goat anti anti-CHX10 (1:300, Santa Cruz); rabbit anti-TFAP2B (1:1000, cell signaling); goat anti-Choline Acetyltransferase (1:500, Millipore); goat anti-VAChT (1:1000, Millipore); rabbit and guinea pig anti-RBPMS (1:1000, Abcam; 1:5000, PhosphoSolutions); rabbit anti-Calbindin (1:2000, Swant); mouse anti-Calretinin (1:5000, Millipore); mouse anti-Gad65/67 (1:1000, Millipore); mouse and rabbit anti-PKCa (1:2000, Abcam; 1:2000, sigma); rabbit anti-Secretagogin (1:10,000; BioVendor); mouse anti-Human CD15 (1:100, BD); rabbit anti-GNGT1 (1:1000, gift from Dr. N. Gautam), mouse anti-7G6 (1:1000, gift from Dr. Peter MacLeish), and chick anti-OPN1M/LW (1:2000, gift from Dr. Jeremy Nathans). Nuclei were labeled with DAPI (1:1000, Invitrogen). Secondary antibodies were conjugated to Alexa Fluor 488, 568, and 647 (Invitrogen) and used at 1:1000. Sections and whole mounts were mounted in ProLong Gold Antifade (Invitrogen).

Peng Y.R., Shekhar K., Yan W., Herrmann D., Sappington A., Bryman G.S., van Zyl T., Do M.T., Regev A, & Sanes J.R. (2019). MOLECULAR CLASSIFICATION AND COMPARATIVE TAXONOMICS OF FOVEAL AND PERIPHERAL CELLS IN PRIMATE RETINA. Cell, 176(5), 1222-1237.e22.

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Immunohistochemical Analysis of Retinal Cell Types

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The following antibodies were used in this study: mouse anti-POU4F1/BRN3A (Millipore, Darmstadt, Germany; MAB1585; 1:100); goat anti-POU4F2/BRN3B (Santa Cruz Biotechnology, Santa Cruz, CA; sc-6026; 1:100); rabbit anti-melanopsin (generous gift from Ignacio Provencio; 1:10,000); rabbit anti-tyrosine hydroxylase (Millipore; AB152; 1:500); mouse anti-AP2α (Developmental Studies Hybridoma Bank, Trevor J. Williams; 3B5; 1:50); rabbit anti-calbindin (Swant, Marly, Switzerland; CB-38a; 1:1,000); goat anti-choline acetyltansferase (Millipore; AB144P; 1:250); rabbit anti-bNOS (Sigma, St. Louis, MO; N7280; 1:5,000); mouse anti-PKCα (Santa Cruz Biotechnology, sc-17769; 1:500); rabbit anti-cone arrestin (Millipore; AB15282; 1:10,000); mouse anti-glutamine synthetase (Millipore; MAB302; 1:1,000); mouse anti-calsenilin (Millipore; 05–756; 1:1,000); mouse anti-PAX6 (developed by Kawakami, Developmental Studies Hybridoma; 1:500); and rabbit anti-Cre (Millipore; 69,050-3; 1:500). All secondary antibodies were used at 1:1,000 (Jackson ImmunoResearch, West Grove, PA).

Simmons A.B., Bloomsburg S.J., Billingslea S.A., Merrill M.M., Li S., Thomas M.W, & Fuerst P.G. (2016). Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers. Molecular Vision, 22, 705-717.

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