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7 protocols using arium comfort 2

1

Analytical-Grade Chemicals Protocol

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All chemicals and reagents used in this study were American Chemical Society (ACS) analytical grade unless stated otherwise. American Society for Testing and Materials (ASTM) type 1 deionized water was used for all experiments which was supplied from Ultrapure Water Purification Systems (Arium comfort 2, Sartorius, Germany). All the agar media (LB Agar, Nutrient Agar, Kligler's Iron agar, MIU medium base, and Simmon's citrate agar) used in the present study were procured from HiMedia Laboratories, India. 1, 5-diphenylcarbazide, Potassium dichromate (K2Cr2O7), Sodium chloride (0.85% NaCl), Sodium hydroxide (2 mol/L NaOH), Sulfuric Acid (0.2 mol/L H2SO4), Acetone, and Hydrogen peroxide (H2O2) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 1 mol/L HNO3 and NaOH solutions were prepared using analytical grade nitric acid (HNO3) and Sodium hydroxide (NaOH) and, were used to adjust the pH of the solution [38 ]. All stock solutions were kept in amber glass bottles in a dark place to prevent degradation.
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2

Synthesis of Semiconductor Nanocrystals

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Zinc stearate (Zn(St)2, purum, 10–12% Zn-based), zinc oxide (ZnO, ≥99.0%), caprylic acid (CA, ≥99.0%), 1-dodecanethiol (98%), tributylphosphine (TBP, 97%), selenium (Se, ≥99.5%), sulfur (S, purum), acetone (≥99.0%), n-hexane (≥95.0%), 1-octadecene (ODE, 90%), toluene (≥99.0%), MPA (≥99%), and MUA (95%) were acquired from Sigma Aldrich (St. Louis, MO, USA). Indium acetate (In(Ac)3, 99.99%) and trioctylphosphine (TOP, 90%) were acquired from Alfa Aesar. Tris(trimethylsilyl)phosphine (P(TMSi)3, 97.5%) was obtained from Vezerf Laborsynthesen GmbH (Haverhill, MA, USA). Glacial acetic acid (HOAc, ≥99.7%) was acquired from Fisher Scientific (Hampton, VA, USA). Sodium hydroxide solution (NaOH, 1 M) was purchased from Th. Geyer GmbH & Co. KG (Renningen, Germany). Ultrapure water type II (conductivity at 25 °C is 0.055 µS/cm) was obtained from a Sartorius Arium Comfort 2 (Göttingen, Germany). All the chemicals were used without further purification unless stated otherwise.
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3

Trace Metal Analysis in Chickpeas

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Chemicals and reagents used in this study were American Chemical Society (ACS) analytical grade unless stated otherwise. American Society for Testing and Materials (ASTM) type 1 deionized water (resistivity of 18.2 MΩ-cm) was used for all experiments supplied from Ultrapure Water Purification Systems (Arium comfort 2, Sartorius, Germany). Next, 1,5-diphenyl carbazide, NADH, K2Cr2O7, NiCl2.6H2O, Pb(NO3)2, Co(NO3).6H2O, ZnCl2, CdCl2, and CuSO4.5H2O were obtained from Sigma-Aldrich (St. Louis, MO, USA). MnCl2 was purchased from Fisher Scientific (Waltham, MA, USA). All stock solutions were stored in amber glass bottles in a dark environment. Chickpea seeds were obtained commercially from the retail market in the Dhaka city, Bangladesh.
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4

Analytical Standards for Chemical Analysis

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α-Terpineol (≥96% purity, natural); Polyoxyethylene(20) sorbitan monolaurate (Tween®20; HLB: 16.7) and alkane standard solution C8–C20 were acquired from Sigma-Aldrich (Tokyo, Japan). Ammonium sulfate ((NH4)2SO4); methanol (CH3OH); sodium azide (NaN3); sodium chloride (NaCl); sodium phosphate, monobasic (NaH2PO4 2H2O); sodium phosphate, dibasic (Na2HPO4 12H2O) and soybean oil were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). All products here mentioned were reagent grade and used without any further purification. RephiQuick non-sterile syringe filter (PTFE 32 mm; 0.45 μm) was acquired from RephiLe Bioscience (Shangai, China). Ultrapure grade water (ASTM Type 1/0.055 μS cm−1/>18.2 MΩ.cm at 25˚C) was processed by Arium® Comfort II (Sartorius AG, Göttingen) system.
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5

Development of D-Limonene-Based Emulsions

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D-limonene (LM), soybean oil (SB), caprylic acid (CP) and polyoxyethylene (80) sorbitan monooleate (Tween 80), sodium hydroxide, hydrochloric acid, and sodium azide were purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan). The current study prepared all the solutions and emulsions with water purified by an Ultrapure Water System (Arium® Comfort II, Sartorius Co., Göttingen, Germany). In addition, all the chemicals used in this study were analytically graded and applied accordingly throughout experimentation.
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6

Characterization of Exotic Heartwood Species

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Wood specimens of P. santalinus and P. tinctorius heartwood were supplied from Wood Collections of Chinese Academy of Forestry (WOODPEDIA). Indium-tin oxide (ITO) coated glass slides and double-sided conductive tape were purchased from China’s Sinopharm. ACN in chromatographic grade were purchased from Merck (Darmstadt, Germany). TFA were purchased from Sigma-Aldrich (Taufkirchen, Germany). ddH2O was purified by filtration system (Sartorius arium-comfort II, Germany). 2-MBT were supplied by Bruker Beijing laboratory (Bruker, Germany).
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7

Multi-compound Analysis by GC-MS

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Chromatographic analyses were carried out using Gas Chromatograph with Mass Spectrometry detector (GC-MS) by Agilent Technologies (Santa Clara, CA, USA) equipped with capillary columns by Sigma-Aldrich (Poznań, Poland).
Disposable SupercleanTM extraction tubes by Merck KGaA (Darmstadt, Germany) and organic solvents methanol (MeOH), acetonitrile (ACN) and dichloromethane (DCM) with a purity over 99% from Avantor Performance Materials Poland S.A. (Gliwice, Poland) were used for Solid Phase Extraction (SPE).
The deionized water was obtained from a laboratory water distillation station Arium Comfort II UV by Sartorius AG (Göttingen, Germany).
The analytical reference standards of food additives used during targeted chromatographic analysis (TCA) were delivered by Merck KGaA (Darmstadt, Germany).
The obtained mass spectra were compared with the United States National Institute of Standards and Technology NIST v17 Mass Spectral Library using MassHunter software.
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