CSCs seeded in 10 cm-diameter dishes were treated with H2O2 at the indicated concentrations for 5 h, then collected and stained with Annexin V-FITC/PI apoptosis kit (BioLegend, California, USA) according to the manufacturer's instructions, and detected by flow cytometer (Image StreamX MarkII, Amnis, USA). The results were analyzed by IDEAS software (Amnis, USA).
Annexin 5 fitc pi apoptosis kit
Manufactured by BioLegend
Sourced in United States
The Annexin V-FITC/PI apoptosis kit is a laboratory tool used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a DNA-binding dye, to distinguish between viable, early apoptotic, and late apoptotic/necrotic cells.
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Lab products found in correlation
4 protocols using annexin 5 fitc pi apoptosis kit
1
Oxidative Stress-Induced Apoptosis in CSCs
2
Cell Cycle and Apoptosis Analysis
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Whole-cell suspensions were stained with 50 μg/ml PI (propidium iodide) after 70% ethanol fixation and analyzed for cell cycle phase distribution with a BD Biosciences flow cytometer. Cell apoptosis was also quantified using an Annexin V-FITC/PI apoptosis kit (BioLegend, 640,914) according to the manufacturer’s instructions. The number of FITC- or PI-positive cells was determined using a flow cytometer (ImageStreamX MarkII, Amnis, USA). The fluorescence levels of FITC and PI were measured in the FL2 channel (488 nm) and FL4 channel (488 nm), respectively. The results were analyzed using IDEAS Application v6.0 software (Amnis, USA).
3
Annexin V FITC/PI Apoptosis Assay
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The processed HL-1 cells were harvested and adjusted to 1×106/mL. Then, the cells were processed using Annexin V FITC/PI apoptosis kit (BioLegend, San Diego, CA, USA). Flow cytometry was adopted to confirm the apoptosis rate.
4
Apoptosis Induction in sCAFs/rCAFs
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sCAFs or rCAFs were treated with either 8 μM cisplatin, 15 μM 5’FU or 0.1% DMSO (as a control) in the presence or absence of placebo, 10 μM AKT (Perifosine) or 20 μM p65 (Maslinic acid) inhibitors for 48 h. After the harvest, cells were subsequently treated with Annexin V-FITC/PI Apoptosis Kit (Cat no. #6409914, Biolegend) and finally analyzed by using a FCM (BD Accuri™ C6). The FACS gating strategies were given in Supplementary Fig. 8b .
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