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Zo 1 antibody pa5 28858

Manufactured by Thermo Fisher Scientific
Sourced in United States

The ZO-1 antibody (PA5-28858) is a primary antibody product developed by Thermo Fisher Scientific for research use. It is designed to detect the zonula occludens-1 (ZO-1) protein, which is a tight junction-associated protein involved in the regulation of cell-cell adhesion and the maintenance of epithelial and endothelial barrier function. The ZO-1 antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of the ZO-1 protein in different cell types and tissue samples.

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2 protocols using zo 1 antibody pa5 28858

1

Bile Acid Metabolism and Liver Function Assay

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Aspartate aminotransferase and alanine aminotransferase assay kits were purchased from Pointe Scientific (Canton. MI). GSK2330672 was purchased from MedChemExpress LLC (Monmouth Junction, NJ). Bile acid assay kit was purchased from Diazyme Laboratories (Poway, CA). AAV-FGF15 (under the control of an albumin promoter) was purchased from GeneCopoeia, Inc. (Rockville, MD). AAV-Null was purchased from Vector Biolabs Inc. (Malvern, PA). F4/80 antibody (Cat #. 70,076) was purchased from Cell Signaling Technology (Danvers, MA). Cytokeratin-19 antibody (ab52625) was purchased from Abcam (Waltham, MA). ZO-1 antibody (PA5-28858) was purchased from Thermo Fisher Scientific (Grand Island, NY).CDCA-d4 MaxSpec® Standard (CDCA-d4, No. 31366), tauro-conjugated CDCA-d4 MaxSpec® Standard (T-CDCA-d4, No. 31362), UDCA-d4 MaxSpec® Standard (UDCA-d4, No. 31368), and Tauro-conjugated UDCA-d4 MaxSpec® Standard (T-UDCA-d4, No. 31564) were purchased from Caymen Chemical Company (Ann Arbor, MI). FITC-dextran (MW:3000–5000) was purchased from Sigma Aldrich (St. Louis, MO).
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2

Liver Function Markers and Histology

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Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) assay kits were purchased from Pointe Scientific (Canton, MI, USA). A bile acid assay kit was purchased from Diazyme Laboratories (Poway, CA, USA). Glycine-conjugated β muricholic acid (G-β-MCA) was purchased from MedChemExpress Inc. (Monmouth Junction, NJ, USA). F4/80 antibody (Cat #. 70076) was purchased from Cell Signaling Technology (Danvers, MA, USA). CK19 antibody (ab52625) was purchased from Abcam (Waltham, MA, USA). ZO-1 antibody (PA5-28858) was purchased from ThermoFisher Scientific (Grand Island, NY, USA). Fluorescein isothiocyanate–dextran (FITC–dextran, MW:3000–5000) was purchased from Sigma Aldrich (St. Louis, MO, USA).
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