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Human cxcl12 sdf 1 elisa kit

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The Human CXCL12/SDF-1 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of human CXCL12 (C-X-C motif chemokine ligand 12), also known as stromal cell-derived factor 1 (SDF-1), in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Human c5a elisa kit, by BD (2 mentions) Human c5b 9 elisa kit, by BD (2 mentions) Human c3a elisa kit, by BD (1 mentions)

Close spelling variants of this product

Human CXCL12/SDF-1 DuoSet ELISA kit Human CXCL12/SDF-1 alpha Quantikine ELISA Kit Human CXCL12/SDF-1 kit CXCL12/SDF-1 Human CXCL12 ELISA kit CXCL12 ELISA kits Human CXCL12 SDF-1 CXCL12 Human kits

4 protocols using human cxcl12 sdf 1 elisa kit

1

Quantifying Complement and Chemokine Levels

Cited 2 times
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C5b-9 (MAC), C3a and C5a complement components were determined by using the Human C5b-9 ELISA Kit, Human C3a ELISA Kit and Human C5a ELISA Kit (BD OptEIA), respectively.
SDF-1 was determined using a Human CXCL12/SDF-1 ELISA Kit (R&D Systems) [58 (link), 62 (link)–64 (link)].
Jabłoński M., Mazur J.K., Tarnowski M., Dołęgowska B., Pędziwiatr D., Kubiś E., Budkowska M., Sałata D., Wysiecka J.P., Kazimierczak A., Reginia A., Ratajczak M.Z, & Samochowiec J. (2016). Mobilization of Peripheral Blood Stem Cells and Changes in the Concentration of Plasma Factors Influencing their Movement in Patients with Panic Disorder. Stem Cell Reviews, 13(2), 217-225.
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2

Quantifying SDF-1 in Diabetes

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Plasma from type 1 diabetes and control group subjects was used to evaluate concentration of SDF-1/CXCL12 (stromal cell-derived factor 1) concentration – factor participating in regulation of stem cells migration. SDF-1 levels were established in accordance with protocol included in the Human CXCL12/SDF-1 ELISA kit (R&D Systems). Data were acquired with the use of LEDETECT96 microplate reader (Labexim) set to 450 nm and wavelength correction included.
Jamiołkowska-Sztabkowska M., Grubczak K., Starosz A., Krętowska-Grunwald A., Krętowska M., Parfienowicz Z., Moniuszko M., Bossowski A, & Głowińska-Olszewska B. (2021). Circulating Hematopoietic (HSC) and Very-Small Embryonic like (VSEL) Stem Cells in Newly Diagnosed Childhood Diabetes type 1 – Novel Parameters of Beta Cell Destruction/Regeneration Balance and Possible Prognostic Factors of Future Disease Course. Stem Cell Reviews and Reports, 18(5), 1657-1667.
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3

Quantifying Complement and Chemokine Factors

Cited 2 times
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The Human C5b-9 ELISA Kit, Human C3ac ELISA Kit, and Human C5a ELISA Kit (BD OptEIA) were used to determine C5b-9 (membrane attack complex), C3a, and C5a complement components, respectively. The Human CXCL12/SDF-1 ELISA Kit (R&D Systems) was used to determine SDF-1 [40 (link),43 (link),46 (link),47 (link)].
Waszczuk K., Kucharska-Mazur J., Tyburski E., Rek-Owodziń K., Plichta P., Rudkowski K., Podwalski P., Grąźlewski T., Mak M., Misiak B., Michalczyk A., Tarnowski M., Sielatycka K., Szczęśniak A., Łuczkowska K., Dołęgowska B., Budkowska M., Ratajczak M.Z, & Samochowiec J. (2022). Psychopathology and Stem Cell Mobilization in Ultra-High Risk of Psychosis and First-Episode Psychosis Patients. International Journal of Environmental Research and Public Health, 19(10), 6001.
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4

CXCL12 Secretion Quantification in AT101-Treated Cells

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T265-2c cells were plated in 100mm dishes at previously described densities and were treated 24h post plating in media containing 2% FBS with or without AT101. Conditioned media was collected 24 hours after treatment and concentrated ~80-fold using Amicon Ultra-4 centrifugal filters with a molecular size exclusion of 3kD (Millipore, Billerica, MA UFC800324) and stored at -80°C. Following protein quantification, 375μg total protein was loaded per well (in duplicate) using a human CXCL12/SDF-1 ELISA kit (R&D Systems, Minneapolis, MN DSA00) per the manufacturer's instructions.
Graham C.D., Kaza N., Pruitt H.C., Gibson L.M., Klocke B.J., Shevde L.A., Carroll S.L, & Roth K.A. (2016). BH3 mimetics suppress CXCL12 expression in human malignant peripheral nerve sheath tumor cells. Oncotarget, 8(5), 8670-8678.
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