Zq 2000 esi mass spectrometry

uHPLC–MS analysis was achieved using a Waters Acquity uHPLC provided of a ZQ 2000 ESI mass spectrometry (Waters, Milford, MA, USA) and Mass Link software (Waters, Milford, MA, USA). The 2.6 µm Kinetex 50 mm × 4.6 mm C18 column was selected to perform the analysis. The mobile phases used were water with 0.1% formic acid as solvent A and acetonitrile with 0.1% formic acid as solvent B. The liquid chromatography ran in a gradient condition from 100% H₂O at t₀ to 100% acetonitrile at t₅ (5 min) under a flow rate of 0.3 mL/min. The column operated at a stationary temperature of 40 °C. Temperature, nebulizer pressure, and flow rate of drying gas (N₂) were 230 °C, 35 psi, and 10 L/min, respectively. The further operation parameters were 1200V for nozzle voltage and 2500 V for the capillary voltage. Mass spectra were tracked in a mass-to-charge (m/z) ratio range of 150–500 in positive ion detection mode.

uHPLC–MS analysis was conducted using a Waters Acquity uHPLC with ZQ 2000 ESI mass spectrometry (Waters, Milford, MA, USA) and Mass Link software (Waters, Milford, MA, USA). The 2.6 µm Kinetex 50 mm × 4.6 mm C18 column was selected to perform the analysis. The mobile phases used were water with 0.1% formic acid as solvent A and acetonitrile with 0.1% formic acid as solvent B. The liquid chromatography ran in a gradient condition from 100% H₂O at t0 to 100% acetonitrile at t5 (5 min) under a flow rate of 0.3 mL/min. The column operated at a stationary temperature of 40 °C. The temperature, nebulizer pressure, and flow rate of the drying gas (N2) were 230 °C, 35 psi, and 10 L/min, respectively. The further operation parameters were 1200 V for the nozzle voltage and 2500 V for the capillary voltage. Mass spectra were tracked in a mass–to–charge (m/z) ratio range of 150–500 in the positive ion-detection mode.