Following formalin fixation, tissues were embedded in paraffin. Sections (4um) were then prepared and mounted on slides for staining. Antigen retrival was performed using citrate buffer (pH 6.0) and steamed at high power for 17 min. Immunohistochemical staining of anti-NTN-1 (rabbit monoclonal [EPR5428] ab126729 Abcam Cambridge, UK) was achieved using the Ventana autostainer Discover XT (Ventana Medical Systems Oro Valley, AZ). Ultra-map DAB anti-Rb detection kit (760-151 Roche Basel, CH) was used as secondary antibody. Stained slides were digitalized with the SL801 autoloader and Leica SCN400 scanning system (Leica Microsystems Wetzlar, DE).
Ventana autostainer discover xt
Manufactured by Roche
Sourced in United Kingdom
The Ventana autostainer Discover XT is a fully automated slide staining system designed for advanced immunohistochemistry (IHC) and in situ hybridization (ISH) staining procedures. The system automates the entire staining process, including deparaffinization, rehydration, antigen retrieval, primary antibody and probe incubation, and detection. It is capable of processing multiple slide racks simultaneously, providing efficient and consistent results.
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Lab products found in correlation
2 protocols using ventana autostainer discover xt
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Immunohistochemical Analysis of NTN-1 Expression
2
Immunohistochemical Staining of Tumor Sections
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Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded 4-μm tumor sections using the appropriate primary antibody (mouse anti-CD31 and anti-pimonidazole antibodies) and the Ventana autostainer Discover XT (Ventana Medical Systems) using an enzyme-labeled biotin streptavidin system and solvent-resistant 3,3’-diaminobennyidine map kit. All staining intensity comparisons were made at 200 × magnification and scored independently by two histopathologists (Dr. L.F. and Dr. K.H.).
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