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Nitric acid

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Nitric acid is a clear, colorless to yellowish liquid chemical compound. It has the chemical formula HNO3. Nitric acid is a strong acid that is commonly used in various industrial and laboratory applications.

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9 protocols using nitric acid

1

Doxorubicin-Loaded Chitosan Nanoparticles

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Tetraethyl orthosilicate, chitosan (Mw, 200 kDa; deacetylation degree, 85%), sodium tripolyphosphate, doxorubicin hydrochloride (DOX) and dimethyl sulfoxide were obtained from Aladdin Chemistry Co., Ltd. (Shanghai, China). Chitosan oligosaccharide (COS, Mw, 3 kDa; deacetylation degree, 80%) was purchased from Golden-Shell Biochemical Co., Ltd. Diallyl disulfide, N,N′-methylenebisacrylamide, nitric acid and acetic acid were purchased from Macklin Biochemical Co., Ltd. Ceric ammonium nitrate, acrylic acid and methyl methacrylate were purchased from Sinopharm Chemical Reagent Co., Ltd. Glutathione was purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd.
Human cervical carcinoma cells (HeLa cells) and female BALB/c nude mice (5–6 weeks old, 18 ± 2 g) were supplied by Institute of Virology, Chinese Academy of Sciences. HeLa cells were cultivated in Dulbecco’s Modified Eagle medium containing 10% fetal bovine serum and 1% antibiotics. All cell line and animal experiments were executed in compliance with the Animal Management Rules of the Ministry of Health of the People’s Republic of China and approved by the Animal Care and Ethics Committee and Institutional Review Board of Wuhan Institute of Virology, Chine Academy of Sciences (Approval No. WIVA20201802).
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2

Polystyrene Sulfonate Scale Inhibitor Protocol

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Polystyrene sulfonate (PSS) with 30% activity (wt./wt.) and a molecular mass of 35,000 Da were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China) and used as a scale inhibitor. The molecular structure of PSS is shown as Figure S1 in Section S1 of the SI. Sodium dodecyl benzenesulfonate (SDBS) (>95%) was purchased from Meryer Chemical Technology Co., Ltd. (Shanghai, China). Chemicals such as barium chloride, calcium chloride, sodium chloride, nitric acid, hydrochloric acid, sodium hydroxide, sodium sulfate, and potassium bromide were in analytical grade and purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Calcite solid with a purity of 98.5% was purchased from Yanxi Mining Co., Ltd. (Shijiazhuang, China). Deionized water (DI water) was obtained using a Millipore Direct-Q 3UV system (Merck, Billerica, MA, USA).
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3

Preparation of Analytical Reagents

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Ethanol, acetic acid, and nitric acid were of analytical–reagent grade from Macklin Biochemical Co., Ltd. (Shanghai, China). Water was prepared by a ultrapure water system (Milli-Q, Millipore, MA, USA).
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4

Synthesis of Graphite-based Composites

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Graphite powers (carbon content 99 wt%) were purchased from Nanjing XFNANO Materials Tech Co.,Ltd (Nanjing, China). Chitosan (low viscosity: <200 mPa s), acetic acid, concentrated sulfuric acid (H2SO4, 98%), sodium nitrate (NaNO3, AR), hydrochloric acid (HCl, AR, 36 to 38%), potassium permanganate (KMnO4, AR), potassium ferrate (KFeO4), hydrogen peroxide (H2O2, 30%), potassium hydroxide (KOH, GR, 95%) and nitric acid (HNO3) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China).
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5

Synthesis and Characterization of Boron-containing Bioactive Glass

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First, the B-BG was synthesized according to our previously developed methods (Ai et al., 2020 (link)). Then, ethanol was briefly dissolved in deionized water under constant magnetic stirring followed by the addition of the respective amounts of the individual precursors. Notably, the precursors were added as stated in the order below and each precursor was ensured that it completely dissolved before the next one was added. Tetraethyl orthosilicate (TEO, Aladdin, Shanghai, China), calcium nitrate tetrahydrate (Aladdin, Shanghai, China), triethyl phosphate (TEP, Aladdin, Shanghai, China), and Trinbutyl Borate (TBB, Aladdin, Tianjin, China) were used as precursors for SiO2, CaO, P2O5, and B2O3, respectively. Subsequently, nitric acid (Macklin, Shanghai, China) was added to catalyze the hydrolysis reaction and the resulting solution stirred for 24 h at room temperature. Next, this solution was aged and dried for 48 h at a temperature of 80°C. The resulting gel was dried and calcinated for 2 h at a temperature of 800°C and a heating rate of 1°C min−1 and then naturally cooled in the furnace. Table 1 illustrates the different compositions that were used in the preparation of Boron-containing Bioactive glass samples such as 0B, 1B, and 2B. The calcined product was ground in a mortar and pestle to obtain a fine powder which was sieved through a 200 mesh (75 μm).
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6

Synthesis of Tungstate Nanostructures

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Sodium tungstate dihydrate (Na2WO4·2H2O, 99.5%), nitric acid (HNO3, 65–68%), and tetramethylammonium hydroxide (TMAOH, 96%) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China), Damao Chemical Reagent Factory (Tianjin, China), and Dubai Biological Technology Co., Ltd. (Shanghai, China), respectively. The above three chemical reagents were used as received without further purification.
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7

Triboelectric Charging of Liquid Droplets

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Redistilled solvents and Milli-Q water (>18
MΩ cm) were used for substrate cleaning and preparation of solutions.
An FEP (30 μm, DAIKIN) film was used for triboelectrification
with liquid droplets. Sodium chloride (99.5%), sodium iodide (99.5%),
sodium nitrate (99%), sodium bicarbonate (99.5%), sodium carbonate
(99.5%), sodium sulfite (98%), sodium sulfate (99%), magnesium sulfate
(99%), potassium chloride (99.5%), potassium ferricyanide (99.5%),
calcium chloride (97%), chromic nitrate (99%), manganese(II) chloride
(99%), manganese(II) sulfate (99%), ferric nitrate (98.5%), nickel(II)
chloride (99%), copper sulfate (99%), copper(II) nitrate (99%), zinc
nitrate (99%), zinc acetate (98%), hydrochloric acid (37%), sulfuric
acid (98%), nitric acid (68%), potassium hydroxide (99.9%), and sodium
hydroxide (97%) were purchased from Macklin. Ethanol (99.7%) and acetone
(99.5%) were obtained from Yong Da Chemical.
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8

Chemical Mechanical Polishing Solution Formulation

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The solutions in this work were prepared with deionized water, silica abrasives (40% purity; JinWei Group Co. Ltd., China) and analytically pure grade chemicals, in which silica abrasives were only used for CMP experiments and Zeta potential measurements. Chemicals including hydrogen peroxide (H2O2; 30% purity), ethylenediamine (C2H8N2; 99.5% purity), ethylenediamine tetraacetic acid (C10H16N2O8; 98% purity), glycine (C2H5NO2; 99% purity), potassium citrate (C6H5K3O7; 99.3% purity), 5-methyl-benzotriazole (C7H7N3, 99.9% purity), sodium dodecyl benzene sulfonate (C12H25SO3Na, 99.97% purity) and nitric acid (as pH adjuster) were obtained from Shanghai Macklin Biochemical Technology Co. Ltd while H2O2 purchased from Jiangtian Chemical Co. Ltd., Tianjin, China.
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9

Synthesis of Redox-Active Materials

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Ferric nitrate nonahydrate (Fe(NO3)3·9H2O), glycerol, isopropyl alcohol, absolute ethanol, polyethylene glycol (PEG 20000), nitric acid (HNO3), sulfuric acid (H2SO4), potassium permanganate (KMnO4), hydrogen peroxide (H2O2), sulphur powder and graphite powder were bought from Macklin. Lithium iodide (LiI), lithium perchlorate (LiClO4) and tetrabutyl ammonium iodide, 4-tert-butyl pyridine, acetonitrile, were obtained from Aladdin. All the chemical-grade agents were commercially purchased without further purification.
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