Sox2-CreER; ROSA26-lox-stop-lox-EYFP mice were recreated from commercially available strains (Sox2-CreER: 017593; R26-lsl-EYFP: 006148) sold by the Jackson Laboratory (Bar Harbor, ME) [27 (link)]. To induce Cre-mediated activity, mice were administered 2 mg tamoxifen (TAM; Sigma, St. Louis, MO) suspended in corn oil by intraperitoneal injection daily for 4 consecutive days. For in utero lineage tracing, a single pulse of 2 mg TAM with 1 mg progesterone (Sigma) was given to pregnant females at E11.5. All animal care and use was approved and monitored by the University of Chicago Institutional Animal Care and Use Committee.
Sox2 creer
Manufactured by Jackson ImmunoResearch
Sox2-CreER is a Cre recombinase fusion protein that is expressed under the control of the Sox2 promoter. It is designed for conditional gene manipulation in cells expressing the Sox2 transcription factor.
Automatically generated - may contain errors
Lab products found in correlation
Lgr5 egfp ires creer, by Jackson ImmunoResearch (2 mentions)
Progesterone, by Merck Group (1 mentions)
R26 lsl eyfp, by Jackson ImmunoResearch (1 mentions)
Tamoxifen tam, by Merck Group (1 mentions)
Shhgfp cre, by Jackson ImmunoResearch (1 mentions)
Prrx1 cre, by Jackson ImmunoResearch (1 mentions)
Plp1 creer, by Jackson ImmunoResearch (1 mentions)
Notch1flox flox, by Jackson ImmunoResearch (1 mentions)
4 oh tamoxifen, by Merck Group (1 mentions)
Math1 gfp, by Jackson ImmunoResearch (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Cd 1 mice, by Charles River Laboratories (1 mentions)
Rosa26 lsl tdtomato, by Jackson ImmunoResearch (1 mentions)
5 protocols using sox2 creer
1
Inducible Cre-mediated Lineage Tracing
2
Conditional Genetic Manipulation in Mice
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All animal experiments were carried out according to protocols approved by the Institutional Animal Care and Use Committee at Harvard Medical School. Mouse lines are described in published literatures and purchased from the Jackson Laboratory: ShhGFP-Cre (Jackson Laboratory stock no. 005622), Prrx1Cre (Jackson Laboratory stock no. 005584), Sox2CreER (Jackson Laboratory stock no. 017593), tdTMTtg/+(Rosa26-TdTomato; Jackson Laboratory stock no. 007909), Tazf/f/Yapf/f (Jackson Laboratory stock no. 030532), conditional Yap* or Yapgof (Rosa26lox-stop-lox-rtTA/+;
Col1a1Teto-YapS127A/+) (38 (link)), Ptch1LacZ (53 (link)), and Ctgf-GFP (38 (link)). Timed mating of heterozygous intercrosses was performed to generate embryos of the indicated embryonic stage. The day in which a vaginal plug was confirmed was designated as E0.5. Mutant and transgenic embryos were processed in parallel with littermate controls. For the ShhCre;Yap* embryos, Dox was administered to pregnant female mice at 0.2 mg/ml in drinking water starting at E8.5. For Sox2CreER;Yap* embryos, Dox (same as above) and TM were administered at E7.5 (via intraperitoneal injection of the pregnant females (75 mg/kg of body weight).
Col1a1Teto-YapS127A/+) (38 (link)), Ptch1LacZ (53 (link)), and Ctgf-GFP (38 (link)). Timed mating of heterozygous intercrosses was performed to generate embryos of the indicated embryonic stage. The day in which a vaginal plug was confirmed was designated as E0.5. Mutant and transgenic embryos were processed in parallel with littermate controls. For the ShhCre;Yap* embryos, Dox was administered to pregnant female mice at 0.2 mg/ml in drinking water starting at E8.5. For Sox2CreER;Yap* embryos, Dox (same as above) and TM were administered at E7.5 (via intraperitoneal injection of the pregnant females (75 mg/kg of body weight).
3
Genetic Labeling of Diverse Cell Lineages
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Lgr5-EGFP-IRES-CreER (no. 008875) (Barker et al., 2007 (link)), Plp1-CreER (no. 005975) (Doerflinger et al., 2003 (link)), Sox2-CreER (no. 017593) (Arnold et al., 2011 (link)), and Rosa26-ACTB-mTmG (no. 007676) (Muzumdar et al., 2007 (link)) were obtained from the Jackson Laboratory. Rosa26-CAG-STOP-Cas9-tdTomato (Rosa26-tdTomato) mice were obtained from GemPharmatech, China. Pou4f3-EGFP-IRES-CreER or Pou4f3(EGFP/+) knockin mice were generated using the CRISPR-Cas9 genome-editing technology (Du et al., 2020 (link); Zhu et al., 2020 (link)). Both male and female mice on a C57BL6 background were used in this study. All animal procedures were approved by the Institutional Animal Care and Use Committee of Model Animal Research Center of Nanjing University, China, with protocol approval no. WGQ01.
4
Transgenic Mouse Models for Lineage Tracing
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Sox2-CreER, Math1-GFP, Lgr5-EGFP-IRES-CreER (referred as Lgr5-GFP), Stat3flox/flox, and Notch1flox/flox mice were purchased from Jackson laboratory. For Cre activation, 100 μL tamoxifen (10 mg/mL, Sigma) was administered to pregnant mice via gavage. 4-OH tamoxifen (100 mg/L, Sigma) was added into medium to induce Cre activity in vitro. All mouse experiments were approved by the Institutional Animal Care and Use Committee at the School of Biomedical Engineering, Shanghai Jiao Tong University.
5
Inducible Neurogenesis and Cancer Modeling
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Rosa26-LSL-MYC (Stock no. 020458), Rosa26-LSL-tdTomato (Stock no. 007908), Math1-creER (Stock no. 007684), Ascl1-creER (Stock no. 012882), and Sox2-creER (Stock no. 017593) were purchased from the Jackson laboratory. CD1 mice were purchased from Charles River Laboratories. Mice were intraperitoneally injected with TMX (75 mg/kg) at E9.5, E10.5, E13.5, P0, P1, P2, P5, P7, or P9. Animals were sacrificed at E15.5, P4, P7, P10, P14, P21, P30, P75, or at a humane end point as they displayed signs of morbidity (ataxia, weight loss, and ruffled fur). Mice were housed in a certified animal facility in accordance with European Guidelines. The experiments were approved by the Italian Ministry of Health as conforming to the relevant regulatory standards.
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