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Rabbit anti phospho irf3 ser396

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Rabbit anti-phospho-IRF3 (Ser396) is a primary antibody that recognizes IRF3 (Interferon Regulatory Factor 3) phosphorylated at serine 396. It is used to detect the activation of the IRF3 transcription factor.

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Lab products found in correlation

Rabbit anti phospho stat1 tyr701, by Cell Signaling Technology (2 mentions) Goat anti mouse il 1β, by R&D Systems (1 mentions) Rabbit anti mlkl phospho s345, by Abcam (1 mentions) Rabbit anti irf3, by Cell Signaling Technology (1 mentions) Rabbit anti caspase 3, by Cell Signaling Technology (1 mentions) Rabbit anti gapdh, by Merck Group (1 mentions) Mouse anti α tubulin, by Santa Cruz Biotechnology (1 mentions) Rabbit anti sting, by Cell Signaling Technology (1 mentions) Femtolucent plus hrp, by G Biosciences (1 mentions) Rabbit anti tbk1, by Cell Signaling Technology (1 mentions) Rabbit anti cytoskeletal actin, by Fortis Life Sciences (1 mentions) Mouse anti cvb3 vp1, by Agilent Technologies (1 mentions) Imagequant las 4000 mini system, by GE Healthcare (1 mentions) λ phosphatase, by New England Biolabs (1 mentions) Cd16 cd32, by Thermo Fisher Scientific (1 mentions) Igm apc, by Thermo Fisher Scientific (1 mentions) Mhc class 2, by Thermo Fisher Scientific (1 mentions) B220 percp, by Thermo Fisher Scientific (1 mentions) Rabbit anti phospho tbk1 ser172, by Cell Signaling Technology (1 mentions) Rat igg apc, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-IRF3 (65 citations) Phospho-IRF3 (41 citations) Anti-phospho-IRF3 (38 citations) Anti-phospho-IRF3 (Ser396) (12 citations) Phospho-IRF3(Ser396) (12 citations) Rabbit anti-IRF3 (10 citations) Rabbit anti-phospho-IRF3 (5 citations)

4 protocols using rabbit anti phospho irf3 ser396

1

Immunoblotting of Cell Death Regulators

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The primary antibodies used for immunoblotting were mouse IgG1 anti-caspase-1 (p20) (Adipogen, #AG-20B-0042, 1/2000 dilution), mouse IgG2b anti-NLRP3 (Adipogen, #AG-20B-0014, 1/1000), goat anti-mouse IL-1β (R&D systems, #AF-401-NA, 1/1000), rabbit anti-GAPDH (Sigma-Aldrich, #G9545, 1/20,000), rabbit anti-IRF3 (Cell Signaling, #4302, 1/2000), rabbit anti-phospho-IRF3 (Ser396) (Cell Signaling, #4947, 1/2000), rabbit anti-MAVS (rodent specific) (Cell Signaling, # 4983, 1/1000), rabbit anti-phospho-STAT1 (Tyr701) (Cell Signaling, #9171, 1/1000), rabbit anti-STAT1 (D1K9Y) (Cell Signaling, #14994, 1/3000), rabbit anti-caspase-3 (Cell Signaling, #9662, 1/1000), mouse IgG1 anti-DDX33 (B-4) (Santa Cruz, #sc-390573, 1/1000), rabbit anti-MLKL (phospho S345) (Abcam, #ab196436, 1/1000), rabbit anti-MLKL (D6W1K) (Cell Signaling, #37705, 1/2000), rabbit anti-phospho-DRP1 (Ser616) (D9A1) (Cell Signaling, #4494, 1/1000), mouse IgG1 anti-DRP1 (BD Biosciences, #611113, 1/2000), rabbit anti-RIPK1 (D94C12) (Cell Signaling, #3493, 1/2000), and guinea pig anti-mouse gasdermin D (Adipogen, #AG-25B-0036, 1/1000).
da Costa L.S., Outlioua A., Anginot A., Akarid K, & Arnoult D. (2019). RNA viruses promote activation of the NLRP3 inflammasome through cytopathogenic effect-induced potassium efflux. Cell Death & Disease, 10(5), 346.
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2

Immunoblotting Analysis of Cytoskeletal and Immune Signaling Proteins

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Rabbit anti-cytoskeletal actin (Bethyl Laboratories, Montgomery, TX, USA), mouse anti-CVB3 VP1 (Dako, Copenhagen, Denmark), mouse anti-α tubulin (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-STING, rabbit anti-phospho-STING (Ser336), rabbit anti-TBK-1, rabbit anti-phospho-TBK-1 (Ser172), rabbit anti-IRF-3, and rabbit anti-phospho-IRF-3 (Ser396) (all from Cell Signaling Technologies, Danvers, MA, USA) antibodies were used. The enhanced chemiluminescence substrate femto LUCENT™ PLUS-HRP (G-Biosciences, St. Louis, MO, USA) was applied, and images of bands were captured using an Image Quant™ LAS 4000 Mini system (GE Healthcare Life Sciences, Little Chalfont, UK). Quantification of band densities was performed using ImageJ software (NIH, Bethesda, MD, USA).
Song J.H., Ahn J.H., Kim S.R., Cho S., Hong E.H., Kwon B.E., Kim D.E., Choi M., Choi H.J., Cha Y., Chang S.Y, & Ko H.J. (2019). Manassantin B shows antiviral activity against coxsackievirus B3 infection by activation of the STING/TBK-1/IRF3 signalling pathway. Scientific Reports, 9, 9413.
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3

Multiparameter Flow Cytometry of RAE1 Expression

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The following antibodies were used: pan-RAE1, RAE1αβγ, RAE1βδ, RAE1ε (R&D Systems), B220-PerCP, IgM-APC, CD16/CD32, MHC class II (eBioscience), rabbit-anti-phospho-IRF3-Ser396 or rabbit-anti-phospho-TBK1-Ser172 (Cell Signaling Technology) and rat IgG-APC (eBioscience) or rabbit IgG-Alexa-488 (Invitrogen). 1 μg/ml propidium iodide (PI) was added to all stainings and PI negative cells are shown. For intracellular staining, cells were fixed according to the manufacturer's protocol. Some cells were treated with 2 U/μl λ-phosphatase (NEB) at 37°C for 90 min before staining. Stained cells were analyzed using FACSCalibur and FlowJo. 8.8.7. (Treestar). BrdU incorporation analysis were performed as described (19 ).
Lam A.R., Bert N.L., Ho S.S., Shen Y.J., Tang L.F., Xiong G.M., Croxford J.L., Koo C.X., Ishii K.J., Akira S., Raulet D.H, & Gasser S. (2014). RAE-1 ligands for the NKG2D receptor are regulated by STING-dependent DNA sensor pathways in lymphoma. Cancer research, 74(8), 2193-2203.
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4

Western Blotting and Immunostaining Antibodies

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The following antibodies were used for Western blotting and/or immunostaining: mouse anti-β-actin (BA3R; Invitrogen; RRID AB_10979409), rabbit anti-STAT1 (M-22; Santa Cruz; RRID AB_632434), rabbit anti-ISG15 (H-150; Santa Cruz; RRID AB_2126309), rabbit anti-IFIT1 (Invitrogen catalog no. PA5-27907 for human/nonhuman primate samples; RRID AB_2545383), rabbit anti-IFIT1 (Invitrogen catalog no. PA3-846 for murine samples; RRID AB_1958734), rabbit anti-phospho-STAT1 (Tyr-701) (catalog no. 9171; Cell Signaling Technology, Inc.; used for Western blotting assays; RRID AB_561284), rabbit anti-phospho-STAT1 (Tyr-701) (D4A7; Cell Signaling Technology, Inc.; used for immunofluorescence; RRID AB_10950970), rabbit anti-phospho-STAT1 (Ser-727) (catalog no. 9177; Cell Signaling Technology, Inc.; RRID AB_2197983), and rabbit anti-phospho-IRF3 (Ser-396) (catalog no. 29047; Cell Signaling Technology, Inc.). Antiflavivirus group antigen D1-4G2-4-15 (ATCC HB-112 hybridoma; RRID CVCL_J890) and mouse anti-YFV ascites fluid were used in flavivirus focus-forming assays. Human and murine IFNs α4 and β were prepared in-house as described previously (36 (link)). Lipopolysaccharides (LPSs) from Escherichia coli 0111:B4 were purchased from Sigma. Poly(I:C) was purchased from R&D Systems.
Lane W.C., Dunn M.D., Gardner C.L., Lam L.K., Watson A.M., Hartman A.L., Ryman K.D, & Klimstra W.B. (2018). The Efficacy of the Interferon Alpha/Beta Response versus Arboviruses Is Temperature Dependent. mBio, 9(2), e00535-18.
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