Normal human astrocytes (NHA) were maintained in astrocyte media (Welgene) supplemented with 10% fetal bovine serum (FBS; HyClone, Thermo Fisher Scientific), and 1% penicillin‐streptomycin (Welgene). Glioma cells (A172, A1207, and U87MG) were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 (Welgene) supplemented with 10% fetal bovine serum (FBS; HyClone, Thermo Fisher Scientific) and 1% penicillin‐streptomycin (Welgene). GBM patient‐derived glioma stem cells (GSC11, GSC20, GSC23, and GSC267) were provided by The University of Texas MD Anderson Cancer Center. GSCs were cultured under stem cell culture conditions (NBE, serum‐free neurobasal media supplemented with growth factors). The NBE comprised DMEM/F12 (Welgene) supplemented with B27 (Gibco), 1% penicillin/streptomycin (Welgene), epidermal growth factor (EGF; 20 ng/mL; R&D Systems), and basic fibroblast growth factor (bFGF; 20 ng/mL; R&D Systems).14 Growth factors (bFGF and EGF) were added twice a week. To induce differentiation, GSC11 and GSC23 cells were cultured for 10 days in DMEM/F12 containing 10% FBS.
Astrocyte media
Manufactured by Welgene
Sourced in Cameroon
Astrocyte media is a specialized culture medium designed for the growth and maintenance of astrocytes, which are a type of glial cell found in the central nervous system. This media provides the necessary nutrients and growth factors to support the in vitro cultivation of astrocytes, which are essential for various research and applications related to neuroscience and cellular biology.
Automatically generated - may contain errors
Lab products found in correlation
Hyclone, by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Welgene (2 mentions)
Penicillin streptomycin, by Welgene (1 mentions)
Basic fibroblast growth factor (bfgf), by R&D Systems (1 mentions)
Epidermal growth factor (egf), by R&D Systems (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
2 protocols using astrocyte media
1
Culturing Astrocytes and Glioma Cells
2
Culturing Normal Astrocytes and Glioma Cells
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Normal human astrocytes (NHA) were cultured in astrocyte media (Welgene, South Korea) supplemented with10 % fetal bovine serum (FBS; HyClone, Thermo Fisher Scientific, USA), Astrocyte Growth Supplement (AGS; Welgene, South Korea), and 1 % penicillin-streptomycin (P/S; Welgene, South Korea). Glioma cells (U87MG, A172, and A1207) were maintained in Dulbecco's modified Eagle's medium (DMEM/F-12; Welgene, South Korea) supplemented with 10 % FBS and 1 % P/S. The patient-derived glioma stem cells (GSC267, GSC11, GSC23, and GSC20) were sourced from The University of Texas MD Anderson Cancer Center. GSCs were cultured in serum-free neurobasal media (NBE) composed of DMEM/F-12, 2 % B-27 (Glico, Thermo-Fisher, USA), 1 % P/S, 20 ng/ul basic fibroblast growth factor (bFGF; R&D Systems), and 20 ng/ul basic epidermal growth factor (EGF; R&D Systems). For GSC differentiation, GSC11 and GSC23 cells were cultured for 10 days in DMEM/F-12 supplemented with 10 % FBS.
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