Total proteins from cells were lysed with RIPA buffer (Biouniquer Technology). Lysates were then separated by SDS-PAGE, and proteins were further transferred onto PVDF membranes (Sigma, Germany). After 5% non-fat dry milk blocking and overnight incubation with primary antibodies FOXP2, P-glycoprotein (P-gp), caspase-3, cleaved caspase-3, PARP, cleaved PARP, and GAPDH (1: 1000; Cell Signaling Technology). The blots were detected using appropriate secondary horseradish peroxidase-conjugated secondary antibodies (Sigma) and visualized by enhanced chemiluminescence detection (Millipore Corp., Billerica, MA, USA).
Foxp2
Manufactured by Cell Signaling Technology
FOXP2 is a transcription factor that regulates the expression of genes involved in various cellular processes. It plays a crucial role in the development and function of the nervous system, particularly in the areas of language and speech.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence detection, by Merck Group (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Cleaved parp, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Fibronectin, by Cell Signaling Technology (1 mentions)
Tgf β, by Cell Signaling Technology (1 mentions)
α catenin, by Cell Signaling Technology (1 mentions)
Snail, by Cell Signaling Technology (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
2 protocols using foxp2
1
Western Blot Analysis of Cellular Proteins
2
Western Blot Analysis of EMT Markers
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Western blot assay was performed as previously described 20 (link). Antibodies used were described as follow: FOXP2 (Cell signaling, #8198), Vimentin (Cell signaling, #5741), E-Cadherin (Cell signaling, #3195), α-Catenin (Cell signaling, #2163), Fibronectin (Cell signaling, #4706), Snail (Cell signaling, #3879), TGF-β (Cell signaling, #3709) and GFP (Cell signaling, #2956). The membranes were stripped and re-probed with anti-GAPDH (Sigma, G9545) or anti-β-actin (Sigma, SAB5500001) antibodies as loading control.
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