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9 protocols using nitric acid

1

Synthesis of Iron Nitrate Nanoparticles

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AHM, iron nitrate nonahydrate and methanol were purchased from Sinopharm Chemical Reagent Ltd. Corporation. Nitric acid and ammonia, with mass fraction of 65–68% and 25–28%, respectively, were obtained from Beijing Chemical Works. All reagents were analytical grade and used without further purification.
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2

Synthesis of Magnetic Nanoparticles

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Platinum acetylacetonate [Pt(acac)2, purity 97%], magnesium acetylacetonate [Mg(acac)2·2H2O, purity 98%], cobalt acetylacetonate [Co(acac)2, purity 97%], oleylamine (OAm, 70%) and oleic acid (OA, 90%) was purchased from Sigma Aldrich. Pentacarbonyl iron [Fe(CO)5 purity 99%], nitric acid(HNO3, concentration 68%), methylbenzene(purity 99%) and absolute ethyl alcohol was purchased from Beijing Chemical Works. All chemicals were used as received.
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3

Synthesis of Zirconium Oxynitrate and Acetaldehyde

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Zirconium oxynitrate (AR) and acetaldehyde (97%) were purchased from Aladdin Industrial Corporation. Neat ethanol (AR) was purchased from Tianjin Jiangtian Chemical Technology Co., Ltd. Nitric acid (AR) was purchased from Beijing Chemical Works. Tetraethyl orthosilicate (AR) and zinc nitrate hexahydrate (AR) were purchased from Tianjin Guangfu Chemical Reagent Co., Ltd.
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4

Membrane Dispersion Microreactor Synthesis

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Ammonium
phosphate ((NH4)3PO4·3H2O, ≥95%) and ferric nitrate (Fe(NO3)3·9H2O, ≥98.5%) were obtained from Sinopharm
Chemical Reagent Co., Ltd. Nitric acid (HNO3, 65–68%),
sulfuric acid (H2SO4, 95–98%), and phosphoric
acid (H3PO4, 85%) were obtained from Beijing
Chemical Works. All of the chemicals were used as received. Carbon
nanotubes (CNTs, FT9101) were obtained from Cnano Technology Co.,
Ltd.
The membrane dispersion microreactor device mainly comprised
two stainless steel sample plates (50 mm × 50 mm × 18 mm)
and a stainless steel microfiltration membrane. The membrane with
a pore size of 5 μm was used in the microreactor as the dispersion
medium. The active area of the microporous membrane was 12.56 mm2, and the geometric size of the microchannel was 15 mm ×
0.5 mm × 0.5 mm (length × width × height).
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5

Tyrosinase Inhibitor Screening and Evaluation

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Tyrosinase (TYR, EC1.14.18.1) from mushroom, l-3,4-dihydroxyphenylalanine (l-DOPA), kojic acid (KA), catechin, ECG, and EGCG were purchased from Sigma-Aldrich. Nitric acid and dimethyl formamide (DMF) were purchased from Beijing Chemical Works. Multiwalled carbon nanotubes (MWCNTs) were purchased from the Nanotech Port Co. Ltd (Shenzhen, China). Before use, the MWCNTs were acidified by Nitric acid.23 (link)Spectrophotometer reader (BioTek Instruments Inc., USA) and High Performance Liquid Chromatograph (HPLC: Shimadzu Instruments Inc., Japan) were used to measure the inhibitory activity of the Tyrosinase inhibitors. CV experiments were performed in a standard three-electrode electrochemical cell with a CHI 660E electrochemical analyzer (CH Instruments, Chenhua Co., Shanghai, China). Oxygen consumptions of Tyrosinase interacting with TPs were monitored with YSI 5000 dissolved oxygen meter (YSI Inc., American).
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6

Titanium Powder Synthesis and Purification

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All chemicals are analytical grade reagents
and are used as received without further purification. Potassium hydroxide
(KOH, ≥90%), sodium hydroxide (NaOH, ≥96%), sulfuric
acid (H2SO4, 98.3%), hydrochloric acid (HCl,
38%), and nitric acid (HNO3, 68%) are purchased from Beijing
Chemical Works. Ti powder (∼100 mesh) is purchased from Aladdin
Reagent Co., Ltd. Deionized water with a specific resistance of 18.2
MΩ cm is obtained by reverse osmosis, followed by ion exchange
and filtration.
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7

Biopolymer Synthesis Protocol

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Urea (99.9%) was purchased from Macklin (Shanghai, China). Chitosan powder (Mw = 300,000) was supplied from Zhejiang Golden Shell Biopharmaceutical (Yuhuan, China). Acetic acid (99.7%), nitric acid (69%), sulfuric acid (98%), hydrogen peroxide (30%) were analytical reagents and were purchased from Beijing Chemical Works (Beijing, China). N-hydroxysuccinimide (NHS), 1-(3-Dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride (EDC.HCl, 98%) were of analytical grade and were obtained from Aladdin Biochemical Technology (Beijing, China).
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8

Luminescent Gd2O3:Eu3+ Nanophosphors

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Gadolinium
oxide (Gd2O3, 99.9%), europium oxide (Eu2O3, 99.9%), boric
acid (H3BO3, >99.5%), and acetylacetone (C5H8O2, 99%) were bought from Aladdin
Industrial Co. Ltd. (Shanghai, China). Nitric acid (HNO3, 65%), urea ((NH2)2CO, 99%), and ethanol (C2H5OH, 98%) were purchased from Beijing Chemical
Works (Beijing, China), and poly(ethylene glycol)-1000 (PEG, 99%)
was obtained from Bioruler Co. Ltd. All of the materials were used
without further purification, unless otherwise stated. Deionized water,
used for all processes, was prepared by the Hitech laboratory water
purification system DW100 (Shanghai Hitech Instruments Co., Ltd.).
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9

Graphene Oxide Composite Synthesis

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Concentrated sulfuric acid (H 2 SO 4 , 98%), nitric acid (HNO 3 , 70%), sodium nitrate (NaNO 3 ), potassium permanganate (KMnO 4 ), hydrogen peroxide (H 2 O 2 , 38%), hydrochloric acid (HCl, 38%), ethanol, and ascorbic acid were purchased from Beijing Chemical Factory (China). Pristine graphite was supplied by Huadong Graphite Factory (China). Epoxy resin (JY-257) with an epoxy value of 0.53-0.55 mol/100g was obtained from Jiafa Chemical Co. (China). Methyl hexahydrophthalic anhydride as the curing agent and tris-(dimethyl aminomethyl) phenol as the curing accelerator were purchased from Alfa Aesar (China).
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