Analytical grade rosuvastatin calcium (>99%), sodium dihydrogen phosphate (≥99.9%), formic acid (>98%), leucine enkephalin (>98%), and isopropanol (>99%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). High purity nitrous oxide was purchased from Messer Croatia Plin (Zaprešić, Croatia) and acetonitrile (≥99.9%) from Merck Millipore (Burlington, MA, USA). Ultrapure water (18 MΩ·cm) was generated in-house using a Milli-Q System from Merck Millipore (Burlington, MA, USA).
Rosuvastatin calcium
Manufactured by Merck Group
Sourced in United States
Rosuvastatin calcium is a laboratory reagent used in the analysis and research of pharmaceutical compounds. It is a pharmaceutical active ingredient commonly used in the production of cholesterol-lowering medications. Rosuvastatin calcium serves as a reference standard for the identification and quantification of this compound in samples.
Automatically generated - may contain errors
Lab products found in correlation
Simvastatin, by Merck Group (3 mentions)
Fluvastatin sodium hydrate, by Merck Group (2 mentions)
Pravastatin sodium salt hydrate, by Merck Group (2 mentions)
Atorvastatin calcium salt trihydrate, by Merck Group (2 mentions)
Sodium dihydrogen phosphate, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Leucine enkephalin, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
C2c12 cell line, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
Lovastatin, by Merck Group (1 mentions)
Oligomycin a, by Merck Group (1 mentions)
Nutlin 3, by Selleck Chemicals (1 mentions)
Atorvastatin, by Cayman Chemical (1 mentions)
Sodium dichloroacetate, by Merck Group (1 mentions)
Pitavastatin, by Cayman Chemical (1 mentions)
Fluvastatin sodium, by Merck Group (1 mentions)
5 protocols using rosuvastatin calcium
1
High-Purity Compound Analysis
2
Commercially Obtained Statin Compounds
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The chemicals used in this research were commercially obtained: simvastatin (>97%, Sigma Aldrich Inc., USA), rosuvastatin calcium (>98%, Sigma Aldrich Inc., USA), fluvastatin sodium hydrate (>98%, Sigma Aldrich Inc., USA, Cas number 93957–55-2), atorvastatin calcium salt trihydrate (>98%, Sigma Aldrich Inc., Cas number 344423–98-9) and pravastatin sodium salt hydrate (>98%, Sigma Aldrich Inc., Cas number 81131–70-6).
3
Rhabdomyosarcoma and myoblast cell culture with statins
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The human rhabdomyosarcoma cell line A-204 and the mouse myoblast C2C12 cell line were obtained from ATCC, and maintained in McCoy's 5A medium (Gibco) and Dulbecco's modified Eagle's medium (Gibco) respectively. C2C12 myoblasts were stimulated to differentiate into myotubes by switching to the medium with 2% horse serum. The human colon cancer HCT116 SCO2−/− cell line was a generous gift from Dr. Paul M. Hwang at the National Institutes of Health, and cultured in McCoy's 5A medium.
Lovastatin, fluvastatin sodium, simvastatin, rosuvastatin calcium, mevalonic acid sodium, sodium dichloroacetate, coenzyme Q10 (CoQ10) and oligomycin A were purchased from Sigma Aldrich. Pitavastatin and atorvastatin were purchased from Cayman Chemical. Nutlin-3 was purchased from Selleck Chemicals. The doses of statins in cell culture were consistent with previous studies in SAMS [18 (link)].
Lovastatin, fluvastatin sodium, simvastatin, rosuvastatin calcium, mevalonic acid sodium, sodium dichloroacetate, coenzyme Q10 (CoQ10) and oligomycin A were purchased from Sigma Aldrich. Pitavastatin and atorvastatin were purchased from Cayman Chemical. Nutlin-3 was purchased from Selleck Chemicals. The doses of statins in cell culture were consistent with previous studies in SAMS [18 (link)].
4
Statin and Chloroquine Antiviral Assay
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Atorvastatin calcium salt trihydrate (ATO), cerivastatin sodium salt hydrate (CER), fluvastatin sodium hydrate (FLU), pravastatin sodium salt hydrate (PRA), rosuvastatin calcium (ROS), simvastatin (SIM), and chloroquine (CHL) were purchased from Sigma-Aldrich. Lovastatin sodium salt (LOV) and mevastatin sodium salt (MEV) were purchased from Calbiochem. The statins ATO, FLU, PRA, ROS, MEV, LOV, and SIM were reconstituted to 20 mM in dimethyl sulfoxide (DMSO); PRA was reconstituted to 50 mM in DMSO; CER was reconstituted to 20 mM in distilled deionized water. All statins were stored at −20 °C and diluted to working concentrations in infection medium on the same day of use. CHL was dissolved to 100 mM in distilled deionized water, stored at 4 °C, and diluted to the required concentration on the same day of use.
Mouse anti-flavivirus group antigen monoclonal antibody clone D1-4G2-4-15 (4G2 mAb) was purchased from Merck-Millipore (MAB10216) and stored at −20 °C in aliquots prior to use. The secondary antibody, fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG, was purchased from Sigma-Aldrich. The cytotoxicity assay kit, EZ-CYTOX, was purchased from DoGenBio, Co., Ltd. in South Korea.
Mouse anti-flavivirus group antigen monoclonal antibody clone D1-4G2-4-15 (4G2 mAb) was purchased from Merck-Millipore (MAB10216) and stored at −20 °C in aliquots prior to use. The secondary antibody, fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG, was purchased from Sigma-Aldrich. The cytotoxicity assay kit, EZ-CYTOX, was purchased from DoGenBio, Co., Ltd. in South Korea.
5
Rosuvastatin Quantification Protocol
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Rosuvastatin calcium, acetonitrile (LC-MS grade), water (LC-MS grade), formic acid (LC-MS grade), and methanol (LC-MS grade) were purchased from Sigma-Aldrich (Saint Louis, USA). Rosuvastatin-d3 Sodium salt (purity ≥ 95%) was supplied by Toronto Research Chemicals (Toronto, Canada). Stock solution was prepared by dissolving Rosuvastatin calcium in DMSO to achieve rosuvastatin concentration of 10 mg/mL. This solution was further diluted with water for injection to achieve dosing solution with concentration of 1 mg/mL. Isoflurane was used as IsoFlo 250 mL (Zoetis, Parsippany, USA).
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