Crude extracts, fractions, and pure compounds were evaluated for germination and growth inhibition of dicotyledon and monocotyledon Lactuca sativa L. (lettuce) and Agrostis stolonifera L. (bentgrass), respectively, in 24-well plates using a previously described method [31 (link)]. Plates were incubated in a Percival Scientific CU-36 L5 incubator under 16:8 h light/dark conditions at 26 °C and 120 μmol s−1 m−2 average photosynthetically active photon flux. After 7 days for L. sativa and 10 days for A. stolonifera, seed germination and growth were compared with a positive control (solvent well plants) and ranked from 0 to 5. A ranking of 0 indicated that sample well plants grew the same amount as the control. A ranking of 5 indicated no germination.
Cu 36l5
Manufactured by Percival
The CU-36L5 is a laboratory equipment product. It is designed for precision temperature control and measurement. The device features advanced temperature regulation capabilities to ensure accurate and stable environmental conditions for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
11 protocols using cu 36l5
1
Evaluating Allelopathic Effects on Lettuce and Bentgrass
2
Arabidopsis Seed Germination and Growth Assays
3
Arabidopsis Seedling Chromatin-Bound RNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Arabidopsis thaliana (Col-0) seeds were sterilized in 10% sodium hypochlorite for 10 min and rinsed by sterile distilled water 3 to 4 times before being sown on 1/2 MS media. Seeds were stratified at 4 °C for 3 days and transferred to a growth chamber (Percival CU-36L5), 16 h light/8 h dark, 22 °C. 10-day-old seedlings were collected and snap-frozen in liquid N2 for the following chromatin-bound RNAs (CB-RNAs) extraction.
4
Arabidopsis Seedling Assay for HSL Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
See Supplementary Fig. 1a . Seeds were surface sterilized with 70% ethanol for 1–2 min, followed by 10% bleach for 10 min, and then rinsed 5 times with water. They were then suspended in 300 µL of 0.1% agar (Sigma, A7921) and sown at 1 cm intervals onto square Petri dishes (Fisherbrand, FB0875711A) of half strength Murashige and Skoog (Sigma, M5519) medium with 1% sucrose, 0.8% agar (Sigma, A7921) and adjusted to pH 5.7 with KOH (MS medium). The plates were placed in the dark at 4 °C for a 3-day striation period before being moved to a growth chamber (Percival Scientific, CU-36L5) and grown for 7–12 days. In a tissue hood, individual wells of 24-well plates (Falcon, 353047) were filled with 1 mL MS medium. HSLs were added to appropriate wells. Finally, plants were carefully lifted from the agar plates with forceps and moved to individual wells such that the roots were entirely submerged. Each plate was covered with a lid, sealed with MicroporeTM tape, and returned to the growth chamber. After 24 h, plates were taken from the growth chamber for imaging.
5
Arabidopsis T-DNA Insertion Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following T-DNA insertion lines were obtained from the Arabidopsis Biological Resource Center (ABRC, Ohio State University): bbx18-2 (SALK_061956), bbx19-1 (SALK_088902), bbx19-2 (SALK_087493), bbx19-3 (SALK_032997), bbx20-1 (CS878932), bbx21-2 (SALK_105390), bbx22-1 (SALK_105367), bbx23-1 (SALK_053389), bbx24-1 (SALK_067473), and bbx25-3 (CS2103310). The cca1-1 lhy-20 double mutant, in which cca1-1 is in a Ws background (Green and Tobin, 1999 (link)), was created by backcrossing six times with lhy-20 in a Col-0 background (Michael et al., 2003 (link)). toc1-101 was a gift from Peter Quail (Kikis et al., 2005 (link)). Arabidopsis seeds were sterilized in 20% bleach before being placed on 1/2 Murashige and Skoog (MS) medium (M524, PhytoTechnology Laboratories) plus 2% sucrose, and then stratified for 3 days at 4°C in the dark. Plants were grown under a 12:12 LD cycle (white light, 70 μmol m−2 s−1) at 22°C in a growth chamber (Percival CU-36L5).
6
Arabidopsis dig6 Mutant Growth Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Arabidopsis dig6 mutant was isolated for its reduced lateral root growth (Xiong et al., 2006 (link)) from an M2 population generated by ethyl methane sulphonate (EMS) mutagenized Arabidopsis in the Columbia glabrous 1 (Col-gl1) background. Seeds of T-DNA insertion lines and auxin transporter::GFP reporter lines were obtained from the Arabidopsis Biological Resource Center. Seeds were surface sterilized with a 50% bleach solution for 5min, washed with water five times and then planted on half-strength Murashige and Skoog (MS) agar-medium plates. The plates were kept at 4 °C in darkness for 2–4 d and then moved to a growth chamber (CU36-L5, Percival Scientific) at 21 °C under a photoperiod of 16h light and 8h darkness (long-day conditions) or a photoperiod of 8h light and 16h darkness (short-day conditions). For morphological and histological analyses, seedlings were transferred to soil in a growth room under the same growth conditions as in the growth chamber.
7
Seed Sterilization and Bacterial Inoculation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
See Supplementary Fig. 1d . Seeds were surface sterilized with 70% ethanol for 1–2 min, followed by 10% bleach for 10 min, and then rinsed 5 times with water. They were then suspended in 300 µL of 0.1% agar (Sigma, A7921) and kept into the dark at 4 °C for a 3-day striation period. Sterile and non-sterile soil mixtures, prepared as described above, were thoroughly wet with sterile tap water, mixed with a spatula (Cole-Parmer, 17211), and used to fill 10 mL glass beakers (Pyrex, 1000-10). The soil mixture was lightly compressed to provide a firm bed for the seeds. After the striation period, the seeds were incubated for 1 h with P. putida WT or P. putida pTT337 grown overnight at 30 °C in LB medium at 900 rpm (INFORS HT, Multitron Pro). The seeds were then transferred to soil (either sterile or non-sterile). The glass beakers were covered with Saran wrap, held into place with elastic bands and 3 puncture holes were made with the tip of sterile forceps to allow for aeration in the beaker. Plants were then incubated for 20 days in the growth chamber (Percival Scientific, CU-36L5) and they were watered with autoclaved MilliQ water supplemented with 100 µM of p-coumarate at day 5 and day 15. After 20 days, the plants roots were cleaned with tap water to remove the soil for imaging.
8
Arabidopsis Seed Sterilization and Growth
9
Arabidopsis Seed Sterilization and Growth
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seeds were sterilized for 10 min in 20% bleach and then rinsed four times in sterile-deionized water. Sterilized seeds were grown horizontally on 0.3% Phytagel (Sigma) or vertically on 0.6% Phytagel medium containing 1/2 MS nutrients (PhytoTech), 1% sucrose, pH5.7, and kept at 4–8°C for 3 days. For ABA related analysis, 1 g/L MES buffer anhydrous (Amresco, E183) was added before autoclaving to prevent acidification of ABA. ABA (Sigma, A1049) was added to the cooled, autoclaved medium prior to pouring plates. Seedlings were grown in a Percival CU36L5 incubator at 23°C under a 16-h light/8-h dark photoperiod. Seedlings were grown vertically for 4 d before transfer to medium with or without the indicated concentrations of ABA or mannitol. Arabidopsis plants were grown in soil (Fafard Super Fine Germination Mix) in a growth room at 22°C under a 60%–65% relative humidity and a 16-h light/8-h dark photoperiod. For pyl decuple, hendecuple, duodecuple mutants and snrk2.2/3/6 triple mutant, seedlings were partially covered with stackable plastic covers to maintain 80%–90% humidity (Figure S1E ) and were watered every two days.
10
Arabidopsis Developmental Transitions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Arabidopsis Col-0, bmi1abc (Yang et al. 2013) (link), and clf swn (Lafos et al. 2011) mutants and BMI1B-FLAG bmi1b transgenic plants (Wang et al. 2014) (link) were grown in a growth chamber (CU36L5, Percival Scientific) under cool white light (Philips, 100 μmol m -2 s -1 ) in long-day conditions (16 h of light/8 h of dark) at 22 °C on half-strength MS agar plates containing 1% sucrose and 0.7% agar.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!