Vascular endothelial growth factor a 165

The following agents were obtained from commercial sources: vascular endothelial growth factor‐A 165 (Merck Millipore, Billerica, MA, USA); anti‐phospho‐VEGFR‐2 (Y951) (Abcam, Cambridge, UK); anti‐phospho‐p70^S6K (T421/S424), anti‐phospho‐Akt (S473), anti‐phospho‐ERK (T202/Y204), anti‐phospho‐p38^MAPK (T180/Y182), anti‐phospho‐pRb (S780) and anti‐phospho‐pRb (S807/S811) (Cell Signaling, Beverly, MA, USA); anti‐phospho‐tyrosine (BD Biosciences, Bedford, MA, USA); anti‐VEGFR‐2, anti‐vascular endothelial (VE)‐cadherin, anti‐integrin β1, anti‐ILK, anti‐p70^S6K, anti‐Akt, anti‐ERK, anti‐p38^MAPK, anti‐Cdk2, anti‐Cdk4, anti‐cyclin D, anti‐cyclin E, anti‐actin antibodies and mouse and rabbit IgG‐horseradish peroxidase conjugates (Santa Cruz Biotechnology, Santa Cruz, CA, USA); Alexa Fluor 488–conjugated goat anti‐mouse IgG (Life Technologies, Grand Island, NY, USA).

GV1001, a human telomerase-derived 16-mer peptide, was provided by GemVax-KAEL (Seongnam, Republic of Korea). The following agents were obtained from commercial sources: vascular endothelial growth factor-A 165 (Merck Millipore, Billerica, MA, USA); anti-phospho-VEGFR-2 (Y1175), anti-phospho-MEK (S217/S221), anti-MEK, anti-phospho-Src (Y416), anti-Src, anti-phospho-p70^S6K (T421/S424), anti-phospho-Akt (S473), anti-phospho-ERK (T202/Y204), anti-phospho-pRb (S780), and anti-phospho-pRb (S807/S811) (Cell Signaling Technology, Beverly, MA, USA); fibroblast growth factor-2 (FGF-2), anti-phosphotyrosine, anti-phospho-FAK(Y397), anti-FAK, anti-β-catenin, and anti-p120-catenin (BD Biosciences, Bedford, MA, USA); anti-vascular endothelial (VE)-cadherin, anti-VEGFR-2, anti-p70^S6K, anti-Akt, anti-ERK, anti-Cdk2, anti-Cdk4, anti-cyclin D, anti-cyclin E, anti-p27^kip1, anti-actin antibodies, and mouse and rabbit IgG-horseradish peroxidase conjugates (Santa Cruz Biotechnology, Santa Cruz, CA, USA); goat anti-mouse IgG-Alexa Fluor 488 conjugate (Thermo Fisher Scientific Co., Waltham, MA, USA).