Monomers HPA, MA, BA, HA, and CHA were purchased from Polysciences, VWR, Sigma-Aldrich, and Fisher Scientific, respectively. Chain transfer agent (CTA) 4-cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl]pentanoic acid was purchased from Sigma Aldrich while the initiator ZnTPP was from Tokyo Chemical Industry Co., Ltd. Prior to handling, all monomers were deinhibited by pipetting over a column of inhibitor removal beads (Sigma-Aldrich). Monomer aliquots were made at 2 M, CTA aliquots at 50 mM, and ZnTPP aliquots at 2 mM in DMSO. The industry standard polymers used as controls were HPMCAS, HPMC, and vinyl pyrrolidone-vinyl acetate copolymer (PVP/VA). Three water soluble grades of HPMCAS (Shin-Etsu AQOAT®) were utilized: LF, MF, and HF. HPMC (Shin-Etsu PHARMACOAT®) 603 grade was also used. PVP/VA was sourced from BASF. Probucol was purchased from Combi-Blocks (98% purity).
Pvp va
Manufactured by BASF
Sourced in Germany
PVP/VA is a polymer product manufactured by BASF. It functions as a binder and film-forming agent. The core properties of PVP/VA include excellent adhesion, compatibility, and solubility in various solvents.
Automatically generated - may contain errors
Lab products found in correlation
Inhibitor removal beads, by Merck Group (1 mentions)
Probucol, by Combi-Blocks (1 mentions)
Sodium hydroxide (naoh), by Carl Roth (1 mentions)
Potassium dihydrogen phosphate, by Carl Roth (1 mentions)
Tunel apoptosis assay kit, by Beyotime (1 mentions)
Dihydroethidium dhe, by Keygen Biotech (1 mentions)
Calcein am pi, by Keygen Biotech (1 mentions)
Isoflurane, by Sinopharm (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Coumarin 6, by Merck Group (1 mentions)
Methanol, by Tedia (1 mentions)
Acetonitrile, by Tedia (1 mentions)
Soluplus, by BASF (1 mentions)
4 protocols using pvp va
1
Synthesis and Characterization of Functional Polymers
2
Formulation and Characterization of Polymorphic Drug Tablets
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PCM (polymorphic form I) was a generous gift from STADA (Bad Vilbel, Germany). The polymers PVP/VA (M ~ 45,000 g/mol), PVP 90 (M = 1,000,000–1,500,000 g/mol), and PVP 30 (M = 44,000–54,000 g/mol) were kindly donated by BASF (Ludwigshafen, Germany), while PVP 12 (M = 2000–3000 g/mol) was purchased from Carl Roth (Karlsruhe, Germany). Microcrystalline cellulose 200 (MCC 200) was used as an excipient for the tablet blends and was kindly donated by JRS Pharma (Rosenberg, Germany). Magnesium stearate was purchased from Caelo (Hilden, Germany). Methanol was purchased from Carl Roth (Karlsruhe, Germany), and doubly distilled water was used. Phosphate buffer (pH 5.8) was used for the drug release studies. Potassium dihydrogen phosphate and sodium hydroxide were obtained from Carl Roth (Karlsruhe, Germany).
3
Multifunctional Nanoplatform for Cancer Therapy
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CDDP, IR820, Hoechst 33342, methylthiazolyldiphenyl-tetrazolium bromide (MTT) and 1,3-diphenylisobenzofuran (DPBF) were purchased from Aladdin Company (Shanghai, China). PVPVA (trade name Kollidon VA64) was gifted by Basf Corporation (Shanghai, China). The polydimethylsiloxane (PDMS) MN molds were bought from Micropoint Technologies Pte Ltd (Singapore). The dihydroethidium (DHE) and the live/dead assay kit (Calcein AM/PI) were purchased from KeyGen Biotech (Nanjing, China). The total reactive oxygen species assay kit (DCFH-DA), the active caspase-3 assay kit (GreenNuc) and the TUNEL apoptosis assay kit were obtained from Beyotime Biotechnology (Shanghai, China). The anti-Ki67 and anti-cleaved caspase 3 primary antibodies were purchased from Servicebio Technology (Wuhan, China). Other regents were analytical grade.
4
Silybin-Loaded Nanoparticle Formulation
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Silybin was donated by Tianjin Tasly Pharmaceuticals Co., Ltd. (Tianjin, China). Soluplus and PVPVA were kindly donated by BASF (Ludwigshafen, Germany). Coumarin-6 was purchased from Sigma–Aldrich (Saint Louis, Missouri, USA). RPMI 1640 medium and 0.25% trypsin were obtained from Invitrogen (Ontario, CA, USA). Fetal bovine serum was purchased from Sijiqing Biological Engineering Materials Co., Ltd. (Zhejiang, China). Heparin sodium (>150 IU/mg) and isoflurane were obtained from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Acetonitrile and methanol of HPLC grade were purchased from Tedia Company, Inc. (Fairfield, OH, USA). A Milli-Q water purification system was used to prepare water (Millipore Corp., Billerica, MA, USA). All other chemicals used were of analytical reagent grade and were purchased locally.
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