Poly 2 ethyl 2 oxazoline

Methyl p-toluenesulfonate, ethylenediamine, and super dehydrated acetonitrile were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 2-Ethyl-2-oxazoline was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (WSC) was purchased from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). 4-Dimethylaminopyridine (DMAP) and 2 mol/l-hydrochloric acid were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Poly(2-Ethyl-2-oxazoline) (molecular weight: 25, 50, and 200 kDa) was purchased from Sigma-Aldrich Japan (Tokyo, Japan). 1H-Benz[e]indolium, 2-[7-[3-(5-carboxypentyl)-1,3-dihydro-1,1-dimethyl-2H-benz[e]indol-2-ylidene]-1,3,5-heptatrien-1-yl]-1,1-dimethyl-3-(4-sulfobutyl), inner salt (ICCA) was synthesized according to previously reported method32 (link). All other chemicals of highest purity available were used.

Poly(2-ethyl-2-oxazoline) (PEOZ), 50 kDa (polydispersity index, PDI 3-4); poly(N-vinyl pyrrolidone) (PVP), 55 kDa (K-value 30); and buffer tablets, pH 6.8 were from Sigma-Aldrich (Gillingham, UK). Poly(2-methyl-2-oxazoline) (PMOZ), poly(2-n-propyl-2-oxazoline) (PnPOZ), and poly(2-isopropyl-2-oxazoline) (PiPOZ) were synthesized according to our previously reported procedure [29 (link)]. Ibuprofen (IB) was from Tokyo Chemistry Industry (Japan). N, N-dimethylacetamide (DMA) was from Fisher Scientific (Loughborough, UK).

Poly(ethylene glycol) (PEG, Mw ~8000), Poly(vinyl alcohol) (PVA, Mw: 89,000–98,000, 99% hydrolyzed), Poly(2-ethyl-2-oxazoline) (PEtOx, Mw~50, 000), sodium citrate, Poly(ethylene glycol) diacrylate (PEGDA, Mn~575), sodium alginate, 2-Hydroxy-4′-(2-hydroxyethoxy)−2-methylpropiophenone (Irgacure 2959), N,N′-methylenebisacrylamide (MBAA), were purchased from Sigma-Aldrich. Dextran (DEX, Mw ~10,000, 40,000, and 500,000) and Nile red were purchased from Macklin. Acrylamide and lithium phenyl-2,4,6 trimethylbenzoylphosphinate (LAP) were purchased from TCI. Rhodamine 6 G, Fluorescein isothiocyanate (FITC) labeled Poly-l-lysine (FITC-PLL, Mw ~30,000–70,000), and Fluorescein isothiocyanate–dextran (FITC-dextran, Mw ~40, 000) were purchased from Sigma-Aldrich. Rhodamine-labeled PEG (Rh-PEG, Mw ~40,000) was purchased from Aladdin. Nucleic acid sequences of Cy5-DNA were synthesized by Integrated DNA Technology (IDT). Calcein was purchased from J&K scientific. Millipore Milli-Q water (18.2 MΩ, pH = 7) was used in all experiments. All materials were used as received without further purification.