Pg-LPS was bought from Sigma (St. Louis, MO, USA). Rabbit RIP1, MLKL and beta-actin antibody was from Proteintech (USA), and mouse RIP3 antibody was from Santa (USA). Real-time PCR primer COL-1, OCN, RUNX-2, BSP, TNF-α, IL-1β, IL-18 and GAPDH were from Genecopoeia (USA), SYBR mixture was from Takara (Japan), RT Master Mix was from Takara (Japan). Inhibitor of Nec-1 (ab141053) was from Abcam (USA). Annexin V-FITC/PI: Cell apoptosis detection kit was from Bestbio Company (China). Nude mice were bought from Animal Center of the Fourth Military Medical University Animal Center.
Real time pcr primer col 1
Manufactured by GeneCopoeia
Sourced in United States
The Real-time PCR primer COL-1 is a laboratory equipment product designed for use in real-time PCR (polymerase chain reaction) experiments. It serves the core function of amplifying and detecting target DNA sequences in a quantitative manner.
Automatically generated - may contain errors
Lab products found in correlation
Runx 2, by GeneCopoeia (2 mentions)
Il 18, by GeneCopoeia (2 mentions)
Il 1β, by GeneCopoeia (2 mentions)
Tnf α, by GeneCopoeia (2 mentions)
Gapdh, by GeneCopoeia (2 mentions)
Rt master mix, by Takara Bio (2 mentions)
Annexin 5 fitc pi cell apoptosis detection kit, by BestBio (2 mentions)
Sybr mixture, by Takara Bio (1 mentions)
Pg lps, by Merck Group (1 mentions)
Beta actin antibody, by Proteintech (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Sybr mixture, by CWBIO (1 mentions)
2 protocols using real time pcr primer col 1
1
Necroptosis Regulation in Bone Cells
2
Molecular Mechanisms of Cell Death
Check if the same lab product or an alternative is used in the 5 most similar protocols
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LPS was bought from Sigma (St. Louis, MO, USA). Rabbit RIP3, RIP1, MLKL, and β-action antibody were from Proteintech (Chicago, USA). Real-time PCR primer COL1, OCN, RUNX-2, BSP, TNF-α, IL-1β, IL-18, and GAPDH were from Genecopoeia (USA), and SYBR mixture was from CWBIO Company (China). RT Master Mix was from Takara (Japan). Inhibitor Z-VAD-fmk (ab120382) was from Abcam and GSK’872(2673-5) was bought from BIOVISION. The Annexin V-FITC/PI cell apoptosis detection kit was from Bestbio Company (China). Anti-vimentin antibody (ab8978), Anti-pan Cytokeratin antibody (ab7753), and Alexa Fluor 647(ab150171) were bought from Abcam and Goat Anti-Mouse IgG and Cy3 was bought from Zhuangzhibio (Xi’an, China).
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