RAW 264.7 macrophages and H9C2 cells were purchased from China Infrastructure of Cell Line Resources (Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences). They were incubated with Dulbecco’s modified Eagle’s medium (DMEM; Corning, United States) supplemented with 10% fetal bovine serum (FBS, Corning, United States), penicillin (100 U/mL; Corning, United States) and streptomycin (100 μg/mL; Corning, United States) at 37°C in a humidified atmosphere of 5% CO2.
H9c2 cells
Manufactured by China Infrastructure of Cell Line Resources
Sourced in China
H9C2 cells are a cell line derived from rat embryonic cardiomyocytes. They are commonly used in in vitro studies to model cardiac muscle cells and investigate various aspects of cardiac physiology and pathology.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Corning (2 mentions)
Dulbecco modified eagle medium (dmem), by Corning (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Raw 264.7 macrophages, by China Infrastructure of Cell Line Resources (1 mentions)
Penicillin, by Corning (1 mentions)
Streptomycin, by Corning (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
H9c2 cells, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
H9c2 cells, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Humidified incubator, by Thermo Fisher Scientific (1 mentions)
5 protocols using h9c2 cells
1
Cell Culture Protocols for RAW 264.7 and H9C2 Cells
2
Cardiomyocyte Viability Assay Using H9c2 Cells
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H9c2 cells, a cardiomyocyte cell line, were obtained from China Infrastructure of Cell Line Resources. H9c2 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA) at 37 °C with 5% CO2 for monolayer confluency of about 80%~90%. Cells were harvested with 0.25% Trypsin-EDTA (Gibco, Grand Island, NY, USA) and seeded (8000 cells/well) into 96-well plates for 24 h, then treated with DOX (Yuanye, Shanghai, China) for 24 h at desired concentrations.
3
H9C2 Cell Viability Assay with PC
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H9C2 cells were obtained from China Infrastructure of Cell Line Resources and maintained in Dulbecco's modified Eagle's medium, containing 100 mg/ml streptomycin, 100 U/ml penicillin, and fetal bovine serum (10%) at 37°C with 5% CO2 and 95% air. We changed the medium daily until the H9C2 cells were at 80-90% confluence. H9C2 cells (1 × 104 cell/well) were inoculated into a 96-well plate and then pretreated with different concentrations of PC (50, 100, 200, and 400 μg/ml) for 2 h and then coincubated with angiotensin II (1 μM) for 48 h. Then, a CCK8 Kit was used to measure the cell viability. Briefly, 10 μl of CCK-8 was added to each pore for incubation of 2 h. The absorbance was measured at 450 nm using the microplate reader.
4
Evaluating RAF Effects on H9c2 Cells
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H9c2 cells (China Infrastructure of Cell Line Resources, Beijing, China) was cultured under the condition of 37 °C with 5% CO2 and the medium was DMEM (Corning, Manassas, VA, USA) mixed with 10% FBS (Corning, Manassas, VA, USA). DMSO (Sigma, USA) was used to dissolve RAF and the initial concentration was 40 mg/mL. Firstly, the H9c2 cells were seeded in 96-well plates with the density of 8 × 103 cells/well for 24 h before incubating with RAF (1.25, 2.5, 5, 10, 20, 40, 80, 160, and 320 μg/mL) for 24 h. Secondly, we put the 96-well plates into the hypoxia box and added RAF at the concentrations of 0.63, 1.25, 2.5, 5, and 10 μg/mL for 8 h. Control group was cultured without the hypoxia box and other conditions were the same. Finally, each well was added 100 μL CCK-8 and incubated for 2 h, then a microplate reader was used to measure the OD value of the plates at 450 nm.
5
Culturing H9C2 Cardiac Cells
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H9C2 cells were purchased from China Infrastructure of Cell Line Resources (Chinese Academy of Medical Sciences, China). The cells were cultured in dulbecco’s modified eagle medium (DMEM) with 10% fetal bovine serum (FBS) and 100 mg/ml streptomycin and 100 U/mL penicillin at 37°C in a humidified incubator (Thermo, NYC, United States).
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