1260 infinity quaternary lc

Manufactured by Agilent Technologies

Sourced in United States, Germany

The Agilent 1260 Infinity Quaternary LC is a liquid chromatography system designed for high-performance liquid chromatography (HPLC) analysis. It features a quaternary solvent delivery system capable of providing precise and accurate solvent blending for a wide range of analytical applications.

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1260 Infinity (552 citations) 1260 Infinity LC (56 citations) 1260 Infinity Quaternary LC System (20 citations) Agilent 1260 Infinity Quaternary LC system (8 citations) Agilent 1260 Infinity Quaternary LC (8 citations) Agilent 1260 Infinity Quaternary LC apparatus (4 citations) Agilent 1260 Infinity LC System with Quaternary Pump and UV detector (2 citations) 1260 Infinity Quaternary LC apparatus (2 citations) 1260 Infinity II Quaternary LC instrument (2 citations) 1260 Infinity Bio-inert Quaternary LC system (2 citations)

12 protocols using 1260 infinity quaternary lc

General Organic Chemistry Techniques

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All reagents were purchased from
commercial suppliers and used without further purification. Reactions
were carried out in 4 mL screw neck glass vials furnished with screw
caps equipped with poly(tetrafluoroethylene) (PTFE)/rubber septa,
and stir bars under ambient atmosphere unless otherwise noted. Silica
gel 60 Å (40–60 μm, 230–400 mesh) was used
for column chromatography. All NMR spectra were recorded in CDCl₃ using a Bruker AVANCE II 400 MHz or Bruker Avance 500 MHz.
Chemical shifts are given in ppm relative to the residual solvent
peak (¹H NMR: CDCl₃ δ 7.26, ¹³C NMR: CDCl₃ δ 77.16) with multiplicity (br = broad,
s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet),
coupling constants (in hertz), and integration. Kinetic data was analyzed
by Agilent 1260 Infinity Quaternary LC (Eclipse Plus 18C column, 3.5
μm, 4.6 × 100 mm²; UV detector, 265 nm) with
a gradient of acetonitrile and 0.1% formic acid in Milli-Q water at
a flow rate of 1 mL/min. The analytes were calibrated using a five-point
calibration curve with threefold dilution between each sample in the
series. HPLC with a chiral stationary phase was performed on an Agilent
1100 series instrument. High-resolution mass spectrometry analyses
were performed by Thermo Scientific Q Exactive HF Hybrid Quadrupole-Orbitrap
HESI or Bruker microTOF ESI, and low-resolution mass analyses by Bruker
Daltonics amaZon speed no 06052 ESI.

Villo P., Dalla-Santa O., Szabó Z, & Lundberg H. (2020). Kinetic Analysis as an Optimization Tool for Catalytic Esterification with a Moisture-Tolerant Zirconium Complex. The Journal of Organic Chemistry, 85(11), 6959-6969.

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Quantification of Carbamazepine by HPLC

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Carbamazepine concentrations were quantified by HPLC (Agilent 1260 Infinity Quaternary LC, Santa Clara, CA, USA) with an ultraviolet light (UV) detector using a 4.6 × 50 mm ODS Hypersil C18 column (Thermo Scientific) with injection volume of 10 µL, 40% acetonitrile and 60% HPLC grade water as eluent, flow rate of 1 mL/min, and wavelength of UV absorbance of 220 nm.

Cai N, & Larese-Casanova P. (2020). Facile Synthesis and Reuse of Magnetic Black Carbon Magnetite (BC-Mag) for Fast Carbamazepine Removal from Water. Nanomaterials, 10(2), 213.

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Chromatographic Analysis of Compounds

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Chromatographic analysis was carried out using Agilent 1260 Infinity Quaternary LC, supplied with Agilent 1260 Infinity; Quaternary Pump (G1311B), and Diode Array Detector (G1315D, VL) coupled to Agilent Open LAB Chem Station B.04.03 software. Analytes were separated on Agilent Zorbax Eclipse XDB-C18 (250 mm× 4.6 mm i.d., 5 μm particle diameter) protected with Agilent Zorbax XDB-C18 pre-column (Agilent Technologies, Palo Alto, CA, USA). The flow rate of 1.0 mL/min and the UV detector was set at 320 nm. Separation was performed with 0.1% trifluoroacetic acid in acetonitrile (A) and 0.1% trifluoroacetic acid in water (B) and gradient elution program at a flow rate of 1 ml/min starting with 20% A in B for 5 min, 20–30% A/B in 5 min, 30–50% A in B in 5 min, 50–100% A in B in 10 min followed by washing with 100% A for 5 min.

Fayez N., Khalil W., Abdel-Sattar E, & Abdel-Fattah A.F. (2024). Involvement of TNFα, IL-1β, COX-2 and NO in the anti-inflammatory activity of Tamarix aphylla in Wistar albino rats: an in-vivo and in-vitro study. BMC Complementary Medicine and Therapies, 24, 57.

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HPLC Analysis of Honey Polyphenols

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HPLC analysis was carried out to measure the polyphenolic content of Shaoka and Manuka honey by an Agilent 1260 Infinity Quaternary LC, USA. The separation was executed using an Eclipse plus c18 column (4.6 mm × 250 mm i.d., 5 μm). The movable phase is composed of water (A) and 0.02% trifluoroacetic acid in acetonitrile (B) at a flow rate of 1 mL/min. This mobile stage was programmed consecutively in a linear gradient as follows: 0 min (80% A); 0–5 min (80% A); 5–8 min (40% A); 8–12 min (50% A); 12–14 min (80% A), and 14–16 min (80% A). Then, 10 µl of each sample solution was injected into the multi-wavelength detector at 280 nm. The temperature of the column was maintained at 35 °C (Chan et al., 2013 (link)). The phenolic compounds were identified by comparing the retention time and UV-spectra with 14 standard phenolic compounds selected for comparison based on their common presence in honey.

, & Halawani E.M. (2021). Potential effects of Saudi Shaoka (Fagonia bruguieri) honey against multi-drug-resistant bacteria and cancer cells in comparison to Manuka honey. Saudi Journal of Biological Sciences, 28(12), 7379-7389.

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HPLC analysis of Lamotrigine

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HPLC system was Agilent 1260 Infinity Quaternary LC (USA) with a UV–Vis Detector (USA). Zorbax C18 column (150 × 4.6 mm i.d.) was applied as stationary phase. The software used was Agilent Lab Advisor (USA). A mixture of KH₂PO₄ (50 mM) and methanol (61:39) was used for separation at 1.0 ml/min. The column temperature was 37 °C. LTG was monitored at 210 nm for 10 min, and LTG retention time was 6.10 min (Jin et al. 2019 (link)).

Harby S.A., Khalil N.A., El-Sayed N.S., Thabet E.H., Saleh S.R, & Fathelbab M.H. (2023). Implications of BCRP modulation on PTZ-induced seizures in mice: Role of ko143 and metformin as adjuvants to lamotrigine. Naunyn-Schmiedeberg's Archives of Pharmacology, 396(10), 2627-2636.

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RP-HPLC Separation of Biomolecules

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RP-HPLC separation was performed on Agilent 1260 Infinity Quaternary LC coupled to a diode array detector (DAD). Best peak resolution was demonstrated using Agilent AdvanceBio RP-mAb Diphenyl, 2.1 × 100 mm column, 0.5 mL/min flow rate, with a column temperature of 60 °C and a step wise gradient (3 min washing at 35% B followed by 35% B to 39% B over 16 min). The eluted peaks were detected at 280 nm.

Sharma V.K., Misra B., McManus K.T., Avula S., Nellaiappan K., Caskey M., Horowitz J., Nussenzweig M.C., Seaman M.S., Javeri I, & Dey A.K. (2020). Characterization of Co-Formulated High-Concentration Broadly Neutralizing Anti-HIV-1 Monoclonal Antibodies for Subcutaneous Administration. Antibodies, 9(3), 36.

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HPLC Analysis of Organic Compounds

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Using Agilent 1260 Infinity Quaternary LC, equipped with Agilent 1260 Infinity; Quaternary Pump (G1311B), and Diode Array Detector (G1315D, VL) coupled to Agilent Open LAB Chem Station B.04.03 software, chromatographic analysis was carried out. Analytes were separated on Agilent Zorbax Eclipse XDB-C18 (250 mm × 4.6 mm i.d., 5_mparticle diameter) protected with Agilent Zorbax XDB-C18 pre-column (Agilent Technologies, Palo Alto, CA, USA). The flow rate of 1.0 mL/min and the UV detector was set at 280 nm. Separation was carried out with acetonitrile (A) and 0.1% trifluoroacetic acid in water (B) and gradient elution program at a flow rate of 1 ml/min starting with 20% A in B for 5 min, 20–30% A/B in 5 min, 30–50% A in B in 5 min, 50–100% A in B in 10 min followed by washing with 100% A for 5 min.

Fayez N., Khalil W., Abdel-Sattar E, & Abdel-Fattah A.F. (2023). In vitro and in vivo assessment of the anti-inflammatory activity of olive leaf extract in rats. Inflammopharmacology, 31(3), 1529-1538.

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Comprehensive Spectroscopic Characterization

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NMR spectra were recorded on a Bruker AVANCE 500 MHz or 600 MHz (Bruker Biospin, Falländen, Switzerland), as required. NMR spectra were obtained dissolving samples in CDCl₃ (99.9%) and chemical shifts are reported relative to solvent (δ_H 7.26 and δ_C 77.0 ppm). Bruker AVANCE 600 MHz instrument is equipped with a 5 mm TCI inverse detection cryoprobe (Bruker Biospin, Falländen, Switzerland). Standard Bruker NMR pulse sequences were utilized. Optical rotations were measured in CHCl₃ on a PerkinElmer 241 polarimeter (Waltham, MA, USA) by using a Na lamp. HR-ESI-MS data were obtained on a Waters LCT Premier XE Micromass (Manchester, UK) and VG-AutoSpec Micromass spectrometers (Manchester, UK), respectively. IR spectra were recorded on a Bruker IFS66/S (Ettlingen, Germany) equipped with an ATR accessory using CHCl₃ solutions. EnSpire ^® Multimode Reader (Perkin Elmer, Waltham, MA, USA) using absorbance values of alamarBlue^® reagent (Bio-Rad Laboratories, Oxford, UK). HPLC (high performance liquid chromatography) separations were carried out with an Agilent 1260 Infinity Quaternary LC equipped with a Diode Array Detector (Waldbronn, Germany). TLC (Thin layer chromatography) (Merck, Darmstadt, Germany) was visualized by UV light (254 nm) and spraying with cobalt chloride reagent (2% in sulfuric acid, 10%) and heating.

Cartuche L., Reyes-Batlle M., Sifaoui I., Arberas-Jiménez I., Piñero J.E., Fernández J.J., Lorenzo-Morales J, & Díaz-Marrero A.R. (2019). Antiamoebic Activities of Indolocarbazole Metabolites Isolated from Streptomyces sanyensis Cultures. Marine Drugs, 17(10), 588.

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Photocatalytic Degradation of Phenol

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Phenol was used as model pollutant in water (C6H5OH, 50 ppm). Its photodecomposition was studied in a 10-mm light path quartz cell reactor containing 3.5 mL of the phenol solution and 1 g L -1 of photocatalyst. The photocatalyst was dispersed in the solution by sonication and kept under stirring for 10 min under dark conditions to reach the equilibrium between adsorption and desorption. Then, the solutions were irradiated with a xenon lamp (Oriel 300W) and a cut-off filter (AM-32603-1, LOT-Oriel) (λ > 450 nm) was used. The photocatalytic tests were conducted under oxygen bubbling at a fixed rate flow.
Sampled aliquots of 0.5 mL were taken from the irradiated suspensions at different time intervals. The catalyst powders were separated from the solutions by centrifugation.
The solutions were analyzed by High Performance Liquid Chromatography (HPLC, Agilent 1260 infinity quaternary LC) equipped with a UV-detector set at 260 nm for phenol analysis. An isocratic mobile phase consisted of 80% H2O and 20% acetonitrile (ACN) for elution at 1 mL min -1 flow rate.

Méndez-Medrano M.G., Kowalska E., Ohtani B., Bahena Uribe D., Colbeau-Justin C., Rau S., Rodríguez-López J.L, & Remita H. (2020). Heterojunction of CuO nanoclusters with TiO₂ for photo-oxidation of organic compounds and for hydrogen production. The Journal of chemical physics, 153(3).

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Characterization of Organic Compounds

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Optical rotations were measured in CH>₂Cl₂ on a PerkinElmer 241 polarimeter (Waltham, MA, USA) by using a Na lamp. NMR spectra were recorded on a Bruker AVANCE 500 MHz or 600 MHz (Bruker Biospin, Falländen, Switzerland), as required. NMR spectra were obtained dissolving samples in CDCl3 (99.9%) and chemical shifts are reported relative to solvent (δH 7.26 and δC 77.0 ppm). Bruker AVANCE 600 MHz instrument is equipped with a 5 mm TCI inverse detection cryoprobe (Bruker Biospin, Falländen, Switzerland). Standard Bruker NMR pulse sequences were utilized. HR-ESI-MS data were obtained on an Waters LCT Premier XE Micromass (Manchester, UK) and VG -AutoSpec Micromass spectrometers (Manchester, UK), respectively. IR spectra were recorded on a Bruker IFS66/S (Ettlingen, Germany) equipped with an ATR accessory using CH₂Cl₂ solutions. EnSpire^® Multimode Reader (Perkin Elmer, Waltham, MA, USA) using absorbance values of Alamar Blue^® reagent (Bio-Rad Laboratories, Oxford, UK). HPLC (High performance liquid chromatography) separations were carried out with an Agilent 1260 Infinity Quaternary LC equipped with a Diode Array Detector (Waldbronn, Germany). TLC (Thin layer chromatography) (Merck, Darmstadt, Germany) was visualized by UV light (254 nm) and spraying with cobalt chloride reagent (2% in sulfuric acid, 10%) and heating.

Chiboub O., Sifaoui I., Lorenzo-Morales J., Abderrabba M., Mejri M., Fernández J.J., Piñero J.E, & Díaz-Marrero A.R. (2019). Spiralyde A, an Antikinetoplastid Dolabellane from the Brown Alga Dictyota spiralis. Marine Drugs, 17(3), 192.

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