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Bacteroides vulgatus

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Bacteroides vulgatus is a Gram-negative anaerobic bacterium that can be used in laboratory settings. It is part of the normal human gut microbiome.

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Lab products found in correlation

Enterococcus faecalis, by American Type Culture Collection (3 mentions) Bacillus cereus, by American Type Culture Collection (2 mentions) Escherichia coli, by American Type Culture Collection (2 mentions) Pseudomonas aeruginosa, by American Type Culture Collection (2 mentions) Staphylococcus aureus, by American Type Culture Collection (2 mentions) Bacteroides fragilis, by American Type Culture Collection (1 mentions) Clostridium scindens, by American Type Culture Collection (1 mentions) Bacteroides uniformis, by American Type Culture Collection (1 mentions) Bacteroides caccae, by American Type Culture Collection (1 mentions) Parabacteroides distasonis, by American Type Culture Collection (1 mentions) Bacteroides ovatus, by American Type Culture Collection (1 mentions) Bifidobacterium adolescentis, by American Type Culture Collection (1 mentions) Staphylococcus epidermidis, by American Type Culture Collection (1 mentions) Acinetobacter baumannii, by American Type Culture Collection (1 mentions) Lactobacillus gasseri, by American Type Culture Collection (1 mentions) Streptococcus mutans, by American Type Culture Collection (1 mentions) Helicobacter pylori, by American Type Culture Collection (1 mentions) Cutibacterium acnes, by American Type Culture Collection (1 mentions) Streptococcus agalactiae, by American Type Culture Collection (1 mentions) Deinococcus radiodurans, by American Type Culture Collection (1 mentions)

5 protocols using bacteroides vulgatus

1

Comparative Genomic Analysis of Campylobacter concisus

Cited 2 times
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A total of 15 bacterial strains of five species were used in this study, including 11 C. concisus strains and Bacteroides fragilis ATCC 25285, Bacteroides vulgatus ATCC 8482, Enterococcus faecalis ATCC 19433 and Escherichia coli K12 (Bachmann, 1972 (link); Gregory et al., 1978 (link); Moschetti et al., 1998 (link)). Of the 11 C. concisus strains examined, three strains were from patients with CD, four strains from patients with UC, one strain from patient with gastroenteritis and three strains from healthy controls. These 11 C. concisus strains were randomly chosen from C. concisus strains isolated in our previous studies (Zhang et al., 2009 (link), 2010 (link); Mahendran et al., 2011 (link)). C. concisus consists of two genomospecies (GS), among the 11 strains included, five strains were from GS1 and six strains were from GS2 (Mahendran et al., 2015 (link); Chung et al., 2016 (link)). The other bacterial strains were purchased from the American Type Culture Collection (VA, USA). Detailed information of bacterial species and strains used in this study were provided in Table 1.
Liu F., Ma R., Riordan S.M., Grimm M.C., Liu L., Wang Y, & Zhang L. (2017). Azathioprine, Mercaptopurine, and 5-Aminosalicylic Acid Affect the Growth of IBD-Associated Campylobacter Species and Other Enteric Microbes. Frontiers in Microbiology, 8, 527.
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2

Gut Microbiome Bacterial Strains Cultivation

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The following bacterial strains were obtained from the American Type Culture Collection (ATCC): Clostridium difficile ATCC 9689; Clostridium difficile clinical isolates from the ATCC include: BAA-1870 (ribotype 027); BAA-1801 (ribotype 010); BAA-1814 (ribotype 251); Clostridium scindens ATCC 35704; Clostridium sordelli ATCC 9714; Bacteroides vulgatus ATCC 8482. Clostridium scindens VPI 12708 was obtained from the Virginia Tech Anaerobe Laboratory. Bacteria were cultured in peptone-yeast extract-fructose (PYF) medium and growth monitored at 600 nm.
Kang J.D., Myers C.J., Harris S.C., Kakiyama G., Lee I.K., Yun B.S., Matsuzaki K., Furukawa M., Min H.K., Bajaj J.S., Zhou H, & Hylemon P.B. (2018). Bile Acid 7α-Dehydroxylating Gut Bacteria Secrete Antibiotics that Inhibit Clostridium difficile: Role of Secondary Bile Acids. Cell chemical biology, 26(1), 27-34.e4.
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3

Culturing Synthetic Gut Microbiome

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Synthetic community strains were cultured in pre-reduced Mega Medium15 (link) or on EG Agar29 at 37 °C in an anaerobic chamber (Coy Laboratories, Grass Lake, MI, USA) with an atmosphere of 5% hydrogen, 10% CO2 and 85% N2. Synthetic community strains included Bacteroides uniformis ATCC 8492, Bacteroides vulgatus ATCC 8482, Bacteroides thetaiotaomicron VPI-5482, Bacteroides caccae ATCC 43185, Bacteroides ovatus ATCC 8483, Parabacteroides distasonis ATCC 8503, Eubacterium rectale ATCC 33656, Dorea longicatena DSM 13814, Ruminococcus torques ATCC 27756, and Clostridium scindens ATCC 35704. All strains were obtained from the American Type Culture Collection (ATCC), except D. longicatena DSM 13814, which was obtained from The Leibniz Institute DSMZ (Braunschweig, Germany).
Wahlström A., Brumbaugh A., Sjöland W., Olsson L., Wu H., Henricsson M., Lundqvist A., Makki K., Hazen S.L., Bergström G., Marschall H.U., Fischbach M.A, & Bäckhed F. (2024). Production of deoxycholic acid by low-abundant microbial species is associated with impaired glucose metabolism. Nature Communications, 15, 4276.
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4

Multi-Strain Bacterial Reference Sample

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MSA-2002 was purchased from ATCC, Manassas, VA. The sample contains a mixture of 20 different bacterial strains distributed equally (5% ea.): Acinetobacter baumannii (ATCC 17978), Actinomyces odontolyticus (ATCC 17982), Bacillus cereus (ATCC 10987), Bacteroides vulgatus (ATCC 8482), Bifidobacterium adolescentis (ATCC 15703), Clostridium beijerinckii (ATCC 35702), Cutibacterium acnes (ATCC 11828), Deinococcus radiodurans (ATCC BAA-816), Enterococcus faecalis (ATCC 47077), Escherichia coli (ATCC 700926), Helicobacter pylori (ATCC 700392), Lactobacillus gasseri (ATCC 33323), Neisseria meningitidis (ATCC BAA-335), Porphyromonas gingivalis (ATCC 33277), Pseudomonas aeruginosa (ATCC 9027), Rhodobacter sphaeroides (ATCC 17029), Staphylococcus aureus (ATCC BAA-1556), Staphylococcus epidermidis (ATCC 12228), Streptococcus agalactiae (ATCC BAA-611), and Streptococcus mutans (ATCC 700610).
Deshpande S.V., Reed T.M., Sullivan R.F., Kerkhof L.J., Beigel K.M, & Wade M.M. (2019). Offline Next Generation Metagenomics Sequence Analysis Using MinION Detection Software (MINDS). Genes, 10(8), 578.
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5

Microbial Strain Preparation and Cultivation

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All microorganisms were obtained from the American Type Culture Collection (ATCC), the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) or the National Collection of Type Cultures (NCTC) and were stocked in calibrated cryotubes at -80°C after growing in broth. We used the following strains: Enterococcus faecalis ATCC 19433, Staphylococcus aureus DSMZ 799, Kocuria rhizophila ATCC 9341, Bacillus subtilis DSMZ 347, Bacillus cereus ATCC 14579, Bacteroides vulgatus ATCC 8482, Clostridium sporogenes ATCC 19404, Pseudomonas aeruginosa DSMZ 1128, Salmonella enterica subsp. enterica NCTC 6676, Escherichia coli DSMZ 1576, Proteus mirabilis ATCC 29906, Candida albicans DSMZ 1386 and Saccharomyces cerevisiae ATCC 7754.
For the micromanipulation of bacterial cells, microorganisms were plated on agar media after thawing the cryotube at room temperature. Aerobic bacteria were plated on TSA (Merck ref. 146004) and incubated 24h at 32.5°C; anaerobic bacteria were plated on Columbia blood agar (COS) (Merck ref. 146559) and incubated under anaerobic conditions (Genbox, Biomérieux ref. 96124) 24h to 48h at 32.5°C; and yeasts were plated on Sabouraud dextrose agar (SDA) (Merck ref. 146028) and incubated 48h to 72h at 22.5°C.
Hohnadel M., Maumy M, & Chollet R. (2018). Development of a micromanipulation method for single cell isolation of prokaryotes and its application in food safety. PLoS ONE, 13(5), e0198208.
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