Live-cell imaging experiments were performed on an inverted wide-field epi-fluorescence microscope Zeiss Axio Observer.Z1/7 (Carl Zeiss AG, Oberkochen, Germany) equipped with an LED light source Colibri 7 (423/44 nm, 469/38 nm, 555/30), Plan-Apochromat 20x/0.8 dry objective, Plan-Apochromat 40x/1.4 oil immersion objective, a monochrome C.C.D. camera Axiocam 503, and a custom-made gravity-based perfusion system. O-geNOps signals were imaged by exciting cells (555 nm) using a motorized dual filter wheel equipped with the filter combinations FT570 (BS) and emission filter 605/70. Control and data acquisition were executed using Zen Blue 3.1 Pro software (Carl Zeiss AG, Oberkochen, Germany). Administration and withdrawal of 3-(2-Hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-propanamine (NOC-7) were performed using a custom-made perfusion system connected to a metal perfusion chamber (NGFI, Graz, Austria).
Plan apochromat 20x 0 8 dry objective
Manufactured by Zeiss
Sourced in Germany
The Plan Apochromat 20x/0.8 dry objective is an optical lens component used in various microscopy applications. It provides a magnification of 20x and a numerical aperture of 0.8, which allows for high-resolution imaging and a wide field of view. The objective is designed to deliver a flat image field and superior color correction, making it suitable for a range of microscopy techniques.
Automatically generated - may contain errors
Lab products found in correlation
Axio observer z1, by Zeiss (3 mentions)
Ld c apochromat 40x 1.1 water immersion objective, by Zeiss (2 mentions)
Ld c apochromat 63x 1.4 oil immersion objective, by Zeiss (2 mentions)
Observer z1 inverted microscope, by Zeiss (2 mentions)
Zen 2012, by Zeiss (2 mentions)
Dmi8 inverted microscope, by Leica (2 mentions)
Zen blue 3.1 pro software, by Zeiss (1 mentions)
Plan apochromat 40x 1, by Zeiss (1 mentions)
Colibri 7, by Zeiss (1 mentions)
Axiocam 503, by Zeiss (1 mentions)
Zen 2.5 blue edition software, by Zeiss (1 mentions)
4 protocols using plan apochromat 20x 0 8 dry objective
1
Live-Cell Imaging of Nitric Oxide Signaling
2
Immunohistochemistry Image Acquisition and Analysis
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Image acquisition and analysis were performed at LiMiF https://limif.ulb.be/ , the Light Microscopy Facility, Université Libre de Bruxelles, Faculty of Medicine, Campus Erasme, Brussels. IHC slides were observed on a AxioObserver Z1 inverted microscope (Zeiss, Jena, Germany), using a Plan Apochromat 20x/0.8 dry objective (Zeiss). Transmitted light illumination was provided by a HAL100 halogen lamp and condenser in “bright-field position”. Images (1920 by 1216 pixels, pixel size (x-y): 0.293 micron by 0.293 micron) were acquired with an Axiocam 702 monochrome camera (Zeiss) as proprietary *.czi files. Files were processed with Zen 2.5 (Blue Edition) software (Zeiss). Images were displayed in the linear mode with manual contrast adjustment and exported as 16 bits uncompressed TIF files.
3
Confocal and STED Microscopy for Cellular Imaging
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Confocal imaging was performed on a LSM780NLO confocal system fitted on an Observer Z1 inverted microscope (Zeiss) equipped with a Chameleon Vision II 690-1064 nm multiphoton laser (Coherent Europe). Fluorochromes were separated by linear unmixing using ZEN 2012 software (Zeiss). IUE were imaged using a Plan Apochromat 20x/0,8 dry objective, in vivo cilia imaging was done using LD C Apochromat 40x/1.1 water immersion objective and in vitro cilia imaging was done using LD C Apochromat 63x/1.4 oil immersion objective (Zeiss). Wholemount cortices were flattened into fluorodish (Ibidi) and covered with glass coverslip before image acquisition using the 63x objective on 4-5 Z-stacks spaced at 0.3 μm to cover the whole tight-junction region.Images were then processed using the Fiji/ImageJ software (Schindelin et al., 2012 (link)).
Sted images were acquired using a DMi8 inverted microscope (Leica) equipped with STED with 592nm (CW), 660nm (CW) and 775nm (pulsed) depletion lasers. HC PL APO 100x/1.4 oil objective was used. Acquisition was done with LAS X and deconvolution using Huygens Professional Version 17.1. For STED imaging, ABBERIOR secondary antibodies were used.
Sted images were acquired using a DMi8 inverted microscope (Leica) equipped with STED with 592nm (CW), 660nm (CW) and 775nm (pulsed) depletion lasers. HC PL APO 100x/1.4 oil objective was used. Acquisition was done with LAS X and deconvolution using Huygens Professional Version 17.1. For STED imaging, ABBERIOR secondary antibodies were used.
4
Confocal and STED Imaging of Tight Junctions
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Confocal imaging was performed on a LSM780NLO confocal system fitted on an Observer Z1 inverted microscope (Zeiss) equipped with a Chameleon Vision II 690-1064 nm multiphoton laser (Coherent Europe). Fluorochromes were separated by linear unmixing using ZEN 2012 software (Zeiss) . In utero electroporation were imaged using a Plan Apochromat 20x/0,8 dry objective, in vivo cilia imaging was done using LD C Apochromat 40x/1.1 water immersion objective and in vitro cilia imaging was done using LD C Apochromat 63x/1.4 oil immersion objective (Zeiss). Wholemount cortices were flattened into fluorodish (Ibidi) and covered with glass coverslip before image acquisition using the 63x objective on 4-5 Z-stacks spaced at 0.3 µm to cover the whole tight-junction region.Images were then processed using the Fiji/ImageJ software.
Sted images were acquired using a DMi8 inverted microscope (Leica) equipped with STED with 592nm (CW), 660nm (CW) and 775nm (pulsed) depletion lasers. HC PL APO 100x/1.4 oil objective was used. Acquisition was done with LAS X and deconvolution using Huygens Professional Version 17.1. For Sted imaging, ABBERIOR secondary antibodies were used.
QUANTIFICATION AND STATISTICAL ANALYSIS.
Sted images were acquired using a DMi8 inverted microscope (Leica) equipped with STED with 592nm (CW), 660nm (CW) and 775nm (pulsed) depletion lasers. HC PL APO 100x/1.4 oil objective was used. Acquisition was done with LAS X and deconvolution using Huygens Professional Version 17.1. For Sted imaging, ABBERIOR secondary antibodies were used.
QUANTIFICATION AND STATISTICAL ANALYSIS.
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