Alginic acid sodium salt alg

Alginic acid sodium salt (ALG) with a molecular weight range: 120,000–190,000 g/mol, and a ratio of mannuronic to guluronic units (M/G): 1.53, was purchased from Sigma-Aldrich (St. Louis, MO, USA). ALG was additionally purified by preparing a solution of 7% w/v ALG in NaOH (0.005 M), which was repetitively purified by dialysis against ultrapure water (membrane MWCO: 12,000–14,000 Da, SERVA Electrophoresis, Heidelberg, Germany). The final ALG product was obtained in its solid state through lyophilization. The synthesis of the graft copolymer ALG-g-P(NIPAM₈₆-co-NtBAM₁₄) precursor and the amino-functionalized PNIPAM side chains, that were used for the synthesis of the heterograft ALG/HGC copolymer, have been previously described [36 (link)]. N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) was purchased from Alfa Aesar (Ward Hill, MA, USA) and 1-Hydroxybenzotriazole hydrate (HOBt) was obtained from Fluka (Charlotte, NC, USA). Sodium hydroxide (NaOH) was a product of Panreac (Chicago, IL, USA), and acetone and deuterated water (D₂O) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water was obtained by means of an ELGA Medica-R7/15 water purification device (ELGA Labwater, Woodridge, IL, USA).

KPF powder (90%) was prepared from Sigma-Aldrich (CAS.N 520183). We obtained 5-FU from Sigma, St. Louis, MO (USA). We prepared sodium tripolyphosphate (STPP) from Dae-Jung (Korea). We also bought chitosan (CS), pentylenetetrazol (PTZ), and alginic acid sodium salt (ALG), Sigma-Aldrich (St. Louis, Mo). Total antioxidant capacity (TAC) and malondialdehyde (MDA) kits were prepared from the Company of Teb Pajohan Razi (Iran). RNA extraction kit and (Sinacolon, Tehran, Iran) cDNA synthesis kit were bought from Sinacolon and Yekta Tajhiz Azma Company, respectively (Tehran, Iran).