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Small animal physiological monitoring system

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The small animal physiological monitoring system is a lab equipment designed to monitor the vital signs and physiological parameters of small animals during experiments or procedures. It provides real-time data on the animal's status, such as heart rate, respiration, and temperature, to assist researchers and veterinarians in maintaining the animal's well-being and ensuring the integrity of the research or medical intervention.

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Lab products found in correlation

Isoflurane, by Baxter (5 mentions) 39mm volume resonator rf coil, by Rapid Biomedical (2 mentions) Wild type c57bl 6 male mice, by Jackson ImmunoResearch (1 mentions) 9.4 t mri system, by Agilent Technologies (1 mentions) 9.4t mr system, by Agilent Technologies (1 mentions) Isoflurane gas, by Baxter (1 mentions) C57bl 6 wild, by Jackson ImmunoResearch (1 mentions) Mouse brain phased array surface coil, by Bruker (1 mentions) Wistar rat, by Charles River Laboratories (1 mentions) B ga 12s hp, by Bruker (1 mentions)

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Physiological monitoring system (6 citations)

16 protocols using small animal physiological monitoring system

1

In Vivo Neuroimaging of Mice

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All animal experiments and procedures were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of the Massachusetts General Hospital. Three C57/BL6 wild‐type male mice (27–31 gr) were purchased from Jackson Laboratory. They were anesthetized using 1%–2% isoflurane and placed on an MRI cradle with ear and bite bars to secure the head. Respiration rate was monitored with a small animal physiological monitoring system (SA Instruments, Stony Brook, NY), and the temperature was maintained by blowing warm air in the bore of the magnet.
Perlman O., Zhu B., Zaiss M., Rosen M.S, & Farrar C.T. (2022). An end‐to‐end AI‐based framework for automated discovery of rapid CEST/MT MRI acquisition protocols and molecular parameter quantification (AutoCEST). Magnetic Resonance in Medicine, 87(6), 2792-2810.
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2

High-Field MRI of Murine Subjects

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Imaging was performed at 5 months of age using a 9.4 T MRI system (Agilent Technologies, Santa Clara, USA) equipped with 1000mT/m gradient inserts and a 39mm volume resonator RF coil (RAPID Biomedical, Rimpar, Germany). A small animal physiological monitoring system (SA Instruments, Stony Brook, NY) was used to maintain depth of anesthesia and animal physiology. The ECG trace was recorded using 3-lead subcutaneous electrodes, respiration rate was measured by a pressure sensitive balloon and internal temperature by rectal thermometer. Animals were anesthetized under a mixture of 1–2% isoflurane in oxygen.
Jackson L.H., Vlachodimitropoulou E., Shangaris P., Roberts T.A., Ryan T.M., Campbell-Washburn A.E., David A.L., Porter J.B., Lythgoe M.F, & Stuckey D.J. (2017). Non-invasive MRI biomarkers for the early assessment of iron overload in a humanized mouse model of β-thalassemia. Scientific Reports, 7, 43439.
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3

In vivo Cardiac and Spleen Assessment

Cited 1 time
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In vivo imaging, spleen volume, cardiac function and relaxometry were performed as described in L. Jackson et al.23 (link), at 30 weeks of age using a 9.4T MR system (Agilent Technologies, Santa Clara, USA) equipped with 1000 mT/m gradient inserts and a 39mm volume resonator RF coil (RAPID Biomedical, Rimpar, Germany). A small animal physiological monitoring system (SA Instruments, Stony Brook, NY) was used to record the ECG trace, respiration rate, and internal temperature. Animals were anaesthetized with a mixture of isoflurane and oxygen with physiological measurements used to maintain the depth of anaesthesia23 (link).
Shangaris P., Loukogeorgakis S.P., Subramaniam S., Flouri C., Jackson L.H., Wang W., Blundell M.P., Liu S., Eaton S., Bakhamis N., Ramachandra D.L., Maghsoudlou P., Urbani L., Waddington S.N., Eddaoudi A., Archer J., Antoniou M.N., Stuckey D.J., Schmidt M., Thrasher A.J., Ryan T.M., De Coppi P, & David A.L. (2019). In Utero Gene Therapy (IUGT) Using GLOBE Lentiviral Vector Phenotypically Corrects the Heterozygous Humanised Mouse Model and Its Progress Can Be Monitored Using MRI Techniques. Scientific Reports, 9, 11592.
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4

Quantifying Tumor Burden in Mouse Lung MRI

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Mouse scans were performed using a 9.4T 20-cm bore Bruker Biospec scanners (Bruker Biospin MRI GmbH) equipped with an ID 114 mm maximum strength of 530 mT/m Bruker gradient. An ID 40 mm Bruker volume resonator was used for RF excitation and MRI acquisition. The mice were anesthetized with 2% isoflurane (Baxter Healthcare Corp) gas in oxygen during MRI scanning. A small animal physiological monitoring system (SA Instruments) was used to monitor animal respiration during MRI scanning. Scout images along three orthogonal orientations were first acquired for animal positioning. For mouse lung imaging, respiratory gated T1-weighted axial images using the FLASH gradient echo sequence were acquired with TR 170 ms, TE 1.6 ms, slice thickness of 0.8 mm, FOV 35 × 25 mm, in-plane resolution of 182 × 130 um, and 10 averages. The tumors were measured from their Digital Imaging and Communications in Medicine (DICOM) files using Image J software. Tumor burden was calculated by outlining the region of interest (ROI) of tumor structures, taking the output of the ROIs in mm2 and multiplying each slice’s ROI by its slice thickness.
Ciampricotti M., Karakousi T., Richards A.L., Quintanal-Villalonga À., Karatza A., Caeser R., Costa E.A., Allaj V., Manoj P., Spainhower K.B., Kombak F.E., Sanchez-Rivera F.J., Jaspers J.E., Zavitsanou A.M., Maddalo D., Ventura A., Rideout WM I.I.I., Akama-Garren E.H., Jacks T., Donoghue M.T., Sen T., Oliver T.G., Poirier J.T., Papagiannakopoulos T, & Rudin C.M. (2021). Rlf-Mycl gene fusion drives tumorigenesis and metastasis in a mouse model of small cell lung cancer. Cancer discovery, 11(12), 3214-3229.
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5

T2* MRI of Mouse Brain Imaging

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T2* MRI of the mice was carried out on the 300 MHz Bruker 4.7T Biospec scanners (Bruker Biospin MRI GmbH, Ettlingen, Germany) equipped with 640 mT/m ID 115 mm gradient (Resonance Research, Inc., Billerica, MA). RF excitation and acquisition was achieved by a custom-built quadrature birdcage resonator with an inner diameter (ID) of 32 mm (Stark Contrast MRI Coils Research Inc., Erlangen, Germany). The mice were anesthetized with 2% isoflurane (Baxter Healthcare Corp., Deerfield, IL) gas in oxygen. Animal respiration was monitored with a small animal physiological monitoring system (SA Instruments, Inc., Stony Brook, New York, USA). Scout images along three orthogonal orientations were first acquired for animal positioning. We used 3D Multiple Gradient Echo sequence (MGE) to acquire a series of T2*-weighted images with increasing echo time (TE) values 3.3 ms, 7.5ms, 11.4ms and 16.0 ms. Other acquisition parameters were repetition time (TR) 34 ms, field of view (FOV) 30 × 35 × 100 mm with a voxel size of 0.23 × 0.21 × 0.20 mm^3, 3 averages.
Aras O., Pearce G., Watkins A.J., Nurili F., Medine E.I., Guldu O.K., Tekin V., Wong J., Ma X., Ting R., Unak P, & Akin O. (2018). An in-vivo pilot study into the effects of FDG-mNP in cancer in mice. PLoS ONE, 13(8), e0202482.
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6

High-Resolution 7T MRI Imaging of Mouse Brain

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MR images were acquired on a dedicated small animal MRI scanner, consisting of a 7 Tesla superconducting magnet (Bruker Biospin Corp., Billerica, MA) and a gradient (Resonance Research Inc., Billerica, MA) with a clear bore size of 115 mm and maximum gradient amplitude of 640 mT/m. A custom-made 36-mm quadrature birdcage radiofrequency (RF) coil (Starks Contrast MRI coils Research Inc, Erlangen, Germany) was used for RF excitation and detection. Mice were immobilized by 2% isoflurane gas (Life Science, LLC, N Augusta, SC) in oxygen. Animal respiration was monitored with a small animal physiological monitoring system (SA Instruments, Inc., Stony Brook, New York). Scout images were acquired along three orthogonal orientations for animal positioning. To image the mouse brain, a brain coronal T2-weighted Rapid Acquisition with Relaxation Enhancement (RARE) fast spin echo sequence was used with the following parameters: 256×160 matrix, field of view (FOV) 3×2 cm, repetition time/echo time (TR/TE) of 1500/50 ms, 1 mm slice thickness, and 12 acquisitions in average. The total imaging time was six minutes.
Rotter L.K., Berisha N., Hsu H.T., Burns K.H., Andreou C, & Kircher M.F. (2022). Visualizing surface marker expression and intratumoral heterogeneity with SERRS-NPs imaging. Nanotheranostics, 6(3), 256-269.
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7

Noninvasive Optical In Vivo Colon Imaging

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Noninvasive, optical in vivo imaging was conducted using a Xenogen IVIS Spectrum. The IVIS Imaging System was prepared for use according to the IACUC Guidelines for Animal Use at MSKCC. Mouse colon MRI scans were performed on a 300 MHz Bruker 7T Biospec scanners (Bruker Biospin MRI GmbH, Ettlingen, Germany) equipped with 640 mT/m ID 115 mm gradients (Resonance Research, Inc., Billerica, MA). RF excitation and acquisition was achieved by a custom-built quadrature birdcage resonator with ID of 32 mm (Stark Contrast MRI Coils Research Inc., Erlangen, Germany). The mice were anesthetized with 1% isoflurane (Baxter Healthcare Corp., Deerfield, IL) gas in oxygen. Animal respiration was monitored with a small animal physiological monitoring system (SA Instruments, Inc., Stony Brook, New York). Scout images along three orthogonal orientations were first acquired for animal positioning. For colon imaging, axial T2-weighted images using fast spin-echo RARE sequence (Rapid Acquisition with Relaxation Enhancement) was acquired with TR 5.1, TE 47 ms, RARE factor of 8, slice thickness of 0.8 mm, FOV 30 mm, in-plane resolution of 117 × 156 um, and 8 averages.
O’Rourke K.P., Loizou E., Livshits G., Schatoff E.M., Baslan T., Manchado E., Simon J., Romesser P., Leach B., Han T., Pauli C., Beltran H., Rubin M.A., Dow L.E, & Lowe S.W. (2017). Transplantation of engineered organoids enables rapid generation of metastatic mouse models of colorectal cancer. Nature biotechnology, 35(6), 577-582.
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8

Noninvasive Optical In Vivo Colon Imaging

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Noninvasive, optical in vivo imaging was conducted using a Xenogen IVIS Spectrum. The IVIS Imaging System was prepared for use according to the IACUC Guidelines for Animal Use at MSKCC. Mouse colon MRI scans were performed on a 300 MHz Bruker 7T Biospec scanners (Bruker Biospin MRI GmbH, Ettlingen, Germany) equipped with 640 mT/m ID 115 mm gradients (Resonance Research, Inc., Billerica, MA). RF excitation and acquisition was achieved by a custom-built quadrature birdcage resonator with ID of 32 mm (Stark Contrast MRI Coils Research Inc., Erlangen, Germany). The mice were anesthetized with 1% isoflurane (Baxter Healthcare Corp., Deerfield, IL) gas in oxygen. Animal respiration was monitored with a small animal physiological monitoring system (SA Instruments, Inc., Stony Brook, New York). Scout images along three orthogonal orientations were first acquired for animal positioning. For colon imaging, axial T2-weighted images using fast spin-echo RARE sequence (Rapid Acquisition with Relaxation Enhancement) was acquired with TR 5.1, TE 47 ms, RARE factor of 8, slice thickness of 0.8 mm, FOV 30 mm, in-plane resolution of 117 × 156 um, and 8 averages.
O’Rourke K.P., Loizou E., Livshits G., Schatoff E.M., Baslan T., Manchado E., Simon J., Romesser P., Leach B., Han T., Pauli C., Beltran H., Rubin M.A., Dow L.E, & Lowe S.W. (2017). Transplantation of engineered organoids enables rapid generation of metastatic mouse models of colorectal cancer. Nature biotechnology, 35(6), 577-582.
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9

Multimodal Imaging of Probe Dynamics

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For all animals, images were acquired prior to, during, and post injection of probe. Animals were anesthetized with 1–2% isoflurane and air/oxygen mixture to maintain constant respiration rate at 60 breaths/minute monitored by a small animal physiological monitoring system (SA Instruments Inc., Stony Brook NY). Animals were placed in the scanner the following images were acquired: axial and coronal 2-dimensional (2D) Rapid Acquisition with Relaxation Enhancement (RARE) images, T1w 3D Fast Low Angle Shot (FLASH) images, and T1w 3D Ultra Short Time to Echo (UTE) images. During and immediately following 0.1 mmol kg–1 probe injection, a series of 10 T1w FLASH images were acquired to monitor probe injection and clearance. UTE images were then acquired again at 15, 30 and 45 min post injection. Sequence parameter details are outlined in the ESI.†
Akam E.A., Abston E., Rotile N.J., Slattery H.R., Zhou I.Y., Lanuti M, & Caravan P. (2019). Improving the reactivity of hydrazine-bearing MRI probes for in vivo imaging of lung fibrogenesis †Electronic supplementary information (ESI) available. See DOI: 10.1039/c9sc04821a. Chemical Science, 11(1), 224-231.
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10

Kidney Imaging using Murine MRI

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Mice kidney MRI were carried out on 400 MHz Bruker 9.4T Biospec scanners (Bruker Biospin MRI GmbH, Ettlingen, Germany) equipped with a 530 mT/m ID 114 mm gradient. RF excitation and acquisition were achieved by a Bruker quadrature birdcage resonator with ID of 40 mm. The mice were immobilized with 2% isoflurane (Baxter Healthcare Corp., Deerfield, IL) gas in oxygen. Animal respiration was monitored with a small animal physiological monitoring system (SA Instruments, Inc., Stony Brook, New York). Scout images along three orthogonal orientations were first acquired for animal positioning. For mouse kidney imaging, coronal T2-weighted images using respiratory gated fast spin-echo RARE sequence (Rapid Acquisition with Relaxation Enhancement) was acquired with TR 2s, TE 33 ms, RARE factor of 8, slice thickness of 0.4 mm, FOV 30 mm, in-plane resolution of 117 × 117 um, and 10 averages.
Dong Y., Gong Y., Kuo F., Makarov V., Reznik E., Nanjangud G.J., Aras O., Zhao H., Qu R., Fagin J.A., Sherman E.J., Xu B., Ghossein R., Chan T.A, & Ganly I. (2021). Targeting the mTOR Pathway in Hurthle Cell Carcinoma Results in Potent Anti-Tumor Activity. Molecular cancer therapeutics, 21(2), 382-394.
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