Trypan blue solution t8154

CEP-1347 was purchased from TOCRIS Bioscience (Bristol, UK) and dissolved in DMSO to prepare a 0.5 mM stock solution. RG7112 was purchased from Selleck Chemicals (Houston, TX, USA) and dissolved in DMSO to prepare a 5 mM stock solution. Trypan blue solution (T8154) was obtained from Merck KGaA (Darmstadt, Germany). An antibody against murine double minute 4 (MDM4, A700-000-T) was purchased from BETHYL (FORTIS LIFE SCIENCES, Waltham, MA, USA); an antibody against MDM2 (AF1244) was from R&D Systems (Minneapolis, MN, USA); the antibodies against cyclin-dependent kinase inhibitor 1A (CDKN1A, p21^Waf1/Cip1) (#2947) and GAPDH (#5174) were from Cell Signaling Technology, Inc. (Beverly, MA, USA); and an antibody against p53 (sc-126) was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Domatinostat (4SC-202; S7555) was purchased from Selleck Chemicals (Houston, TX, USA). Domatinostat was dissolved in DMSO to prepare a 10 mM stock solution. The structure of domatinostat was obtained from DrugBank [38 (link)]. Propidium iodide (P3566) and Hoechst33342 (H3570) solutions were purchased from Thermo Fisher Scientific, (Waltham, MA, USA). Trypan blue solution (T8154) was purchased form Merck (Darmstadt, Germany). Antibodies against Bmi1 (05-637) and Nestin (MAB5326) were purchased from Merck. An antibody against SOX2 (MAB2018) was purchased from R&D Systems Inc. (Minneapolis, MN, USA). Anti-CD133 (W6B3C1) was purchased from Miltenyi Biotech (Bergisch Gladbach, Germany). Antibodies against, OCT-4a (#2890), GAPDH (#5174), cleaved PARP (#9541), and cleaved caspase 3 (#9661) were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA).