Waters 2489 uv visible detector

Manufactured by Waters Corporation

Sourced in United States

The Waters 2489 UV/Visible detector is a laboratory instrument designed to measure the absorbance of light by a sample. It is capable of detecting and quantifying the amount of ultraviolet (UV) or visible light absorbed by a substance, which can be used to identify and analyze various chemical compounds.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

2489 UV/Visible Detector (40 citations) Waters 2489 (38 citations) UV detector (22 citations) Waters 2489 UV detector (18 citations) 2489 UV detector (18 citations) Waters 2489 detector (3 citations) Waters 2489 UV (2 citations)

19 protocols using waters 2489 uv visible detector

HPLC Analysis of 5-MedC and dC

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC analysis was performed on a liquid chromatograph coupled with a Waters 2489 UV/visible detector (Waters Corporation, Milford, MA, USA). Separation was achieved on a Symmetry^® C18 (5 µm; 4.6×250) column (Waters Corporation). The mobile phase consisted of solvent A (50 mM ammonium formate; pH 5.4) and solvent B (HPLC-grade acetonitrile). The following gradient program used was: Solvent B 2% at 0 min, solvent B 3% at 18 min, solvent B 27% at 25 min, solvent B 35% at 40 min and solvent B 2% at 45 min. The total duration of analysis was 50 min. The flow rate was set at 0.2 ml/min and the column oven temperature was maintained at 25°C. UV detection was performed at 277 nm.
The concentrations of MedC and dC were calculated from linear regression curves, and the percentage of methylation was then calculated using the following equation: 5-MedC (%)=[5-MedC/(dC+MedC)]x100.

Teng S., Wang Y., Li P., Liu J., Wei A., Wang H., Meng X., Pan D, & Zhang X. (2017). Effects of R type and S type ginsenoside Rg3 on DNA methylation in human hepatocarcinoma cells. Molecular Medicine Reports, 15(4), 2029-2038.

+ Open protocol

+ Expand

HPLC Identification of Antioxidant Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

To confirm the identity of the antioxidant compounds corresponding to the structure identified by the NMR measurement, HPLC was performed using Lee et al.’s method [36 (link)]. Samples and standards were added to the methanol, followed by filtration through a 0.45-μm membrane filter (Millipore Co., Burlington, MA, USA) and analysis by HPLC (Waters e2695, Waters Co., Milford, MA, USA). The column comprised Capcell pak C₁₈ UG120, S5 (4.6 × 250 mm) (Shiseido co., LTD, Tokyo, Japan). The eluents were 0.1% formic acid in water (A) and 0.1% formic acid in 90% acetonitrile with water (B) and the flow rate used was 0.1 mL/min. The gradient was as follows: 0–4 min, 10% B; 4–20 min, 60% B; 20–23 min, 10% B; 23–30 min, 10% B. The injection volume was 10 µL and the detection wavelength was 310 nm (Waters 2489 UV/visible detector, Waters Co., Milford, MA, USA).

Kim S.H, & Huh C.K. (2022). Isolation and Identification of Fisetin: An Antioxidative Compound Obtained from Rhus verniciflua Seeds. Molecules, 27(14), 4510.

+ Open protocol

+ Expand

Synthesis and Characterization of STR-His16 Peptide

Check if the same lab product or an alternative is used in the 5 most similar protocols

The STR-His16 peptide was purchased from Biologica Co. (Shanghai, China). His16 peptide was synthesized by solid-phase peptide synthesis, and STR-His16 peptide was prepared by treatment of the His16 peptide on resins with stearic acid (C₁₇H₃₅COOH) and diisopropylcarbodiimide in the presence of N-hydroxybenzotriazole. STR-His16 peptide with a stearyl moiety at the N-terminus and an amide group at the C-terminus was purified by high-performance liquid chromatography (HPLC) using a Waters 2489 UV/visible detector and 1524 binary pump (Waters, Milford, MA, USA) and a reversed phase COSMOSIL 5C₁₈-MS-II column (10 mm × 250 mm; Nacalai Tesque, Kyoto, Japan) as previously described [4 (link)]. The column was run for 40 min at 3 mL/min with a linear gradient from 0% to 80% (v/v) acetonitrile in water containing 0.1% (v/v) trifluoroacetic acid. The purity of STR-His16 peptide was calculated from peak areas of HPLC chart, and final purity of the peptide was >95% (Figure S1A). The molecular masses were confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (AutoFlex II; Bruker Daltonics, Billerica, MA, USA) (Figure S1B).

Hayashi T., Okamoto R., Kawano T, & Iwasaki T. (2019). Development of Organelle Replacement Therapy Using a Stearyl-Polyhistidine Peptide against Lysosomal Storage Disease Cells. Molecules, 24(16), 2995.

+ Open protocol

+ Expand

Peptide Separation by HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

For separation of peptides contained in protein hydrolysates, HPLC was utilized. Waters 1525 Binary HPLC pump (Wasters, Milford, MA, USA), Waters 2489 UV/Visible detector (Waters), and Sunfire C18 column (4.6 × 250 mm) were used. The mobile phase was an isocratic combination of acetonitrile:H₂O (50:50) with a flow rate of 1 mL/min. Following the real-time UV detection result, the elutes were respectively collected.

Lee S, & Youn B. (2020). Hypolipidemic Roles of Casein-Derived Peptides by Regulation of Trans-Intestinal Cholesterol Excretion and Bile Acid Synthesis. Nutrients, 12(10), 3058.

+ Open protocol

+ Expand

Quantifying Residual p-CA and Radicals

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fluid in the Petri plate was collected after 1, 2, 3, 5, and 7 days of germination to evaluate residual p-CA concentrations. A certain amount of ddH₂O was added to the Petri dish to maintain the same weight as at the beginning of the experiment. The extracted liquid was then filtered through 0.22 μm Millipore membrane filters. The p-CA concentration in the filtrate was measured using a Waters Alliance e2695 HPLC system equipped with a Waters 2489 UV/visible detector (Waters Co., Milford, MA, USA) and a C18 column (5.0 m, 4.6 mm 250 mm). The detection wavelength was adjusted at 288 nm. The mobile phase was a 70:30 volume mixture of methanol and water containing 0.1% formic acid, with a flow rate of 0.6 mL/min. The free radicals such as sulfate radical (SO₄^•−), hydroxyl radical (HO^•), superoxide radical (O₂^•−), and singlet oxygen (¹O₂) were detected using electron paramagnetic resonance (EPR) on A300-10-12 spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany) with 0.1 mol/L DMPO and TEMP as spintrapping agents.

Tu Y., Shen J., Peng Z., Xu Y., Li Z., Liang J., Wei Q., Zhao H, & Huang J. (2023). Biochar-Dual Oxidant Composite Particles Alleviate the Oxidative Stress of Phenolic Acid on Tomato Seed Germination. Antioxidants, 12(4), 910.

+ Open protocol

+ Expand

HPLC Analysis of Indigo and Indirubin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Standard solutions containing 1 µg/ml concentrations of indigo and indirubin in DMSO were prepared and diluted with 1:1 DMSO:acetonitrile mixtures to concentrations of 1, 5 and 10 ng/ml. Standard stock solutions and working solutions were stored at 4°C. For preparation of the sample, samples of PR extract were weighed, and dissolved in 1:1 DMSO and acetonitrile mixtures. The samples were then sonicated for 1 h at room temperature. Prior to high performance liquid chromatographic system (HPLC) analysis, the sample preparations were filtered through a 0.45-µm membrane filter. A Waters Alliance HPLC system (Waters Corporation, Milford, MA, USA), equipped with a Waters 2489 UV/Visible detector was used for analysis. An Empower Data System and YMC-Pack Pro C-18 column (1.7 µm, 2.1×100 mm; both Waters Corporation) were used for recording the output signal of the detector and for separation, respectively. The mobile phase consisted of 0.1% aqueous formic acid and 0.1% formic acid in acetonitrile. The flow rate in the gradient elution system was 1.0 ml/min, the injection volume was 10 µl, the UV detection wavelength was 540 nm and the column was maintained at room temperature.

LEE W.H., CHOI S.H., KANG S.J., SONG C.H., PARK S.J., LEE Y.J, & KU S.K. (2016). Genotoxicity testing of Persicariae Rhizoma (Persicaria tinctoria H. Gross) aqueous extracts. Experimental and Therapeutic Medicine, 12(1), 123-134.

+ Open protocol

+ Expand

HPLC Analysis of Drug Concentrations

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HPLC apparatus consisted of a Waters 2695 Separation Module (Waters, Tokyo, Japan) and a Waters 2489 UV/Visible Detector (Waters), and the measured data were recorded and analyzed using Empower analysis software (Waters). We used an Inertsil C18 ODS-3 column (mean particle size: 5 μm, 4.6 × 250 mm, GL Sciences Inc., Tokyo, Japan). The drug concentrations were calculated based on the absolute calibration curve method, and the response was found to be linear over the calibration range (from 4 µM to 100 µM) with a correlation coefficient of 0.999 (Table 3).

Ikarashi N., Nagoya C., Kon R., Kitaoka S., Kajiwara S., Saito M., Kawabata A., Ochiai W, & Sugiyama K. (2019). Changes in the Expression of Aquaporin-3 in the Gastrointestinal Tract Affect Drug Absorption. International Journal of Molecular Sciences, 20(7), 1559.

+ Open protocol

+ Expand

HPLC Quantification of Oleanolic Acid

Check if the same lab product or an alternative is used in the 5 most similar protocols

A Waters HPLC system including Pump e2695 Separations Module and Waters 2489 UV/ Visible Detector (Waters Corp., Milford, MA, USA) was used to determine the concentrations of OA. A COSMOSIL 5 C18-AR-II column (150 mm × 4.6 mm, 5 µm) was used for the separation. Mobile phase composed of methanol and purified water containing 0.1% phosphoric acid (90:10, v/v) was used at a flow rate of 1.0 ml/min. The column temperature was maintained at room temperature. The wavelength of the UV detector was set at 210 nm. The calibration curve of OA concentrations (C, 4.09–204.5 µg/ml) versus integrated area (A, mAUs) was plotted and the linear fit A = 9238.8C+4458.3 was obtained with a correlation coefficient of 0.9998.

Zhang W., Liang C., Liu H., Li Z., Chen R., Zhou M., Li D., Ye Q., Luo C, & Sun J. (2017). Polymeric nanoparticles developed by vitamin E-modified aliphatic polycarbonate polymer to promote oral absorption of oleanolic acid. Asian Journal of Pharmaceutical Sciences, 12(6), 586-593.

+ Open protocol

+ Expand

HPLC Piperine Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC analyses were performed on all samples to ascertain the piperine
content using a Waters HPLC equipped with Empower software to analyze the data.
HPLC consisted of a Waters e2695 separation Module and Waters 2489 UV/Visible
detector, (Waters Technologies Corporation, 34 Maple St., Milford, MA 0157). The
column used was Phenomenex Luna C18 (5 μ, 250 × 4.6 mm). The
mobile phase consisted of a mixture of 0.1% ortho phosphoric acid and
acetonitrile (45:55 v/v) with a flow rate of 1.2 mL/min and 20 μL
injection volume. The column temperature was 35°C (±2). The UV
detector wavelength for piperine detection was set at λ_max 262
nm [23 ].

Ashour E.A., Majumdar S., Alsheteli A., Alshehri S., Alsulays B., Feng X., Gryczke A., Kolter K., Langley N, & Repka M.A. (2016). Hot melt extrusion as an approach to improve solubility, permeability and oral absorption of a psychoactive natural product, piperine. The Journal of Pharmacy and Pharmacology, 68(8), 989-998.

+ Open protocol

+ Expand

Analytical Characterization of Synthetic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Starting materials and other reagents were purchased from commercial suppliers and were used without further purification unless otherwise noted. All reactions were monitored by thin layer chromatography (TLC) with 0.25 mm E. Merck pre-coated silica gel plates (60 F254) and Waters LCMS system (Waters 2489 UV/Visible Detector, Waters 3100 Mass, Waters 515 HPLC pump, Waters 2545 Binary Gradient Module, Waters Reagent Manager, Waters 2767 Sample Manager) using SunFireTM C18 column (4.6 × 50 mm, 5 μm particle size): solvent gradient = 100% A at 0 min, 1% A at 5 min; solvent A = 0.035% TFA in Water; solvent B = 0.035% TFA in CH3CN; flow rate : 2.5 mL/min. Purification of reaction products was carried out by flash chromatography using CombiFlash®Rf with Teledyne Isco RediSep®Rf High Performance Gold or Silicycle SiliaSepTM High Performance columns (4 g, 12 g, 24 g, 40 g, 80 g, or 120 g). The purity of all compounds was over 95% and was analyzed with Waters LCMS system. ¹H NMR and ¹³C NMR spectra were obtained using a Varian Inova-400 (400 MHz for 1H, and 75 MHz for 13C) spectrometer. Chemical shifts are reported relative to chloroform (δ = 7.24) for ¹H NMR or dimethyl sulfoxide (δ = 2.50) for ¹H NMR and dimethyl sulfoxide (δ = 39.51) for ¹³C NMR. Data are reported as (br = broad, s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet).

Hatcher J.M., Bahcall M., Choi H.G., Gao Y., Sim T., George R., Jänne P.A, & Gray N.S. (2015). Discovery of inhibitors that overcome the G1202R ALK Resistance Mutation. Journal of medicinal chemistry, 58(23), 9296-9308.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!