Eclipse plus c18 column

All chemical reagents were commercially available. Silica gel column chromatography was performed on silica gel 60, 230–400 mesh, Merck. Nuclear magnetic resonance (¹H NMR and ¹³C NMR) spectra were recorded on JEOL JNM-LA 300 [300 MHz (¹H), 75 MHz (¹³C)] and Bruker Avance 400 MHz FT-NMR [400 MHz (¹H), 100 MHz (¹³C)] spectrometers. Chemical shifts are reported in ppm units with Me₄Si as a reference standard. Mass spectra were recorded on a VG Trio-2 GC-MS and 6460 Triple Quad LC/MS. All final compounds were purified to >95% purity, as determined by high-performance liquid chromatography (HPLC). HPLC was performed on an Agilent 1120 Compact LC (G4288A) instrument using an Agilent Eclipse Plus C18 column (4.6 × 250 mm, 5 μm) and a Daicel Chiralcel OD-H column (4.6 × 250 mm, 5 μm).

All chemical reagents were commercially available. Melting points were determined on a Büchi Melting Point B-540 apparatus and are uncorrected. Silica gel column chromatography was performed on silica gel 60, 230–400 mesh, Merck. Nuclear magnetic resonance (¹H NMR and ¹³C NMR) spectra were recorded on JEOL JNM-LA 300 [300 MHz (¹H), 75 MHz (¹³C)] and Bruker Avance 400 MHz FT-NMR [400 MHz (¹H), 100 MHz (¹³C)] spectrometers. Chemical shifts are reported in ppm units with Me4Si as a reference standard. Mass spectra were recorded on a VG Trio-2 GC–MS and 6460 Triple Quad LC/MS. All final compounds were purified to >95% purity, as determined by high-performance liquid chromatography (HPLC). HPLC was performed on an Agilent 1120 Compact LC (G4288A) instrument using an Agilent Eclipse Plus C18 column (4.6 × 250 mm, 5 μm) and a Daicel Chiralcel OD-H column (4.6 × 250 mm, 5 μm).

All chemical reagents were commercially available. Melting points were determined on a Büchi Melting Point B-540 apparatus and are uncorrected. Silica gel column chromatography was performed on silica gel 60, 230–400 mesh, Merck. Nuclear magnetic resonance (¹H NMR and ¹³C NMR) spectra were recorded on JEOL JNM-LA 300 [300 MHz (¹H), 75 MHz (¹³C)] and Bruker Avance 400 MHz FT-NMR [400 MHz (¹H), 100 MHz (¹³C)] spectrometers. Chemical shifts are reported in ppm units with Me₄Si as a reference standard. Infrared (IR) spectra were recorded on a JASCO FT/IR-4200 spectrometer. Mass spectra were recorded on a VG Trio-2 GC-MS and 6460 Triple Quad LC/MS. All final compounds were purified to >95% purity, as determined by high-performance liquid chromatography (HPLC). HPLC was performed on an Agilent 1120 Compact LC (G4288A) instrument using an Agilent Eclipse Plus C18 column (4.6 × 250 mm, 5 μm) and a Daicel Chiralcel OD-H column (4.6 × 250 mm, 5 μm).