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Secondary anti rabbit and or anti mouse antibodies

Manufactured by Merck Group

Secondary anti-rabbit and/or anti-mouse antibodies are laboratory reagents used to detect the presence of primary antibodies raised against rabbit or mouse antigens in immunoassays and other applications. These antibodies are designed to bind to the Fc region of the primary antibodies, allowing for the visualization or detection of the target antigen.

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2 protocols using secondary anti rabbit and or anti mouse antibodies

1

Histone Modifications in Brain Regions

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Frozen tissue punches of the relevant regions were homogenized and boiled in 1% SDS. For clozapine and quinpirole treatments tissues were obtained 24h after the last injection. Extracts (50 μg) were separated on SDS-PAGE and transferred onto membranes. Antibody used were: anti-H3K9me2 (1:5000), anti-H3K9me3 (1:1000) from Active Motif; anti-H3K4me3 (1:1000), anti-H3 (1:1000) from Abcam; anti-GAPDH (1:20,000) Millipore; anti-Akt1 (1:1000) Cell Signaling; anti-NR4A2 (1:1000) Antibody Verify. Secondary anti-rabbit and/or anti-mouse antibodies (1:5000) were from Millipore. Western blots were revealed using ECL Plus (GE Healthcare or Westdura, Pierce). Quantifications were performed using the NIH ImageJ (version 1.42q) software.
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2

Histone Modifications in Brain Regions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen tissue punches of the relevant regions were homogenized and boiled in 1% SDS. For clozapine and quinpirole treatments tissues were obtained 24h after the last injection. Extracts (50 μg) were separated on SDS-PAGE and transferred onto membranes. Antibody used were: anti-H3K9me2 (1:5000), anti-H3K9me3 (1:1000) from Active Motif; anti-H3K4me3 (1:1000), anti-H3 (1:1000) from Abcam; anti-GAPDH (1:20,000) Millipore; anti-Akt1 (1:1000) Cell Signaling; anti-NR4A2 (1:1000) Antibody Verify. Secondary anti-rabbit and/or anti-mouse antibodies (1:5000) were from Millipore. Western blots were revealed using ECL Plus (GE Healthcare or Westdura, Pierce). Quantifications were performed using the NIH ImageJ (version 1.42q) software.
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